biochrom HB Ultrospec 7500, Руководство пользователя, Страница: 62 / 104

Ultrospec 7500 User Manual • 41 56 2050 REV03 • 62
OR
Select the custom dye and define the dye absorbance max to between 300
and 950 nm, the dye extinction coefficient to between 10000 and
9999999, and the dye correction factor to between 0.001 and 0.999.
Step 8
Proceed to the next parameter screen using the right/forward arrow.
Step 9
Select the sample protein from one of the predefined protein names.
Each protein name has fixed associated parameters:
Protein Name Wavelength Molar Ext. Coeff. Ext. coefficient [L/(g*cm)]
BSA 280 nm 47790 0.689
SA (mouse) 280 nm 43780 0.637
SA (human) 280 nm 39310 0.567
IgG 280 nm 210000 1.400
Lysozyme 280 nm 37500 2.600
OR
Select the custom protein name, then set the protein quantification mode
to “Ext. Coefficient [L/(g*cm)]” or “Molar Ext. Coeff.” and define the
wavelength to between 200 and 340 nm, the molar extinction coefficient
to between 10000 and 9999999, and the mass extinction coefficient, or
molecular weight depending on the quantification mode, to between
0.001 and 9999999.
Step 10
Proceed to the next parameter screen using the right/forward arrow.
Step 11
Set display scan to “On” or “Off”.