BioCare Medical Decloaking Chamber Plus Скачать руководство пользователя

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Quick Instructions (continued)

After Run Completion

1. Confirm that the “Cycle Done!” message is displayed.

2. Visually confirm that the pressure has dropped to 0 (zero) psi.

3. Turn the unit off. Toggle the weight (petcock) to release any residual pressure.

4. Open the lid with the steam directed away from yourself.

5. Confirm that run has completed successfully by the following:

a. Steam Monitor Strip has changed to a dark brown or black color

b. Pressure gauge has reached between 17-24 psi.

6. Carefully remove the plastic slide containers from the instrument.

7. Place the plastic slide containers on the counter top to cool down for a at least 10 minutes.

8. Decant half of hot retrieval solution and add DI water. Do five complete changes of DI water.

9. Proceed with immunohistochemistry (IHC) staining .

Содержание Decloaking Chamber Plus

Страница 1: ...www biocare net U S patent number 6 580 056 START STOP DISPLAY SET P1 SET HH MM SS 00 00 30 125 0 Decloaking Chamber Plus Manual Digital programmable pressure system for heat induced epitope retrieval ...

Страница 2: ...Biocare Medical Technical Support 800 799 9499 Option 3 Customer Service 800 799 9499 Fax 925 603 8080 Corporate Office 60 Berry Drive Pacheco CA 94553 www biocare net ...

Страница 3: ...ded Use 1 Transportation Storage and Handling 2 Unpacking 2 Parts List 2 Description of Parts 3 Related Consumables 4 Important Notes 5 Important Safety Guidelines 6 Operating Instructions 6 Safety First 6 Assembly and Setup 6 Recommended Protocols 7 Beginning Operation 7 Programming 8 To Initiate a Run 8 After Run Completion 8 Positioning the Decloaking Chamber for Emergency Disconnection 11 Acti...

Страница 4: ...importance for consistent immunohistochemistry IHC staining The Decloaking Chamber is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety requirements Temperature pressure and pH can be monitored and recorded with the Decloaking Chamber to produce consistent staining which will reduce repeat testing and false negatives Specific...

Страница 5: ...ord any issues with regard to missing or damaged contents In the event the instrument is damaged in transit do not operate and contact Biocare Medical for instructions Operating Environment Laboratory conditions 15 30 C 15 80 relative humidity maximum elevation 2000 M Unpacking Review and follow the instructions on the Important Shipping Notice insert Review the Contents of Your Package Upon recei...

Страница 6: ...main body of the chamber that surrounds the heat sensor Heat Shield A circular concave metal disc with perforations on it that is placed in the center of the pan Rack Holder A circular stainless steel basket capable of holding plastic slide containers Cat No RMDC085 Weight Valve Regulates the amount of steam released Cat No RMDC038 Fuse Type 217 Fast acting 4 amp 250V Prevents electrical shorting ...

Страница 7: ... and CAP inspections Cat No 613 C D H QC Heat Tested pH Strips The pH of retrieval solutions at high temperatures can be critical for proper IHC staining However the pH of retrieval solutions can change at high temperatures To assure quality control and consistent staining Biocare Medical has developed heat tested strips that give accurate and true pH at high temperatures The pH of a solution at a...

Страница 8: ...n leading to malfunctions Avoid touching the pan or lid when in use HOT When in use the pan lid and weight get very hot avoid touching them There is a hot sticker to warn that the lid is hot The circular sticker reads HOT at elevated temperatures Do not operate the Decloaking Chamber near a heat flame or gas source Never place the instrument directly on a stove or other heat source Keep the exteri...

Страница 9: ...erators should wear gloves and eye protection when using the Decloaking Chamber Do not use alcohol or other flammable materials in the Decloaking Chamber Operating Instructions Safety First The Decloaking Chamber must be used only for its intended purpose following the operating instructions in this manual If the Decloaking Chamber is used in a manner not specified herein the safety protection pro...

Страница 10: ...ertinent clinical data by a qualified pathologist Recommended protocols for HIER with Decloaking Chamber Plus 125 C for 30 seconds 95 C for 40 minutes 90 C for 15 minutes 80 C for 60 minutes 60 C for 15 hours Beginning Operation Adjust the pan by matching the pan handles with the body handles Be certain that the outside of the pan is dry as any wetness will cause the Decloaking Chamber to make a c...

Страница 11: ...cord temperature and pressure Note that the USB flash drive if used will automatically record run except pressure data 8 When the alarm sounds a second time approximately 20 minutes the temperature has cooled to your programmed P2 fan off temperature After Run Completion 1 Confirm that the Cycle Done message is displayed 2 Visually confirm that the pressure has dropped to 0 zero psi 3 Turn the uni...

Страница 12: ...is not available place the heat shield in the bottom center of the pan 4a Place the container with the retrieval solution in the rack holder Do not use glass containers 4b If heat shield is used place the container with the retrieval solution on top of the heat shield Do not place the container on the center of the heat shield where the heat is concentrated but towards the edge of the heat shield ...

Страница 13: ...ip after being exposed to the temperature of 110 C or above for 30 seconds 6 Place the weight petcock onto the vent nozzle Making sure it is fully seated and rotates freely 7 Match the word OPEN on the lid to the white dot on the handle 8 Secure lid by turning clockwise until the word CLOSED is matched with the white dot on the handle 5 5a 5b 6 7 8 Open White Dot ...

Страница 14: ...to block access to the power switch the power cord or the receptacle into which the power cord is plugged Do not place other objects around the Decloaking Chamber that may impede access to the switch or power cord Actions In Case of a Malfunction In case of a malfunction disconnect the Decloaking Chamber from its power source The Decloaking Chamber may be disconnected from the power source by eith...

Страница 15: ...or antigen retrieval This feature can be disabled by setting the time to zero 0 To access the following displays press and hold the DISPLAY SET button for 3 seconds To cycle through the screens press the DISPLAY SET button Delayed Start Screen Displays the time the programmed run will be delayed before stating automatically Preheat Screen Displays the Preheat temperature setting and the duration o...

Страница 16: ...is temperature and relative pressure that a dark brown to black color is achieved Figure 5b Lower temperature and pressure will produce a lighter strip which will remain consistent for runs of the same temperature and pressure but it is easier to use the darker color that is achieved at higher temperature and pressure as a quality control Factory Settings Temperature Time Setting Delayed Start OFF...

Страница 17: ... is displayed 4 Press the DISPLAY SET button to change to minutes the MM will now begin blinking 5 Push the UP or DOWN arrow button until the correct minute is displayed 6 Press the DISPLAY SET button to change to seconds the SS will now begin blinking 7 Push the UP or DOWN arrow button until the correct second is displayed 8 Press the DISPLAY SET button to change from the Clock Set Screen to the ...

Страница 18: ...e counter reaches zero the program will move to the next step P2 Fan ON OFF until the end of the run when the alarm will sound for approximately 1 minute and the heat will turn off at this time it is recommended the User record the temperature and pressure for quality control The amount of time required to reach the programmed temperature and pressure are variable depending upon the voltage source...

Страница 19: ... Next Button Step 3 Change the Column data format of the first default column to DATE select YMD Step 4 Click the Finish button and the report will be displayed The report will show Column A Run Date Column B Run Time Column C Process Event Column D Run Temperature The Report Column C displays a code for the process events The key to this code is Event Description STA CYCLE START DSS DELAYED START...

Страница 20: ...that no water gets into the body Safety and Electrical Precautions Electrical Precautions Basic safety precautions should always be followed when using electrical equipment Do not immerse cord or plug or the body of the Decloaking Chamber in water or attempt to clean in a dishwasher Unplug the instrument when not is use and before cleaning Do not operate the instrument with a damaged cord or plug ...

Страница 21: ...urning the lid clockwise with the right hand until the lid etching that reads CLOSED is aligned with the white dot of the pan handle on the left side 8 The metal tabs on the lid should be tightly seated against the pan s lip Programming 1 Turn the instrument on by flipping the red toggle to the right of the control panel 2 Press and hold the Display Set button for 3 or more seconds to access the p...

Страница 22: ...rom yourself 5 Confirm that run has completed successfully by the following a Steam Monitor Strip has changed to a dark brown or black color b Pressure gauge has reached between 17 24 psi 6 Carefully remove the plastic slide containers from the instrument 7 Place the plastic slide containers on the counter top to cool down for a at least 10 minutes 8 Decant half of hot retrieval solution and add D...

Страница 23: ...ector Hot surface warning Power socket Symbol Glossary Troubleshooting and Service If for any reason the Decloaking Chamber fails to function correctly first double check the power connection then check the program for correct display of operating parameters There are no user serviceable parts only qualified trained personnel can service this equipment Please contact Biocare Medical Technical Supp...

Страница 24: ...800 799 9499 60 Berry Drive Pacheco CA 94553 www biocare net 904 003 071817 ...

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