7
Protein electrophoresis
Casting Unit Preparation
Cleaning the Glass Plates
Clean a set of glass plates for each gel first with distilled water and then with
70 % ethanol. One set of glass plates constitutes one notched glass plate
and one plain glass plate with bonded spacers.
When using a triple glass plate sandwich, two notched glass plates are
required, one set of free spacers and a set of plain glass plates with bonded
spacers. The plain glass plate is positioned outermost, then a notched glass
plate, free spacers and second notched glass plate. Alternatively, accessory
notch glass plates with bonded spacers are available.
Note: All glass plates, gel casting modules, casting base and accessories must be
completely dry before the set
–
up. Wet components are more likely to miss-align and
cause leaks.
Gel Cassette Assembly
Assemble the glass plates in the CVS10DRIM casting/running insert so that the
bottom of the glass plates and the spacers are perfectly aligned
on a flat
levelled surface (i.e. laboratory bench
–
outside the casting base).
A triple plate sandwich can be used by combining the standard Notched
plate and spacer plate, with an additional notched plate with spacers. For
triple plate sandwiches, the free spacers Need to be perfectly aligned which
is best performed using a small spacer or comb to push the spacers apart.
Notched glass plates with bonded spacers do not need manual alignment.
NOTE: If only one gel needs to be casted, please make sure to use the
dummy plate on the other side otherwise the casting/running
insert won’t be
straight.
Casting Stand Assembly
1.
Ensure that the pressure bars are adequately open for the thickness of
spacer used. The bar can be opened by loosening the screws or by
sliding the clamps. When using a triple glass plate sandwich, the
