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7

Control Panel Programming

1. If the Decloaking Chamber NxGen has already been run with the same protocol temperature and time as the current desired  
    run, press the circle button to go directly to either the USB or pre-run screen. Skip Steps 2-4 and go directly to Step 5.

2. If the current run will be different than the last run or the Decloaking Chamber NxGen is being used for the first time, press the 
    menu button to begin the protocol selection process.

3. Select the desired protocol temperature. 

a. There are 5 discrete temperature set points of 60ºC, 80ºC, 90ºC, 95ºC and 110ºC. 
b. Use the menu button (down arrow) to select the protocol temperature. The current selection will be highlighted.
c. Press the circle button to save the temperature selection.

4. Select the desired protocol time.

a. Each temperature set point is pre-programmed with a typical protocol time. After first use of a temperature, the time  
    for that protocol will default to the last used time. If using the optional 4-slot Metal Basket, use the recommended     
    time listed in the chart below as a starting point.
b. There are upper and lower maximums for each program time, please refer to the table below for details.

Program #

Temp

Default Time

Min Time

Max Time

Recommended time for 4 canisters

5

110ºC

15 minutes

1 second

30 minutes

18 minutes

4

95ºC

40 minutes

1 second

1.5 hours

42 minutes

3

90ºC

15 minutes

1 second

1 hour

25 minutes

2

80ºC

1 hour

1 second

2 hours

70 minutes

1

60ºC

12 hours

1 second

24 hours

12 hours

c. Use the clock button (up arrow) to select the program variable (hours, minutes or seconds) If you pass the correct 
   number, continue pushing the clock button and it will cycle back to 00. For faster cycling, hold down the clock button.
d. Use the menu button (right arrow) to move to the minute selection. It will become highlighted.
e. Use the clock button (up arrow) to select the next variable. 
f. Continue in this fashion until all variables have been programmed.
g. Press the circle button to save the temperature selection. There will be a pause of up to 5 seconds as the unit checks 
for a USB drive.

Note: Protocols for specific applications vary; these include, but are not limited to: fixation, heat-retrieval method, 
incubation times, tissue section thickness and detection kits used. Recommended incubation times and titers listed 
are not applicable to non-Biocare detection systems, as results may vary. Data sheet recommendations and protocols 
are based on use with Biocare products. It is the responsibility of the end user to determine optimal conditions; and 
use these tools in conjunction with other diagnostic tests and pertinent clinical data.

5. If a USB drive is not present, a USB reminder screen will appear. It will say “No Flash Drive”
    at the top of the screen.

a. To save run data, insert the USB drive supplied with the unit into the USB slot.
b. Press the circle button (check mark) to confirm the flash drive, there will be a pause 
    of up to 5 seconds as the software confirms the flash drive’s presence. 

i.  If the flash drive is okay, the unit will automatically advance to the pre-run screen.
ii. If the flash drive cannot be accessed, the unit will stay on the USB screen 
and show “No Flash Drive” at the top of the screen.
iii. Either adjust or replace the flash drive and press the circle button again.

c. To not save run data, do not insert a flash drive and press the clock button (X)  
    to continue to the pre-run screen. The Run Number will still be incremented.

Insert

USB

Содержание Decloaking Chamber NxGen

Страница 1: ...www biocare net U S patent number 6 580 056 Decloaking Chamber NxGen Manual Digital programmable pressure system for heat induced epitope retrieval ...

Страница 2: ...Biocare Medical Technical Support 800 799 9499 Option 3 Customer Service 800 799 9499 Fax 925 603 8080 Corporate Office 60 Berry Drive Pacheco CA 94553 www biocare net ...

Страница 3: ...Safeguards 4 Before First Use 5 Upon Receipt 5 Initial Set Up 5 Instructions for Use 6 Operating Instructions 6 Control Panel Programming 7 Program Completion 9 95 C Protocol Function 10 Reporting Software 11 USB Flash Drive 11 Software Reports 11 Software User Guide 11 Maintenance 13 Daily 13 Monthly 13 Troubleshooting Guide 14 Quick Instruction Guides 15 Instrument Operation 15 Software Reports ...

Страница 4: ... Decloaking Chamber NxGen is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety requirements Temperature pressure and time can be monitored and recorded with the Decloaking Chamber NxGen to produce consistent staining Specifications Intended Use The Decloaking Chamber NxGen is a programmable bench top temperature pressure cham...

Страница 5: ...ith a minimum capacity of 512 MB and a maximum capacity of 4 GB Waste Disposal All reagents used in the Decloaking Chamber NxGen must be properly disposed of in accordance with local state and federal regulations Instrument Disposal Dispose of the Decloaking Chamber NxGen in accordance with local state and federal regulations Decontaminate with an antibacterial cleansing agent Accuracy Temperature...

Страница 6: ... ON OFF Switch 11 Power Cord 12 Lid Handle 13 Lid 14 Sealing Ring Supporting Cover 15 Rubber Grommet 16 Upper Ring 17 Control Panel 18 Pressure Cable 19 Push Rod 20 USB Port 21 Home Screen a Menu Button b Circle Button c Clock Button 22 Metal Slide Canister 23 Slide Rack 24 Metal Rack Holder DC2012 1 3 4 6 7 8 a b 2 9 10 11 12 18 13 14 15 17 5 23 16 22 24 21 20 c 19 ...

Страница 7: ...aking Chamber NxGen will not be able to record the pressure during the run 7 Always check the pressure release device for clogging before use Do not operate without the pressure release properly installed 8 Do not place the Decloaking Chamber NxGen in a heated oven or on any heated surface Do not place the Decloaking Chamber NxGen near a flame or gas source 9 Keep the exterior of the unit dry To p...

Страница 8: ... to the ON position 6 Ensure the Decloaking Chamber NxGen is on the Home Screen a Current temperature of the unit b Software version c Consult manual before operating notice 7 Set the current date MM DD YYYY a Push the clock button on the home screen b Use the clock button up arrow to select the current month If you pass the current month continue pushing the clock button and the month will cycle ...

Страница 9: ... or filtered water and run them as if slides were placed into the canister d Place metal slide rack containing metal slide canisters into the metal water chamber Ensure the metal water chamber is positioned so that the white cup level markings face to the rear of the unit e For 110 C protocols place a dry Steam Monitor Strip on top of the metal slide canisters or metal slide rack 5 Before locking ...

Страница 10: ...g the clock button and it will cycle back to 00 For faster cycling hold down the clock button d Use the menu button right arrow to move to the minute selection It will become highlighted e Use the clock button up arrow to select the next variable f Continue in this fashion until all variables have been programmed g Press the circle button to save the temperature selection There will be a pause of ...

Страница 11: ...or is cooling down down arrow iii The selected protocol number protocol temperature and protocol time b In the middle of the screen the run screen displays iv A graphical representation of where the unit is at in the current run as represented by the circle on the graph iii i v ii vi vii iv c In the bottom of the screen the run screen displays the following v Unit current pressure in PSI vi It wil...

Страница 12: ... and the water replaced with room temperature deionized or filtered water to expediate the cool down 5 Use extreme caution when opening the lid Turn the lid clockwise and lift Tilt the lid at a 45º angle above the water pot and shake several times This will allow the water that has accumulated in the lid to drain Place the lid next to the unit a Use extreme caution when opening the lid Liquids and...

Страница 13: ...re has been achieved The following message will be displayed pictured below on the unit screen with two separate options for user input 1 If slides will be added to the hot retrieval solutions press the button under the Load slides option and open the unit to add the necessary slides Replace and secure lid The run will resume automatically with the user selected hold time after the unit temperatur...

Страница 14: ...e on the selected instrument If run data was collected from multiple Decloaking Chamber NxGen the Summary Report can be configured to list the run data from a single instrument or for all associated units at once Summary Report by Date The summary report by date generates a list of all of the runs performed between two user selected dates If run data was collected from multiple Decloaking Chamber ...

Страница 15: ...Click on the Summary Report by Instrument option 2 In the Serial option window select the serial number of the desired Decloaking Chamber NxGen from the drop down list Alternatively leave the Serial option set to All to generate a Summary List of all uploaded Decloaking Chamber NxGen run files 3 Use the drop down calendar in the Start Date and End Date option window to set the desired start and en...

Страница 16: ...in water or pour water into it 4 With warm water rinse the underside of the lid including the sealing ring pressure limit valve anti block cover air escape and pressure indicator poppit Dry completely 5 Clean area under upper ring with dampened cloth or microfiber cloth Do not use chemical cleaners 6 To clean sealing ring hold the knob on the sealing ring supporting cover and pull the sealing ring...

Страница 17: ...p lid to loosen the poppit and allow it to rise The metal water chamber may be dented or deformed Contact Biocare Technical Support Display is not showing anything Unit is not plugged in Plug the power cord into the unit and then into the wall outlet ON OFF switch is in the OFF 0 position Check to see if switch is in the ON position Message Appears ERROR No water in water chamber Put 500ml of deio...

Страница 18: ...mpletion and unit is under 95ºC carefully open the lid Tilt the lid at 45º and gently shake to drain excess water before fully removing the lid Use extreme caution as steam and liquids can still be very hot 10 Remove metal rack holder by grasping the plastic handles Do not grab the metal rack holder or the metal slide canisters by the metal it is very hot 11 Continue with slide processing 12 Once ...

Страница 19: ...ure sensitive steam strip allows the end user to monitor both pressure and heat inside the Decloaking Chamber NxGen The best temperature for a Steam Monitor strip to show quality control is 110ºC 5ºC It is at this temperature and relative pressure that a dark brown to black color is achieved Lower temperature and pressure will produce a lighter strip The steam strip can be dated and recorded for l...

Страница 20: ...ropean Community ETL Approved Manufacturer Serial number Hot Surface Consult instructions for use Caution consult accompanying documents Power USB connector Power socket Symbol Glossary Please contact Biocare Medical Technical Support if any problems arise 800 799 9499 Option 3 ...

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Страница 24: ...800 799 9499 60 Berry Drive Pacheco CA 94553 www biocare net 904 012 071817 ...

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