BÜCHI Labortechnik AG
Operation | 6
Operation Manual Pure Chromatography Instrument C-805
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u
Switch the
On/Off
master switch to On.
u
Tap the
On/Off
switch.
ð
The instrument is starting up.
6.6.2
Starting Flash separation manually
Precondition:
R
The instrument is prepared. See Chapter 6.6.1 "Preparing the instrument",
page 46
R
R
The sample is prepared.
R
The waste bottle is empty.
u
Open the protection shield.
u
u
Close the protection shield.
u
Select a cartridge. See Chapter 6.2.1 "Selecting a cartridge (Flash mode)",
page 32
u
Tap the drop-down list next to A: on the
Solvent Selection
panel.
ð
The display shows a drop-down list with the assigned solvents.
u
Tap the required solvent.
ð
The solvent is selected
ð
The drop-down list closes.
u
Select more solvents for the mobile phase according to your needs.
u
Edit the gradient according to your needs. See Chapter 6.2.3 "Editing the gradi-
ent", page 34
u
Select the sample collection in the
Collection
panel. See Chapter 6.2.5 "Editing
the fraction collection criteria", page 37
u
Select the collection criteria in the
Collection criteria
panel.
u
Tap the button
[Start]
on the
Run
panel.
ð
The display shows the dialog box
Sample Loading
.
u
According the requirements adjust the settings. See Chapter 6.4.5 "Selecting val-
ues on the dialog box Sample Loading", page 45
u
Follow the instructions on the display.
ð
Installing the cartridge. See Installing and Removing a cartridge
ð
Introducing the sample into the system. See Chapter 6.4.1 "Injecting a sample
into the flash system", page 42
6.6.3
Changings during a separation
NOTE
Parameters that can be edited are highlighted in green
Possibilities to edit the gradient during a separation:
Change the gradient. See Editing the gradient in graphic mode
Hold the gradient. See Gradient panel
Auto gradient hold. See Gradient panel
