Abacus junior 5
User’s Manual
29
In the followings we summarize the
warning flags
and give an explanation of their
possible cause and a few hints to overcome the problem:
Uppercase letters refer to WBC or HGB problems:
Flag
Meaning
Recommended user action
E
No WBC 3-part differential Possible lyse problem. May occur in pathological
lymphocytosis.
H
HGB blank is high, or no
HGB blank
Repeat the blank measurement. If HGB blank is not stable
there are probably bubbles in the WBC chamber: Run a
cleaning and try blank again. Close the side door if open
during measurement.
B
WBC blank is high, or no
WBC blank
Repeat the blank measurement, or run prime lyse and try
blank again.
Possible lyse contamination, or noise problem.
C, Q
WBC clogging
Aperture clogging. Perform cleaning and repeat the
measurement. If it is a general problem, please contact your
Service Personnel.
Low temperature reagents can cause it as well (diluent), in
this case you will have to wait until the reagents warm up to
room temperature.
S
Slice error
The sample just run might be clogged, not well mixed, or has
too many cells. Try re-running the sample, or use pre-
dilution.
D
Data error
The measurement-processing unit encountered problems it
could not correct. If problem persists, contact service for
software or measurement unit revision.
M
More cells
There were too many cells in the WBC solution. Try running
the sample in pre-diluted mode.
R
Too many RBC’s in WBC
sample.
Too many RBC were calculated in the sample. The reason
can be an increased number of RBC’s or decreased
sensitivity for lyse reagent. A slight increase in the lyse
amount can help solving this problem.
L
WBC-RBC differentiation
warning
Too many RBC’s were left in the WBC region. Try increasing
lyse volume,
W
3 part differentiation fault
The sample did not react to the lyse reagent as expected,
lyse volume should be changed to give better separation for
cell populations
Table 3. Summary of warning flags related to WBC/HGB
