Berthold 55393 BA2, Руководство по эксплуатации

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Quick Start LB 983 NightSHADE II Operating Manual
38
Select to define the measurement workflow.
Four different kinds of meaurements can be performed. Please note that depending on the sys-
tem configuration some of the selections might not be possible (e.g. due to a missing fluores-
cence module).
To insert a luminescence measurement into the workflow select
“ Luminescence…”
. In the up-
coming parameter settings you can define the exposure parameters like the exposure time, the
camera gain, the readout speed of the camera and the pixel binning rate.

Exposure Time
is the time when the CCD chip is exposed to the sample

Gain
is an internal conversion factor. Low Gain offers high dynamic range while high gain
gives higher intensity numbers but limited dynamic range.

Read out
defines the speed to read out the camera. High speed is recommended for
phgotographioc images, high intensity measurements of fluorescence images since a
higher background noise will be obtained. Low speed is ideal for highest sensitivity lu-
minescence measurements but trhe camera readout can take up to 15 seconds.

Binning Factor
is the combination of neighbouring CCD pixels during readout. The result is an in-
creased signal and thus an improved sensitivity and a better signal-to-noise ratio, how-
ever, at the cost of loss in spatial resolution.