BIO RAD 750-0135, Инструкции для начального набора

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Step Start
Number (ml) Step
1. 0.0 Collect fractions of size 2.00 ml during entire run
2. 0.0 Turn chart recorder ON
3. 0.0 Isocratic flow with 100% 25 mM tris, pH 8.1,
0% 25 mM tris, 0.5 M NaCl, pH 8.1 at 4.00 ml/min
for 1.0 ml
4. 1.0 Set UV baseline to 0.0
5. 1.0 Static loop: Inject 0.5 ml sample at 4.00 ml/min for
0.1 min
6. 1.5 Isocratic flow with 100% 25 mM tris, pH 8.1,
0% 25 mM tris, 0.5 M NaCl, pH 8.1 at 4.00 ml/min
for 0.8 ml
7. 2.3 Linear gradient with 0% to 50% 25 mM tris,
0.5 M NaCl, pH 8.1 at 4.00 ml/min for 13.0 ml
8. 15.3 Isocratic flow with 0% 25 mM tris, pH 8.1,
100% 25 mM tris, 0.5 M NaCl, pH 8.1 at
4.00 ml/min for 2.8 ml
9. 18.1 Isocratic flow with 100% 25 mM tris, pH 8.1,
0% 25 mM tris, 0.5 M NaCl, pH 8.1 at 4.00 ml/min
for 8.0 ml
10. 26.1 End of protocol
3. When you have finished programming the Protocol Editor, click on the
toolbar button RUN . You will see a dialog box asking you to name the
run. For now, type in Run 1 and click the OK button. You will now see
the Run Control Screen (see Figure 13).
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