Buchi Pure C-805, Руководство по эксплуатации

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BÜCHI Labortechnik AG Appendix | 10
Operation Manual Pure Chromatography Instrument C-805 67/70
u Adjust the following values if necessary:
—
Column
— Flow rate
— Speed / Purity
u Tap the button [Accept] .
ð The gradient is saved for a run.
ð The dialog box closes.
10.3.3 LC-C18
The LC-C18 Tool uses isocratic HPLC separation data to provide a recommended
gradient for reversed phase separations of 2 components.
Specification of the samples needed:
— Two isocratic runs on a HPLC column
— Different mobile phase solvent concentrations
—
Retention times (t1 and t2) of the components from the chromatograms.
Precondition:
R
The display shows the dialog Navigator . See Chapter 10.3.1 "Open the naviga-
tor", page 65
u Select the LC-C18 tab.
u Select the HPLC column used from the drop-down list.
u Select the weaker solvent at drop-down list A.
u Select the stronger solvent at drop-down list B.
u
Enter the solvent concentrations used in the HPLC separations.
u
Enter the retention times for each separation under each chromatograph.
u
Select the column you wish to use from the drop-down list
ð
The column is selected.
ð
The display shows the default flow rate.