Cleaver Scientific CVS10CBS, Инструкция по эксплуатации

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Problem: Electrophoresis Cause Solution
Current zero or less than
expected and samples do not
migrate into gel Tape at the bottom of precast
gel cassette not removed
Insufficient buffer in inner buffer
chamber
Insufficient buffer in outer buffer
chamber
Electrical disconnection Remove tape.
Fill buffer chamber with running buffer.
Fill inner and outer buffer chambers to ensure
wells are completely covered.
Check electrodes and connections.
Gels run faster than expected Running buffer too concentrated
and gel temperature too high;
incorrect running buffer
concentration or type used
Running or reservoir buffer too
dilute
Voltage too high Check buffer composition and type.
Check buffer protocol and concentrate if
necessary.
Decrease voltage by 25-50%.
Gels run slower than expected Incorrect running buffer
composition or type
Excessive salt in sample Check buffer composition and type.
Desalt sample.
Problem: Total Protein
Staining Cause Solution
Bands not visible No protein in gel
Imaging system malfunctioning
Incorrect imaging parameters
were used Stain with another method to confirm there is
protein.
Check instrument manual for troubleshooting
or contact imaging instrument manufacturer.
Check Instrument manual.
Poor staining sensitivity Dirty staining trays
Insufficient stain volume
Insufficient staining time
Reuse of staining solution Clean staining trays and other equipment
with laboratory glassware cleaner.
Follow recommendations for stain volume
(appropriate to gel size).
Increase staining time.
Repeat staining protocol with fresh staining
solution.
High or uneven background
staining Staining trays or equipment dirty
Too much time in staining
solution
Reagent impurities
Clean staining trays and other equipment
with laboratory glassware cleaner.
Restrict duration of incubation in staining
solutions as recommended in protocol.
Wash gel in water or retrospective destaining
solution for >30min.
Use high-purity water and reagents for
staining.
Speckles or blotches in gel
image Particulate material from
reagents, staining tray, dust or
gloves Clean staining trays thoroughly.
Decrease time that gels and staining solution
are exposed to open air.
Use dust-free gloves and handle gels only by
edges.
Uneven staining Insufficient shaking during
staining Agitate gel during staining.
Gel shrinkage Gel dehydrated Transfer gel to water for rehydration.
Problem: Evaluation of
Separation Cause Solution
Diffuse or broad bands Poor quality acrylamide or bis-
acrylamide incomplete
polymerization
Old SDS or sample buffer Use electrophoresis-grade reagents.
Check polymerization conditions.
Prepare fresh solutions.