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6 | Operation

BÜCHI Labortechnik AG

42/70

Operation Manual Pure Chromatography Instrument C-805

6.4

Tasks during a separation

6.4.1

Injecting a sample into the flash system

Injecting a sample into the cartridge

u

Remove the solvent line from the cartridge.

u

Connect the syringe to the cartridge.

u

Press the plunger.

u

Disconnect the syringe.

Содержание Pure C-805

Страница 1: ...Pure Chromatography Instrument C 805 Operation Manual 11594236 B en...

Страница 2: ...ves the right to make changes to the manual as deemed necessary in the light of experi ence especially with respect to structure illustrations and technical details This manual is copyrighted Informat...

Страница 3: ...ent 9 2 5 Personal protective equipment 9 2 6 Modifications 9 3 Product description 10 3 1 Description of function 10 3 2 Configuration 11 3 2 1 Front view 11 3 2 2 Rear view 11 3 2 3 Connections on g...

Страница 4: ...stem 42 6 4 2 Installing and removing a cartridge 43 6 4 3 Inserting the fraction collection tray 44 6 4 4 Selecting an existing method 44 6 4 5 Selecting values on the dialog box Sample Loading 45 6...

Страница 5: ...sible causes and solutions detection 57 8 6 Error messages 57 8 7 Resetting the Fraction collector arm 61 9 Taking out of service and disposal 62 9 1 Taking out of service 62 9 2 Disposal 62 9 3 Retur...

Страница 6: ...this document Warning notices warn you of dangers that can occur when handling the device There are four danger levels each identifiable by the signal word used Signal word Meaning DANGER Indicates a...

Страница 7: ...s Tab Tabs are marked up like this Dialog Dialogs are marked up like this Button Buttons are marked up like this Field names Field names are marked up like this Menu Menu item Menus or menu items are...

Страница 8: ...e operating instructions are aimed at the following target groups Users Users are persons that meet the following criteria They have been instructed in the use of the device They are familiar with the...

Страница 9: ...ntivirus software and firewall on the instrument before connecting it to other devices or network 2 4 3 Damage to the internal memory due to incorrect shutting down of the instrument Incorrect shuttin...

Страница 10: ...mplex samples by flash chromatography Flash chromatography has the ability to separate gram size samples in short pe riod The instrument allows 2 different solvents Injection of liquid or solid sample...

Страница 11: ...hy Instrument C 805 11 70 3 2 Configuration 3 2 1 Front view 5 7 1 2 3 4 6 Fig 1 Front view 1 Control panel 2 RFID reader 3 On Off switch 4 USB Port 5 Cartridge holder 6 Flash pump 7 Fraction collecti...

Страница 12: ...805 9 8 7 6 1 2 3 4 5 Fig 2 Rear view 1 Ventilation slot 2 Gradient valve See Chapter 3 2 3 Connections on gradient valve page 13 3 Signal connection for external air supply 4 LAN port 5 Power supply...

Страница 13: ...he instrument The type plate is located at the rear of the in strument Buchi Labortechnik AG 9230 Flawil Switzerland Type xxx SN 10xxxxxxxx Volt xxx xxx VAC Frequ xx Hz Power xxxx W Built 20xx Made in...

Страница 14: ...D x H 365 x 570 x 680 mm Weight 24 kg Clearance on each side of the instrument 200 mm Power consumption 350 W Connection voltage 100 240 V 10 Frequency 50 60 Hz Fuse 4 A Overvoltage category II Pollut...

Страница 15: ...e sea level 2000 m Ambient temperature 5 40 C 25 C No maximum performance above 25 C Maximum relative humidity 80 for temperatures up to 31 C Storage temperature max 45 C 3 5 3 Material Component Mate...

Страница 16: ...d sharp movements during transit u After transportation check the device for damage u Damage that has occurred in transit should be reported to the carrier u Keep packing for future transportation 4 2...

Страница 17: ...ger due to incorrect transportation The possible consequences are crushing injuries cuts and breakages u The instrument should be transported by two persons at the same time u Lift the instrument at t...

Страница 18: ...bes can be routed safely The installation site allows that the power supply can be disconnected at any time in an emergency The installation site is not exposed to increased electromagnetic emissions...

Страница 19: ...to use of un suitable power cables The power supply cables supplied with the product by BUCHI precisely match the re quirements of the device If other power cables that do not meet those requirements...

Страница 20: ...connected to the instrument See Establishing solvent con nection R The solvent you wish to use is part of the solvent library See Chapter 6 3 Editing a solvent page 40 u Navigate to the Solvent Loadi...

Страница 21: ...perty damages u Make sure that there is a decline between the connection on the instrument and the waste bottle u Put the waste line into the waste bottle 5 8 Assembling the solvent bottle platform op...

Страница 22: ...70 Operation Manual Pure Chromatography Instrument C 805 u Attach the drain line adapter to the solvent bottle platform u Attach the drain line to the drain line adapter u Attach the solvent bottle p...

Страница 23: ...operties and default set tings of the installed column cartridge See Conditions panel 3 Wavelength panel Shows available wavelengths and scan options 4 Collection panel Shows collection options See Ch...

Страница 24: ...ws selectable slope detection op tions See Chapter 6 1 10 Slope detection panel page 32 6 1 2 Enter value Enter numbers u Tab on an entry field The display shows a dialog box with a numeric input box...

Страница 25: ...ve Method as Save an edited method with another name See Chapter 6 2 8 Saving a method page 40 Open Run Load a completed run Print Run Report See Chapter 6 8 1 Printing a run report page 49 Print PDF...

Страница 26: ...ity page 39 Configuration System configurations Calibration and Defaults Calibrate the screen Set time and date Reset UV lamp Set alarms Manual control Maintenance actions See Chapter 7 5 Cleaning the...

Страница 27: ...led in the instrument Flow Rate Enter value Edit the default flow rate Duration Units Choice of the duration type The following types are available minutes column volumes Equilibration Enter value Ind...

Страница 28: ...he estimated number of vials required for the separation is displayed below the solvent usage list in the lower left corner of the Setup window The fraction collection area contains the following sett...

Страница 29: ...tions See Chapter 10 3 Finding out sepa ration conditions with the navigator page 65 UV scan details View Shows the following charts 3D UV Time Wavelength 2D Time Wavelength Absorption maxima All scan...

Страница 30: ...nel The run panel shows available functions according to the current operation Symbol Description Meaning Start Is used to start the run or re start the actual operation if the system has been paused...

Страница 31: ...tion See Chapter 6 2 2 Selecting a solvent page 33 6 1 9 Detector selection panel Action Option Explanation UV Enable Disable Select value Enter value Action enabled Low High Enter values for threshol...

Страница 32: ...Slope detection Enable Disable Action enabled Fraction collection triggered by slope Action disabled No detection 6 2 Editing a method 6 2 1 Selecting a cartridge Flash mode NOTE The automatic readin...

Страница 33: ...n button Information about the selected col umn Navigation path Conditions panel u Navigate to the Conditions selection panel according the navigation path u Tap the input box next to Column The displ...

Страница 34: ...e navigation path u Tap the drop down list next to A The display shows a drop down list with the assigned solvents u Tap the required solvent The solvent is selected The drop down list closes u Select...

Страница 35: ...isplay shows a dialog box with a numeric input box u Enter the percentage for the second solvent u Tap the button OK u Tap the button Close The gradient is saved The dialog Gradient table closes The G...

Страница 36: ...ctor Status Checkbox Explanation Explanation On The instrument collects fractions Monitoring The instrument records the data from the UV detector but does not collect the frac tions Off The instrument...

Страница 37: ...of de tection signals Collect None The instrument collects no fractions NOTE Peak and Non Peaks default is the maximum volume of the vial Navigation path Collection panel u Tap the radio button next...

Страница 38: ...The fraction collector defaults to collect the full run length unless the values from Pro gram Collect override the collection Navigation path Gradient panel u Navigate to the Gradient panel accordin...

Страница 39: ...is deleted 6 2 7 Editing the vapor sensor sensitivity The vapor sensor detects solvent concentration in the ambient air The following sensitivity limits are available Sensitivity Meaning High Used fo...

Страница 40: ...er method name The display shows a dialog with an alphanumeric input box u Enter the name of the method u Tap the button OK The dialog box closes u Tap the button OK The method is saved The dialog box...

Страница 41: ...he button OK The dialog box closes u Tap the button Verify Solvents The display shows the dialog box Solvent Verification u Select solvent group for the defined solvent u Tap the button OK The dialog...

Страница 42: ...ography Instrument C 805 6 4 Tasks during a separation 6 4 1 Injecting a sample into the flash system Injecting a sample into the cartridge u Remove the solvent line from the cartridge u Connect the s...

Страница 43: ...d of a cartridge a bypass can be installed NOTE The automatic reading of the cartridge information can only be done with specific BUCHI RFID tagged cartridges 4 The indication for recognizing the cart...

Страница 44: ...Loading page 45 1 2 1 Tab 2 Optical sensor slot u Place the collection tubes in the collection tray u Place the trays in the fraction collector bay u Make sure that the tab is in the optical sensor s...

Страница 45: ...ee Chapter 5 Installation page 18 u Switch the On Off master switch to On u Tap the On Off switch The instrument is starting up 6 5 2 Starting Flash separation using a method Precondition R The instru...

Страница 46: ...line at the end of the separation is not below the set threshold Precondition R The display shows the dialog box Separation End u According to the requirements extend the separation time by 5 minutes...

Страница 47: ...solvent is selected The drop down list closes u Select more solvents for the mobile phase according to your needs u Edit the gradient according to your needs See Chapter 6 2 3 Editing the gradi ent p...

Страница 48: ...ndition R The display shows the dialog box Run completed u Follow the instructions on the display Removing the cartridge See Installing and removing a cartridge 6 6 5 Shutting down the instrument Navi...

Страница 49: ...fying fractions per vial Fig 8 Identifying fractions Navigation path Collection panel Precondition R A separation is finished u Navigate to the Collection panel according the navigation path u Tap the...

Страница 50: ...e Run panel according the navigation path u Tap the button Data to USB The instrument saves an Excel file to the USB storage device u Confirm the complete message The data is stored 6 8 3 Sending PDF...

Страница 51: ...fittings if solid material is de posited on a fitting clean and tighten re place the fitting before continuing Daily Drain lines u Check all drain lines to ensure that liquid can flow through them to...

Страница 52: ...the Windows Explorer u Navigate to the data you wish to backup See Chapter 10 2 Folder locations page 65 u Copy the needed data to the USB storage devices 7 3 Calibrating the display Navigation path T...

Страница 53: ...Off u Disconnect the mobile phase tubing from the inlet bottom of the pump head us ing the 9 16 open end wrench u Disconnect the mobile phase outlet tubing from the top of the pump head using the 5 16...

Страница 54: ...AG 54 70 Operation Manual Pure Chromatography Instrument C 805 u Install a bypass See Installing and removing a column u Navigate to the Column Flushing dialog according to the navigation path u Ente...

Страница 55: ...cal ibration u Recalibrate the touch screen 8 2 Faults possible causes and solutions cartridge Malfunction Possible cause Solution Cartridge is not de tected RFID tag is not facing the RFID reader u T...

Страница 56: ...replace it Over tightened fitting u Loosen the fitting or re place it Blocked columns or fluidic path u Locate the component that caused the blockage re pair or replace the compo nent Leaks Fitting c...

Страница 57: ...ause Solution No UV signal UV light burned out u Replace the UV light Low UV signal Dirty flow cell u Clean the flow cell 8 6 Error messages Error message Possible cause Solution Instrument Alarm Solv...

Страница 58: ...e tector analog or digital voltage Hardware error UV detector u Contact BUCHI Customer Service Instrument Alarm UV detector 4th cycle of lamp igni tion fails Lamp defect u Check the lamp and restart i...

Страница 59: ...is not working or lamp spontaneously douse 2nd lamp defect u Check the lamp and restart instrument Instrument Alarm UV detector Any fan is not working or any fan is dis connected or me chanical block...

Страница 60: ...the calibration list u Restart the Pure software Sensor pressure calibration file Pressure sensor calibration file damaged u Shutdown the Pure soft ware u Delete the pressure value list u Restart the...

Страница 61: ...ion col lector arm page 61 Fraction collector arm did not home properly u Tighten the motor coupler u Contact BUCHI Customer Service Fraction collector arm motor is slipping u Contact BUCHI Customer S...

Страница 62: ...tion cables from the device 9 2 Disposal The operator is responsible for proper disposal of the instrument u When disposing of equipment observe the local regulations and statutory require ments regar...

Страница 63: ...s Order no Image Pure rack type 1 Rack for 12 x 75 mm glass tubes 1 pc 11066672 Pure rack type 2 Rack for 13 x 100 mm glass tubes 1 pc 11066673 Pure rack type 3 Rack for 16 x 125 mm glass tubes 1 pc 1...

Страница 64: ...Touch screen stylus 11068360 Hook and loop strap 11071732 10 1 3 Tools and adapter kits Order no Luer lock connection set Set of 2 luer lock adapters 11068242 Advanced adapter kit flash Tube cutter Fi...

Страница 65: ...settings the following folder locations are hidden 4 Start the software Windows Explorer on the instrument 4 Navigate to folder options via the following navigation path View Folder Op tions View 4 Ac...

Страница 66: ...lect the radio button for two or three components u Select the weaker solvent at drop down list A u Select the stronger solvent at drop down list B u Enter the solvent B concentrations used in the TLC...

Страница 67: ...column Different mobile phase solvent concentrations Retention times t1 and t2 of the components from the chromatograms Precondition R The display shows the dialog Navigator See Chapter 10 3 1 Open th...

Страница 68: ...graphy gradi ent Specification of the sample needed One run on a HPLC column in gradient elution mode Times B changes t1 t2 and t3 Precondition R The display shows the dialog Navigator See Chapter 10...

Страница 69: ...graphy Instrument C 805 69 70 u Adjust the flow rate according to your needs u Tap the button Calculate The display shows the results u Adjust the following values if necessary Flow rate u Tap the but...

Страница 70: ...n zh India BUCHI India Private Ltd IN Mumbai 400 055 T 91 22 667 75400 F 91 22 667 18986 india buchi com www buchi com in en Indonesia PT BUCHI Indonesia ID Tangerang 15321 T 62 21 537 62 16 F 62 21 5...

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