Dakewe(Shenzhen)Medical Equipment Co,. Ltd
37
V4.1
6.4 Sectioning
Turn the hand wheel (clockwise or counterclockwise) for one circle, and then slice once, the
slice thickness is the setting slice thickness value.
Precautions:
Install the blade after fixing the specimen first.
Use the lock function of the hand wheel in time.
Correctly use the protective function of the anti-roll glass plate and the knife guard;
Do not leave the blade on the blade holder after the operation.
6.5 Sectioning Reference
1. When using a cryostat, mastering the appropriate sectioning speed and properly adjusting
the anti-roll plate is the most important factor in cutting out the ideal tissue piece. The
suitable sectioning speed is mastered by practical experience, and it is realized by skillfully
operating the hand wheel. Adjust more parts of the anti-rolling plate, some of which affect
each other, and need to be carefully debugged, they cannot be placed on the blade.
2. When living tissue is frozen, the water condenses into ice, the tissue becomes hard, and its
hardness changes with temperature. The lower the temperature, the harder the tissue,
whichever tissue, at whatever temperature, whether they can obtain good quality slice, need
to be explored through practice, most of the sections without fat and without formalin fixed
tissue temperature, preferably between -13 ° C ~ -23 ° C.
3. In order to obtain high-quality tissue films, you must pay attention to the following aspects:
Suitable freezer working temperature selection.
Correct slicing operation.
Elaborate anti-roll plate adjustment.
Sharp slicing blade, suitable cutting angle.
4. The frozen section is opposite to the general paraffin section. The frozen section does not
cut the long side of the tissue. It is best to cut the short side of the tissue, that is, the contact
line between the tissue and the slicing blade is shorter.
