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BRIGHT OTF 5000, Инструкция по эксплуатации

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OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012  

41 

 

7 

PRODUCT INFORMATION 

 

Introduction 

 
Based on the long-established and reliable OTF/AS cryostat, the new OTF5000 brings Bright 
cryostats completely up to date. New styling with improved user ergonomics, the latest blade 
systems in the ever-reliable and powerful 5040 microtome, a huge choice of options plus 
money-saving package deals make these cryostats absolutely unique. 
 
They are suitable for an endless range of applications and in the correct configuration are 
capable of cutting a wide diversity of specimens from undecalcified bone to brain, resins, 
plastics and plant tissue as well as more usual soft tissue. Allowing full anti-roll plate adjustment 
for perfect results coupled with long lasting temperature stability, the OTF5000 can truly be 
described as complete. 
 

SPECIAL FEATURES: 

 

•

  New contoured top for added operator comfort and usability 

•

  Suitable for research and clinical operation 

•

  2 money-saving special packages available  

•

  Uses solid knives or disposable blades including new lever release 

Feather

TM

 blade holder 

•

  Available in 2 different working heights 

•

  Automatic evaporator defrost supplied as standard 

•

  Non-urgent functions on side panel 

•

  Long list of options and accessories 

•

  Contains tried and tested 5040 rotary microtome  

 

Configurations 

 
The OTF5000 cryostats are available in 3 configurations: 
 
Basic Instrument which includes; Quick Freezer, Internal shelves, Evaporator defrost, Manual 
cutting system, 5040 microtome with fixed head, Heated window surround, Window demist 
system, Analogue defrost clock, Side mounted non urgent controls and a full set of accessories. 
 
LS specification, which includes all of the features of the basic instrument and additionally; 2 
speed motorised advance/rewind, Fine object orientation and Cryomatic specimen temperature 
control. 
 
HS specification, which includes all the features of the LS specification and additionally; 6 digit 
section counter, Dual refrigeration and Motor drive. 
 

Basic Specification 

 
Cryochamber 

Polished stainless steel construction 

Quick freezer 

9 position, running at 10º C below chamber 
temperature, ultra low temperature 

–80ºC 

version can be factory fitted at extra cost  

Minimum chamber temperature 

Single compressor 

–35ºC 

Shelves 

3 internal, 1 external 

Chamber temperature control 

Microprocessor with LED display 

Evaporator defrost 

Automatic with analogue clock 

Cutting system 

Manual with balanced handwheel 

Microtome  

5040 rotary microtome with beryllium hinges 

Содержание OTF 5000

Страница 1: ...strial Estate Huntingdon Cambridgeshire England PE29 6EU Tel 44 0 1480 454528 451980 451499 Fax 44 0 1480 456031 Email sales brightinstruments com Website www brightinstruments com INSTRUCTION MANUAL...

Страница 2: ......

Страница 3: ...STALLATION OF TRAYS AND SHELVES FIG 2 4 1 2 4 FROST ELIMINATION COVER FIG 3 5 1 2 5 INSTALLATION OF THE MICROTOME and REMOVAL 5 1 2 6 INSULATED NIGHT PLUG 6 1 2 7 X THIN SECTION CONVERSION KIT where a...

Страница 4: ...E BLOCK 16 2 2 1 KNIFE GUARDS 16 2 2 2 KNIFE HOLDER 17 2 2 3 X THIN SECTION CONVERSION KIT where applicable 17 2 2 4 ANTI ROLL ASSEMBLY 18 2 3 MICROTOME CONTROLS Quick Release Feather Blade Holder 20...

Страница 5: ...USES 33 4 7 2 CHANGING FLUORESCENT TUBE 33 4 8 DECONTAMINATION 33 5 ACCESSORIES 34 5 1 OBJECT HOLDERS 34 5 2 KNIVES 36 5 3 LEVER RELEASE FEATHER BLADE HOLDER 37 5 4 MAGNAPLATE ANTI ROLL PLATE 37 5 5 M...

Страница 6: ...Specification 42 HS Specification 42 Appendices 5040 MICROTOME DRAWINGS relevant to Model 5040 MICROTOME PART LIST relevant to Model ELECTRICAL CIRCUIT DIAGRAMS relevant to Model Material Safety Shee...

Страница 7: ...results b Fuses Fuse ratings are clearly indicated on all fuse panels adjacent to the fuse holder If and when replacement is necessary the correct fuse rating must be adhered to c Earthing Grounding...

Страница 8: ...t of it is to be returned to Bright Instrument Co Ltd please note the following 1 If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive ma...

Страница 9: ...gence or otherwise in respect of defects in goods delivered or for any injury damage or loss resulting from such defects and the Seller s liability under this clause shall be in lieu of any warranty o...

Страница 10: ...ress of moist laboratory air The drain plug should be left in position except when cleaning is carried out 1 2 3 INSTALLATION OF TRAYS AND SHELVES FIG 2 A pair of stainless steel trays is packed separ...

Страница 11: ...icrotome to locate the front two studs the rear ones will follow 3 Loosen the two recessed grub screws in the coupling on the Drive shaft projecting from the right chamber wall 4 Slide the coupling on...

Страница 12: ...2 Remove Advance Rewind Knob on top of microtome by pulling upward 3 Remove LH tray by sliding forward and up 4 If a heated v block is fitted then lift it move it out of the way 5 Remove Coupling by l...

Страница 13: ...st eight hours and preferably overnight before switching on Moving the instrument around on its castors e g from one laboratory to another will not affect the compressor oil 1 3 2 ELECTRICAL REQUIREME...

Страница 14: ...with long lasting temperature stability the OTF5000 can truly be described as complete FIG 1 SWITCHING ON On receipt of your new Bright instrument please refer to the Receipt and Unpacking section and...

Страница 15: ...clear the evaporator cooling fins without the rest of the chamber rising above 0 C Set the 12 hour analogue clock to the current time by turning the clear plastic disc with your finger Note that beca...

Страница 16: ...than the set chamber temperature or if the chamber temperature goes 20 C colder than the set temperature The Alarm can be manually reset by pressing any one of the 3 Controller buttons on the Chamber...

Страница 17: ...ination cover after using the quick freezer plate this helps prevent a build up of frost 2 1 8 QF SPECIMEN QUICK FREEZER where applicable FIG 10 The specimen quick freezer QF is designed for rapid fre...

Страница 18: ...0 C If the specimen is then placed in the vice jaws it will be far too cold to cut and if attempted damage may occur to knives It is imperative that a little time is allowed for the specimen temperatu...

Страница 19: ...andwheel The longer the switch is pressed the thicker the section If the Advance switch is pressed for more than 1 5 seconds a faster advance of the specimen is achieved Motorised Specimen Rewind 1 To...

Страница 20: ...ions can be used at any time Pull out the connecting rod knob to operate the microtome manually The Emergency Stop button stays in when pressed Twist button in direction of arrows to release Then pres...

Страница 21: ...ain active To prevent airborne contamination in the chamber due to convection air currents caused by the refrigeration system it is recommended that the UV system is switched on at all times the cryos...

Страница 22: ...e guard 19 Anti roll extension knob 10 Knife guard release knob 2 2 1 KNIFE GUARDS A pair of knife guards 9 are fitted and it is strongly recommended that they remain fitted at all times When access t...

Страница 23: ...guards to the centre and slide the knife out through to the left 2 2 3 X THIN SECTION CONVERSION KIT where applicable This kit consists of a 0 2 12 m feed screw part number 50256 an orientating glass...

Страница 24: ...ion 3 2 3 Release the knife block clamp locking lever 3 and turn the knife carriage feed control 4 clockwise to bring the knife towards the specimen 4 Trim the specimen or embedding medium until a sui...

Страница 25: ...to cut sections again They will curl up over the roll plate 5 Slowly turn the anti roll micro adjusting knob 17 clockwise while cutting sections When the plate 15 reaches the correct height the secti...

Страница 26: ...Roll Plate Adjuster 3 Blade Angle Indicator 11 Stem Clamp Knob 4 Wire Knife Guard 12 Blade 5 Blade Loading Plate 13 Anti Roll Plate Clamp Knob 6 Fixed Blade Guard 14 T Piece Clamp Knob 7 Easi Set Ant...

Страница 27: ...towards it To install a blade Feather blades are covered with a fine film of protecting varnish It is necessary to remove this varnish using solvents i e xylene or acetone before using a blade for sec...

Страница 28: ...leading edge of the anti roll plate is parallel to the edge of the blade b the leading edge of the anti roll plate is parallel in line with the blade c the under side of the anti roll plate is at an a...

Страница 29: ...ed control 2 4 2 SECTION THICKNESS CONTROL See above To select section thickness set the thickness control 1 at zero and rotate anticlockwise until desired thickness is level with the indicator To res...

Страница 30: ...AR 50258 manual 50258 1 MAR 50259 manual 50259 1 MAR 50260 manual Thin Section Standard in cryostat Standard in wax microtome 0 2 to 12 m 0 5 to 30 m 1 to 60 m 2 to 120 m 3 to 180 m 5 to 300 m 0 2 m i...

Страница 31: ...l rule the quicker tissues are frozen the better the results will be The aim is to minimise the damage caused by ice crystal artefact and the more rapid the transition from liquid phase to solid phase...

Страница 32: ...freezing media 3 1 5 BRIGHT CLINI RF The Bright Clini RF is a small free standing top opening ultra low temperature freezer Its function is to rapidly freeze tissue specimens ready for cryotomy or for...

Страница 33: ...der from the quick freezer and place it in the V block or cryomatic block and tighten the screw firmly to hold it in position Wait a few minutes for temperatures to equalise before trimming It is ofte...

Страница 34: ...t sections if adjustments to the anti roll plate are required see section 2 2 8 4 To collect sections have a clean microscope slide ready at ambient temperature Bring the slide towards the knife swing...

Страница 35: ...of factors The notes which follow deal with the more commonly encountered difficulties and suggest a systematic approach to problem solving 3 6 1 SOLUTIONS CHECK LIST First check all the basic factor...

Страница 36: ...d debris always brush up the knife never down or along Sections curl over anti roll plate anti roll plate too low Section curl under anti roll plate anti roll plate at too large an angle to knife Sect...

Страница 37: ...tion 2 2 2 and clean it see section 4 4 4 Remove the microtome from the chamber the reverse of the installation procedure see section 1 2 5 Remove drain plug from base of chamber 5 Place the microtome...

Страница 38: ...until the arrival of the engineer Failure to carry out this action will result in the service visit being cancelled and could incur further call out charges A completed decontamination certificate mus...

Страница 39: ...use holder 6 Reconnect the power supply and ensure instrument is working correctly If there are any further problems contact your local distributor or Bright Instrument Co Ltd direct 4 7 2 CHANGING FL...

Страница 40: ...ct holder 22mm long type gives greater clearance between specimen clamp and knife holder Ideal for when multi position glass slides are used 50735 1 Object holder with stem 37mm dia Suitable for large...

Страница 41: ...ect holder discs 50mm dia pack of 100 Orientating specimen holders 51695 Universal holder This simple inexpensive device will fit 5030 or 5040 microtomes that have fixed heads Any of the object holder...

Страница 42: ...older for Shandon knife sharpener safety cut outs at each end 50232 1 Hardened steel knife supplied in box C profile 160 x 35 x 9mm 15 angle suitable for large area sections 50207 Similar to 50232 1 b...

Страница 43: ...will Fit most knives and blades It is shown sitting on a 50230 standard knife The Magnaplate is supplied in a pack of 3 with a single removable handle 5 5 MAGNACUT MAGNETIC DISPOSABLE BLADE SYSTEM 50...

Страница 44: ...SCREWS 5030 and 5040 microtomes are fitted with a feed screw that controls the section thickness range These feed screws may be freely changed so that a new section thickness range can be given to th...

Страница 45: ...6 bottles Bright Cryo M Bed is a blend of polyviol alcohol 217 thymol and water It is an inert colourless viscous fluid which is totally transparent at room temperature It becomes opaque when frozen a...

Страница 46: ...fe with box 228 x 38 x 6mm Suitable for cutting bone and hard plastics NB needs to be resharpened at our factory 52647 Stem for 50232 1 and 50234 knives 52671 Knife sharpening kit Includes hone strop...

Страница 47: ...defrost supplied as standard Non urgent functions on side panel Long list of options and accessories Contains tried and tested 5040 rotary microtome Configurations The OTF5000 cryostats are available...

Страница 48: ...gotiate doorways a further 65mm can be gained LS Specification As the basic specification but with the addition of the following items Object orientation Fine with 8 in horizontal and vertical axes 36...

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