BRIGHT OTF 5000, Инструкция по эксплуатации

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OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012
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2.2.2 KNIFE HOLDER
To ensure optimum sectioning performance the knife must be kept clean from grease and dirt.
To clean the knife, carefully apply a small amount of methylated spirits or ethanol to the surface
of the knife using a clean, dry, soft paper towel. Always move the towel away from the edge of
the knife - never towards it.
To install a knife, loosen the knife clamp screws (5), slide the knife guards to the centre and
slide the knife in from the left.
Before re-tightening the knife clamp screws, set the knife tilt angle scale (7) by adjusting the
knife angle adjustment knob (8). The correct setting of the knife angle is essential for obtaining
good results.
See the table below for a list of recommended knife angles.
Recommended Knife Angles
Angle +/-
50230 - Standard knife 15º 2.5º
50232-1 - Hardened Steel knife 12º 2.5º
50207 - Steel knife 15º 2.5º
50234 - Tungsten Carbide Tipped knife 25º 2.5º
Lever release Feather blade holder 7.5º 2.5º
To remove a knife, loosen the knife clamp screws (5), slide the knife guards to the centre and
slide the knife out through to the left.
2.2.3 /X - THIN SECTION CONVERSION KIT (where applicable)
This kit consists of a 0.2 - 12µm feed screw (part number 50256), an orientating glass knife
holder (part number 50681) and a 0.2 - 12µm section thickness scale (part number 52364). The
thin section conversion kit should only be fitted with the cryostat fully defrosted,
decontaminated, clean, dry and switched off. For details of a total defrost refer to section 4.3.
Step 1 - Fit the 0.2 - 12µm Feed Screw
(refer to section Error! Reference source not
found. ).
Step 2 - Fitting the Orientating Glass Knife Holder
Refer to Figure 16
1 Loosen the knife clamp screws (5) and lift the knife guards up.
2 Place the orientating glass knife holder in from the top.
3 Set the knife tilt angle scale (7) to the desired angle. Usually between 1º and 3º maximum for
glass knives.
4 Lower the knife guards and retighten the knife clamp screws (5).
5 Place the glass knife into the orientating glass knife holder. The orientating glass knife holder
accepts 25mm high Latta-Hartman knives with edges up to 15mm.
Step 3 - Fitting the 0.2 - 12µm Section Thickness Scale
Refer to Figure 16
1 With the thickness set to zero, pull off the standard section thickness scale (19).