OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012
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2 Snap on the 0.2 - 12µm section thickness scale and the kit is ready to use.
Proceed with the sectioning of your specimens, see section 3 (Cutting and Collecting
Specimens). To remove the thin section conversion kit, just follow the instructions above
replacing items from the thin section conversion kit with the items that were removed.
2.2.4 ANTI-ROLL ASSEMBLY
Refer to Figure 16
The Easi-Set anti-roll plate (ARP) is a device for ensuring
that tissue sections pass down the knife face without
curling, so that they can be collected flat on to microscope
slides.
The design and setting up of the ARP is second only in
importance to the quality of the knife edge in obtaining
ribbons of high quality sections.
Step 1
– Preparation
1 Install a sharp Bright microtome knife (6), setting the appropriate clearance angle for that
particular knife.
2 Clamp a frozen specimen (or embedding compound alone) into the microtome (refer to
section 3.2)
3 Release the knife block clamp locking lever (3) and turn the knife carriage feed control (4)
clockwise to bring the knife towards the specimen.
4 Trim the specimen or embedding medium until a suitable block face is made (refer to section
3.3).
5 Lock the knife carriage clamp firmly and proceed to cut sections at the chosen thickness.
Once it can be seen that regular, good quality sections are cut (albeit curled up) go on to
step 2.
Step 2 - Setting the Anti-Roll Plate
Refer to FIG 16
To ensure optimum sectioning performance the anti-roll plate must be kept clean from grease
and dirt. To clean the anti-roll plate carefully apply a small amount of methylated spirits or
ethanol to both the upper and lower surfaces using a clean, dry, soft paper towel. Take care not
to break the side panels which are quite delicate.
It is important that the anti-roll plate is adjusted so that:
a)
the leading edge of the anti-roll plate is parallel to the edge of the knife
b)
the leading edge of the anti-roll plate is parallel in line with the knife
c )
the under side of the anti-roll plate is at an angle slightly greater than parallel with
the facet of the edge of the knife
Содержание OTF 5000
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