biochrom HB Ultrospec 7500, Руководство пользователя

Страница: 61 / 104

Ultrospec 7500 User Manual • 41 56 2050 REV03 • 61
Step 4
Select the sample name to bring up the sample window, enter a sample
seed prefix and the incremental sample number starting value. Confirm
the settings using the confirm icon.
Step 5
Select one of the predefined units, “µg/ml”, “ng/µl”, “µg/µl”, or “mg/ml”.
Step 6
Proceed to the next parameter screen using the right/forward arrow.
Step 7
Select the dye type from one of the predefined dyes.
Each dye has fixed associated parameters:
Dye-Type Dye Abs Max Dye Ext Coeff Dye Correction
Alexa Fluor 350 346 nm 19000 0.190
Alexa Fluor 405 401 nm 34000 0.700
Alexa Fluor 488 495 nm 71000 0.110
Alexa Fluor 647 650 nm 239000 0.030
Cy3 550 nm 150000 0.080
Cy5 649 nm 250000 0.050
DyLight 649 654 nm 250000 0.040
DyLight 488 493 nm 70000 0.150
FITC 495 nm 68000 0.300
Pacific Blue 416 nm 46000 0.200
r-PE 566 nm 200000 0.180
Texas Red 595 nm 80000 0.180