13
Programming Display
(continued)
Audible Alerts
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Preheat:
3 beeps will sound when the set-point temperature is reached.
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End of Run:
The instrument will beep for 1 minute, indicating that the run is complete. To turn off the beeping tone at the end of
the run, press and hold the Start/Stop button for 3 seconds.
`
Display Buttons:
The display buttons beep each time they are pressed (this feature may be turned off for silent programming)
Default Settings
The User may program the run settings by entering the programming mode. With the power on, press and hold the DISPLAY/SET button
for 3 seconds. The User can now cycle through the screens and programmable parameters by pressing the DISPLAY/SET button once
for each parameter. Each parameter, when selected can be adjusted by pressing the UP or DOWN buttons until the desired setting
is displayed. When all the parameters have been set, press and hold the DISPLAY/SET button for 3 seconds to save the settings and
return to the main screen.
Below are the default settings recommended for optimal immunostaining. Preheat and Autocontinue are optional functions and not
necessarily required. Always, however, refer to the assay or analyte specification sheet for the specific antigen retrieval protocol as
times may vary.
The best temperature for Steam Monitor Strips to show quality control is 110 °C or above. It is at this temperature and relative pressure
that a dark brown to black color is achieved (Figure 5b). Lower temperature and pressure will produce a lighter strip, which will remain
consistent for runs of the same temperature and pressure, but it is easier to use the darker color that is achieved at higher temperature
and pressure as a quality control.
Factory Settings
Temperature
Time / Setting
Delayed Start
OFF
Preheat (optional function)
80 °C
30 seconds
Autocontinue (optional function)
ON
P1
125 °C
30 seconds
P2 Fan ON
100 °C
P2 Fan OFF
85 °C
Setting Range
Temperature
Time / Setting
Delayed Start
OFF – 23:59:59
Preheat
20 – 80 °C
80.5 – 95 °C
0 – 23:59:59
0 – 3:59:59
Autocontinue
ON / OFF
P1
80 – 99.5 °C
100 – 125 °C
0 – 1:59:59
0 – 0:29:59
P2 Fan ON
86 – 100 °C
P2 Fan OFF
37 – 95 °C
Changing the Default Settings
To change the factory settings press and hold the DISPLAY/SET button for 3 seconds. The first screen displayed is DELAYED START. If a
delayed start is desired press the DISPLAY/SET button once and the time HH (hours) will begin flashing. Use the UP or DOWN buttons
to adjust the hours. Press the DISPLAY/SET button again and the MM (minutes) will begin flashing. Adjust the minutes in the same
manner as the hours. Press the DISPLAY/SET button again and the SS (seconds) will begin flashing. Adjust the seconds in the same
manner as the minutes and hours. Press the DISPLAY/SET button again to move to the next screen. All other screens are programmed
the same way. When all screens have been programmed press and hold the DISPLAY/SET button to save the settings and return to
the main screen.
