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Adjustable Oligo Column

Operating Instructions

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Содержание 18110760

Страница 1: ...Adjustable Oligo Column Operating Instructions cytiva com...

Страница 2: ...tion 21 5 2 Packthecolumn 22 5 3 Unpackandemptythecolumn 24 6 Maintenance 25 6 1 Disassembleandassembleforcleaningandmaintenance 26 6 2 Cleaning 27 6 3 Partreplacement 28 7 Troubleshooting 30 8 Refere...

Страница 3: ...Pageintentionallyleftblank...

Страница 4: ...formation intendeduseof AdjustableOligo Column andlistsofabbreviationsusedinthisdocumentation Inthischapter Section Seepage 1 1 Importantuserinformation 6 1 2 Aboutthismanual 7 1 3 Abbreviations 8 1 I...

Страница 5: ...leusingPrimerSupport Thecolumnisdesignedfor maximumscalabilityandcouplingefficiencywithaflexiblecolumnvolume AdjustableOligoColumn isintendedforusewith KTA OligoPilot plus100 AdjustableOligoColumn mus...

Страница 6: ...desinformationneededtoinstall operateand maintaintheproductinasafeway Scopeofthismanual Thismanualdescribeshowtouse AdjustableOligoColumn Theillustrationbelow shows AdjustableOligoColumn 1 Introductio...

Страница 7: ...iations Abbreviation Meaning CV Columnvolume EPDM Ethylenepropylenediene monomer ETFE Ethylene tetrafluoroethylene ID Internaldiameter OD Outerdiameter PEEK Polyetheretherketone PTFE Polytetrafluoroet...

Страница 8: ...ent Inthischapter Section Seepage 2 1 Safetyprecautions 10 2 2 Symbolsandabbreviations 14 Important WARNING Beforeinstalling operatingormaintainingtheproduct all usersmustreadandunderstandtheentirecon...

Страница 9: ...ion Maintenance Definitions Thisuserdocumentationcontainssafetynotices WARNING CAUTION andNOTICE concerningthesafeuseoftheproduct Seedefinitionsbelow WARNING WARNINGindicatesahazardoussituationwhich i...

Страница 10: ...as sufferedanydamage forexampleifdamageiscausedbydropping theequipment Personalprotection WARNING Hazardoussubstancesandbiologicalagents Whenusing hazardouschemicalandbiologicalagents takeallsuitable...

Страница 11: ...thesuppliedspannerorwrench DonotovertightenPTFEtubingasthiswilldamagetheendofthe tubing WARNING Neverusepressurelimitsabovethecolumnspecification WARNING Neverexceedtheoperatinglimitsstatedinthisdocum...

Страница 12: ...ggedastakenoutofoperation allprocesswettedareasarecleananddecontaminated WARNING Decontaminatebeforemaintenance Toavoidpersonnelbeing exposedtopotentiallyhazardoussubstances makesurethatthe columnispr...

Страница 13: ...ion Descriptionofsymbolsonthecolumn label Thefollowingsymbolsmaybepresentonthecolumnlabel Label Meaning FollowinstructionsforuseintheOperating Instructions manual Warning Readtheuserdocumentationbefor...

Страница 14: ...ods Note If the support rods between the two end pieces have been removed and refitted they must be tightened with a torque key to 1 45 to 1 55 Nm for safe operation Thebottomend piececontainsthebotto...

Страница 15: ...8 6 9 10 11 2 Part Function 1 Securingringadapter 2 Connectionpointtothecolumn 3 PrefilterunitincludingO ring 4 TubingD flange 5 O ring 64 5 3mmdiameter 3 Productdescription 16 AdjustableOligoColumnO...

Страница 16: ...ion 6 O ring 55 1 6mmdiameter 7 Glasscolumntube 8 Distributionunit 9 Securingring 10 Connectionpointfromthecolumn 11 Tierodandhex nut 3 Productdescription AdjustableOligoColumnOperatingInstructions289...

Страница 17: ...d with the adapter fitted in the column Unpacking Ondeliveryofthecolumn performthefollowingsteps Step Action 1 Opentheboxandcarefullyremovethecolumn 2 Inspectthecolumnforanydamage 3 Reportanydamagetoy...

Страница 18: ...iallydangerousifhazardouschemicalsareinuse Connectthetubingbyfirstinsertingthetubingfully then tighteningtheconnectorfingertight PEEKtubingshouldbe tightenedafurther1 4turnusingthesuppliedspannerorwre...

Страница 19: ...NOTICE Toavoidprecipitationkeepallreagentsatorabove18 C 5 Operation 20 AdjustableOligoColumnOperatingInstructions28967444AC...

Страница 20: ...Downflow Canhelpsomesupportstospreadthe reagentsmoreevenly Reagentsenterthe columnthroughthebottomfilterandexit throughthetopadapterfilter Usingflowdirectionfrombottomtotop alsosimplifieschangingadir...

Страница 21: ...hesystemside 2ofeach 18117264 18112117 FerrulesforOD1 8 tubing 4 18112118 Tubing1 ID1 6mmOD1 8 2 18112163 1 Cutthetubingtoasuitablelength Calculatecolumnvolume Fromthetablebelow calculatethecolumnvolu...

Страница 22: ...on 6 Adjustthecolumnadaptertothelevelthatcorrespondstothecalculated volumefromthelistinCalculate column volume on page 22 7 ConnectthesystemtotheinletofthecolumnusingaPTFEtubewithanM6 connector 181172...

Страница 23: ...mntoallow theacetonitriletocomeoutatthebottomofthecolumn 4 ContinuetheairflowuntilthePrimerSupporthasreducedinvolumeandis freefromthecolumnwall Note This will take approximately 30 minutes depending o...

Страница 24: ...y The AdjustableOligoColumn isdesignedwithaminimumofcomponentstofacilitate cleaningandoperation Inthischapter Section Seepage 6 1 Disassembleandassembleforcleaningandmaintenance 26 6 2 Cleaning 27 6 3...

Страница 25: ...lockwiseby handandpullitoutofthecolumnbody Assembly Toassemble AdjustableOligoColumn followthesesteps Step Action 1 Findtheguidescrewontheinsideofthecolumnbodyandthegrooveonthe outsideoftheadapter 2 I...

Страница 26: ...nbeforeperformingany maintenanceactivities service orreturningtheproduct Cleanthefrits Pumpmethanolslowlyfor30minutesthenleavetoletdry Note Do not sonicate the frits Testthecleaneddistributionplates I...

Страница 27: ...oppieceandtheblacksecuringO ring Lift theadapterpre filterhousingfree 4 Usetheplasticwrenchtodisconnectthelengthoftubingfromthepre filter housingthatconnectsthepre filterhousingtothedistributionunitat...

Страница 28: ...O ringreplacement TwodifferenttypesofO ringsareusedinthe AdjustableOligoColumn Useaspatula topryoutwornO rings 6 Maintenance 6 3 Partreplacement AdjustableOligoColumnOperatingInstructions28967444AC 29...

Страница 29: ...alblockage Cleanallvalvesand tubingandremoveany internalblockage Columnfiltersare blocked Airmaybe trappedinthefilter Alwayswetandflushwith 95 ethanolor acetonitrilebeforeuse Tubingisbent Straightenth...

Страница 30: ...ation regulatory information orderinginformation andHealthandSafetyDeclarationformsforservice Inthischapter Section Seepage 8 1 Specification 32 8 2 Chemicalresistance 33 8 3 Recyclinginformation 35 8...

Страница 31: ...m 1 Thedesignpressureisthesumofpackingandoperatingpressures Thedesignpressuremust notbeexceeded Note Using Primer Support 5G according to recommended packing procedures adds no packing pressure Dimens...

Страница 32: ...swettedpartsaremanufacturedfrom fluoroplastic borosilicateglass polypropylene polyethylene ETFE titanium Chemicals Theinformationinthetablebelowhasbeencompiledfrompublishedmaterialfrom severalsources...

Страница 33: ...tration Sodiumhydroxide 2M Trifluoroacetic acid 0 1 NOTICE DonotuseTHF tetrahydrofurane in AdjustableOligoColumn 8 Referenceinformation 8 2 Chemicalresistance 34 AdjustableOligoColumnOperatingInstruct...

Страница 34: ...theproduct Whentakingtheproductoutofservice thedifferentmaterialsmustbeseparatedand recycledaccordingtonationalandlocalenvironmentalregulations Recyclingofhazardoussubstances Theproductcontainshazardo...

Страница 35: ...troubleshootingreports visit cytiva com contact Manufacturinginformation Thetablebelowsummarizestherequiredmanufacturinginformation Requirement Information Nameandaddressofmanufacturer CytivaSwedenAB...

Страница 36: ...gadapter 1 18111572 Glasstube 1 18111533 Distributionunit 1 29042220 Securingring60 1 18111573 TierodincludingHex nut 1 18111534 ETFEtubingID1 6mm OD1 8 3m 1 18112116 Tubingtoadjustablecolumn 1 181115...

Страница 37: ...e cases this may require customer to move equipment from normal operating location prior to Cytiva arrival Consumables such as columns or gels have been removed or isolated from the instrument and fro...

Страница 38: ...t be decontaminatedprior to service return Provide a telephone number where Cytiva can contact you for additional information concerning the system equipment Telephone No Liquid and or gas in equipmen...

Страница 39: ...ion 36 N Notesandtips 6 O O ringreplacement 29 Onsiteservice 38 P Packcolumn 22 23 calculations 22 procedure 23 tools 22 Prerequisites 6 Productreturnorservicing 39 Purposeofthismanual 7 R Recyclingin...

Страница 40: ...a Anyuseof UNICORN issubjectto Cytiva StandardSoftwareEnd UserLicenseAgreementforLifeSciencesSoftwareProducts Acopy ofthisStandardSoftwareEnd UserLicenseAgreementisavailableonrequest Allgoodsandservic...

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