C.B.S. SCIENTIFIC EBU-4000 Скачать руководство пользователя страница 14

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C.B.S.

Scientific Co, Inc.

Semi-Dry

Blotters

SECTION 6
Equipment and Accessories

Cat. #

Item

EBU-4000

Semi-Dry Blotting System

. Includes power leads and safety interlock, 20cm x 20cm.

EBU-6000

Semi-Dry Blotting System

. Includes power leads and safety interlock, 35cm x 45cm.

MM-4000

Mylar® Mask for EBU-4000, package of 4 each

MM-6000

Mylar® Mask for EBU-6000, package of 6 each

Содержание EBU-4000

Страница 1: ...Semi Dry Blotting Systems INSTRUCTION MANUAL EBU 4000 EBU 6000...

Страница 2: ...ction 5 1 2 Specifications 5 1 3 Safety 5 Section 2 Description of parts 2 1 Unpacking EBU 4000 or 6000 6 2 2 Components Assembly 6 Section 3 Instructions for Use 3 1 Blotting Unit Preparation 7 11 Se...

Страница 3: ...e highest practical standards of materials workmanship and design C B S Scientific warrants that the product has been tested and will meet or exceed published specifications This warranty is valid onl...

Страница 4: ...endimiento insatisfactorio atribuible a circunstancias fuera del control de C B S Scientific C B S Scientific en ning n caso asumir responsabilidad por da os incidentales o subsecuentes incluyendo en...

Страница 5: ...t passes directly through the gel Use of the mask will increase transfer efficiency and reduce transfer times up to 50 1 2 Specifications Constructions Buffer chamber safety cover Acrylic Electrode pa...

Страница 6: ...nts Blotting chamber with stainless steel cathode and platinum coated anode Safety cover with power leads Mylar sheets for shielding uncovered portion of anode 2 2 Components Assembly SAFETY COVER POW...

Страница 7: ...is why nylon membranes are generally used uncharged nylon being preferable to positively charged nylon Nylon is the membrane of choice because it will bind DNA fragments as small as 50bp and can be ir...

Страница 8: ...g on thickness 3 6mm c Cut eight sheets of 3mm blotter paper Whatman the exact size of the gel Saturate all eight sheets in running buffer d Mylar Mask Prepare a mask by cutting a rectangular hole tha...

Страница 9: ...ng a rectangular hole that is slightly smaller 1 2mm than the gel dimensions b Prepare 8 pieces of Whatman 3mm chromatography paper the exact size of the gel Larger pieces of filter paper may promote...

Страница 10: ...At the conclusion of the run turn power supply off and disconnect leads from blotter Cover will not release unless power cord is removed Remove the cover slowly to catch any part of the gel sandwich...

Страница 11: ...lowed by the gel and 5 more pieces of saturated filter paper Again roll a glass rod over the filter paper stack to compress and remove trapped air bubbles The lid of the apparatus containing the catho...

Страница 12: ...sis 86 315 327 VCH Weinheim Germany 3 Dunn S D Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by mo...

Страница 13: ...lpyrocarbonate DEPC treated water for extended periods at 37 C Electrostatic charge and heat affix the conductive platinum surface covering the titanium It can be scratched off or damaged by using sha...

Страница 14: ...t Item EBU 4000 Semi Dry Blotting System Includes power leads and safety interlock 20cm x 20cm EBU 6000 Semi Dry Blotting System Includes power leads and safety interlock 35cm x 45cm MM 4000 Mylar Mas...

Страница 15: ...NOTES...

Страница 16: ...d online ordering www cbsscientific com E mail address sales cbssci com Mailing address C B S Scientific Company P O Box 856 Del Mar CA 92014 Shipping address C B S Scientific Company 10805 Vista Sorr...