17
2. Close the cassettes hinge carefully to not disturb the sandwich.
3. Fill the tank with buffer solution up to the maximum fill line indicated on
the side of each unit. See recommended buffer solutions section.
NOTE: Improved transfer can usually be obtained by using chilled buffer.
Buffer Volume
SB10
One Cassette
Two Cassettes
Three Cassettes
Four Cassettes
1380ml
1290ml
1200ml
1110ml
Each Cooling pack will take the place of ~ 100ml of buffer
Blot Running Conditions
1.
Insert the cassettes into the slots in the VS10BI blotting module with the
black side of each adjacent to the negative electrode. It is a good
idea to note the orientation and order the blot sandwiches were
loaded in. This can be done by noting which samples were loaded
adjacent to each electrode and by marking the membrane with a
pencil on one corner.
2.
Use of a magnetic stirring bar and plate is recommended to mix the
buffer to give consistency of transfer. A 4mm diameter stirring bar
should be placed underneath the module, in the centre of the tank.
The Cooling pack provided, pre-frozen, can be inserted at the side or
front of the tank for extended blots. Additional cooling packs can be
purchased as accessories to further aid cooling.
3.
Insert the blotting module in the tank making sure the polarity is correct,
fit the lid and connect to a power supply.
4.
Consult Suggested Running Condition for recommended power supply
voltage settings and blot times. Please note voltages and current will
