4
TurboVap
®
96 Dual User Manual | © Biotage 2021
System Overview
Evaporation Process
During an evaporation run, heated gas flows through the
gas nozzles into each well, heats up the sample in the well
and causes the solvent to evaporate. The solvent vapors are
extracted from the well by the gas flow, and by the negative
pressure applied through the system exhaust fan, see Figure 3.
Simultaneously, the plate heaters maintain the heat for the
well plates from underneath, to ensure a rapid evaporation
process and prevent the samples from cooling or freezing during
evaporation, see Figure 3.
Figure 3.
The evaporation process.
Gas Heaters
The gas heaters (see Figure 1) operate over a temperature range
from ambient to 65 °C. The heated gas is passed through the
manifold and nozzles into the well plates.
To determine the correct gas flow and temperature for
an evaporation run, see
"Determine Optimal Evaporation
.
Plate Heaters
The plate heaters (see Figure 1) operate over a temperature
range from ambient to 80 °C, heating the well plates from
underneath.
The exact plate height for an evaporation run can be specified
in the user interface. When an evaporation run is performed, the
lift (see Figure 1) will elevate the plate heater to the predefined
height.
If a cabinet door is opened, the evaporation run will pause
automatically. During the time period that the evaporation run
is paused, the gas flow and the heaters will stop. The plate
heaters will descend to the lowest position. When the door is
closed, the evaporation run will resume.
To determine the correct plate height for an evaporation run,
see "Determine Optimal Evaporation Conditions" on page 6.
Evaporation
To provide consistency and reliability, it is possible to apply
different settings to prevent an evaporation run from starting
before the gas heater and/or plate heater has reached the
correct temperature. Additionally, it is possible to ensure that
the gas and plate heater are kept at set temperatures before and
between runs. To adjust the settings for each heater and each
cabinet, see
"Evaporation Settings" on page 9.
Ventilation
Solvent vapors are removed using negative pressure applied
through the system exhaust fans and exit from the port at the
rear of the system (see Figure 2). While a laboratory fume hood
is an ideal location for installation of the system, any location is
acceptable if the system is adequately vented through a proper
ventilation system. For more information, see the
TurboVap
®
96
Dual Installation and Safety
.
Audible Alarm and Lighting
To provide better visibility for the user, two sets of LED lights are
located under the lid, one set for each cabinet. The brightness
can be adjusted independently for each cabinet, see "Common
Settings" on page 8.
Figure 4.
The TurboVap 96 Dual with the LED lights at different brightness
settings for the two cabinets.
When performing an evaporation run in Time mode or Method
mode, the system terminates the gas flow, changes the color of
the LED lights to green (see Figure 5), and sounds an alarm as
the specified evaporation time is reached. The alarm continues
to beep every 30 seconds until the Evaporation Finished dialog
is closed or the door is opened.
