BiOptic Qsep1 Скачать руководство пользователя страница 29

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5-1. Unpacking Cartridge: 

Please follow the steps of “Unpacking guide” inside the cartridge box.

 

5-2. Add 1X Separation Buffer into 

“S” well and add diH

2

O into others. 

* Buffer height should be equal to the groove of wells. 

 

 

5-3. Alignment Marker preparation: 

Add ≥ 20 μl “Alignment Marker 20 bp & 1000 bp” (C109100) into the 0.2 ml tube 

and then add 10 

μl “Mineral Oil” on the top of Alignment Marker.

 

6.

 

Click “Change Buffer”.

 

 

7.

 

Place Alignment Marker 20 bp & 1000 bp C109100 (≥ 20 μl) at MA-1 position 

and the buffer tray on the tray holder.

 

* Please make sure buffer tray have been placed correctly and fitted in the 

holder.

 

* Spin down and make sure the Alignment marker is in the bottom of tube and 

no air bubble appears. 

 

 

8.

 

Hold the plate by your hand and press down the tube of Alignment Marker 

tightly into the well by thumb.

 

Содержание Qsep1

Страница 1: ...BiOptic Inc 4F No 108 3 Minquan Rd Xindian Dist New Taipei City 23141 Taiwan R O C www bioptic com tw Revolution Qsep1 Qsep100 Operation Manual Hardware...

Страница 2: ...is prohibited without prior written permission of BiOptic Inc except as allowed under the copyright laws Qsep Series and Q Analyzer are registered trademarks of BiOptic Inc Microsoft Windows is regist...

Страница 3: ...0 1 1 3 Qsep100 Top View 11 1 2 Qsep1 Instrument 12 1 2 1 Qsep1 Front View 12 1 2 2 Qsep1 Back View 13 1 2 3 Qsep1 Top View 14 1 3 Hardware Requirements 15 2 Installation Instruction 16 2 1 Environmen...

Страница 4: ...r loss resulting from not complying or familiar with the manuals or improper operation of the devices Applications Genetic Profile Screening Amplified Fragment Length Polymorphisms Bacterial Viral Gen...

Страница 5: ...ce it upside down 4 Store the cartridge in the calm shell container Cartridge needs to be stored in a proper environment as the instructions of cartridge unpacking guide to maintain its lifetime and q...

Страница 6: ...ease disconnect the power plug 15 Please clean the instrument with dry cloth For other maintenance please contact local distributor 16 Please use the MAINS power cord within adequate rating 17 The con...

Страница 7: ...ument intuitively The disposable gel cartridge with integrated pre programmed test methods makes capillary electrophoresis experiments no longer a painful procedure that requires well trained operator...

Страница 8: ...when the high voltage source of the capillary electrophoresis is ON B Main Power switch The blue LED lights up when it is turned on C Operation room Open the sample door A rotation platform includes...

Страница 9: ...9 Figure 1 2 Rotation platform...

Страница 10: ...p100 back view A Air pressure gauge Display the value of the pressure operating in Qsep100 B Air flow Inlet Port Connect to an external pump using an 1 8 inches tube C USB Port Connect to a computer D...

Страница 11: ...11 1 1 3 Qsep100 Top View Figure 1 4 Qsep100 top view A Cartridge door...

Страница 12: ...12 1 2 Qsep1 Instrument 1 2 1 Qsep1 Front View A Indicator lamp B SD Card Port C Cartridge Door D Sample Door and Sample Tray...

Страница 13: ...13 1 2 2 Qsep1 Back View A Power Connector B Main Power switch C Air flow Inlet Port Connect to an external pump using an 1 8 inch tube D Cable Port...

Страница 14: ...14 1 2 3 Qsep1 Top View A Cartridge door A...

Страница 15: ...are Requirements Minimum Recommended CPU Intel Core i3 530 2933MHz Dual Core Intel Core i7 2 8 GHz RAM 4G RAM or More 8G RAM or More HDD 320GB or More 500GB or More PORT USB 2 0 x 1 USB 3 0 x 1 INTERN...

Страница 16: ...installation it is recommended to leave approximately 711 mm 28 inches clear space above the counter space or table Allow approximately 76 mm 3 inches of clear space at the rear and to the side of the...

Страница 17: ...source within the range of 50psi 75psi The input voltage of Qsep1 instrument is 12V DC and the maximum power is 80W A suitable power adapter with cord MEAN WELL GSM90B12 P1M is included in the package...

Страница 18: ...inside the portable air pump If this occurs be sure to release the condensation water before using Qsep Series If you need to continuously operate Qsep Series check the air pump every 12 hours to avoi...

Страница 19: ...r WiFi function to connect the instrument with your computer after Turn on Qsep1 Turn on the power wait 25 second for WiFi function initialization Green light flashing and install the operation softwa...

Страница 20: ...t the air tube 3 Plug the power cord labeled Qsep1 4 Switch on Qsep1 the LED lights multiple color light Fig 3 Wait 25 seconds for the instrument and Wi Fi initialization and the green LED will start...

Страница 21: ...sword The password will show in the sticker which is behind the instrument It is the same as the middle 8 digits of instrument serial number same as SSID name by blinding the prefixal Qsep1 Fig 7 If y...

Страница 22: ...the firewall 5 Check the LED status 6 Double click the Q Analyzer 7 Q Analyzer user interface 7 1 Select Network mode and click Search After Search the instrument ID will display Please confirm the in...

Страница 23: ...es Make sure no air bubble appears in each tube 9 Click Change Sample or Change Buffer the sample door will open automatically 10 Slide the buffer tray into the instrument Make sure sample tray is pus...

Страница 24: ...n New cartridge needs to be calibrated before using Please proceed with the following steps 14 1 Click HV Check The storage and transportation condition may influence the Gel matrix and cause unstable...

Страница 25: ...will only display after HV check passed For troubleshooting please refer to cartridge unpacking guide for details 15 To analyze samples click on the blank column and designate the sample locations tes...

Страница 26: ...lect a proper method for your application 15 3 Click on Result Name and enter the name of result files 15 4 Click the icon Para and set the parameters Baseline Factor Peak Threshold Calculate etc 16 C...

Страница 27: ...ease check the Alignment Marker has been located at correct position Software will recognize two peaks from Alignment Marker signal DO NOT use Size Marker or DNA sample doing Calibrate Brief introduct...

Страница 28: ...28 3 2 Qsep100 Quick Start 1 Turn on the power 2 Double click the Q Analyzer 3 Main window appears 4 Click Connect to link the instrument 5 Cartridge and Buffer preparation...

Страница 29: ...into the 0 2 ml tube and then add 10 l Mineral Oil on the top of Alignment Marker 6 Click Change Buffer 7 Place Alignment Marker 20 bp 1000 bp C109100 20 l at MA 1 position and the buffer tray on the...

Страница 30: ...the sample is in the bottom of tube and no air bubble appears 10 According to cartridge unpacking guide open the cartridge door and insert cartridge Guiding groove facing front 11 Close the cartridge...

Страница 31: ...ransportation condition may influence the gel matrix and cause unstable current Check current and see if it s stable or not After HV Check if current is still unstable please repeat this step 2 3 time...

Страница 32: ...plate and then press OK 13 2 Click Method to select analysis method If you want to analyze the samples with Alignment Marker please choose the appropriate Alignment Marker and then place it on the cor...

Страница 33: ...etc 14 Click Run to start the process During Calibration Please check the Alignment Marker has been located in correct position Software will recognize two peaks from Alignment Marker signal DO NOT u...

Страница 34: ...34 Introduction of the Control Panel...

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