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Vi-Cell XR Cell Viability Analyzer

User Instructions

Written by CMCresson 15-Feb-06

The Vi-Cell XR Cell Viability Analyzer is a video imaging system used to analyze yeast, insect
and mammalian cells.  It automates the trypan blue exclusion protocol, in which dead cells take
up the dye while live cells do not, and provides data on percent viability and cell counts.
Essentially, the Vi-Cell system is an automated hemocytometer unlike the multisizer, which was
simply a particle counter.  The Vi-Cell takes up the sample and delivers it to a flow cell and
camera for imaging where differences in the grey scale between live and dead cells is determined
by the software.  For every sample you run, the instrument requires 500 

µ

L of sample volume,

which is mixed 1:1 with trypan blue, and takes 50 images to determine the cell concentration and
viability.  All of this data is output on the computer screen and can also be sent to a printer or
excel spreadsheet for further analysis.

System Specs

Cell Diameter Range = 3 to 70 

µ

m

Cell Concentration Range = 5 x 10

4

 to 1 x 10

7

 cells/mL

Viability Range = 0% - 100%
Counting Accuracy = 

±

6%

Sample Volume = 0.5 mL to 2.0 mL
Acquisition Time = 2 minutes

Getting Started with the Vi-Cell

Everyone 

who will be using the Vi-Cell system

 must 

be trained by the system administrator.  In

addition, 

everyone will be given a user account and password to log on to the instrument.

There will be a main system log in which user activity will be recorded.  So, if you allow
someone else to use your account, then you will be the responsibility party in the use log if
something happens to the instrument.   UROPs will not be given user accounts and will be the
responsibility of their lab advisor.

The Vi-Cell system default cell type may work for some cell lines in the lab and it is really the
best place to start.  However, since trypan blue uptake, cell size, and appearance are unique to
each cell type you should run some tests to determine the best parameters with your particular
cell line. For cell lines that are used by many lab members and that need changes to the default
parameters, we will create a cell type that will be specific for those cells.  The cell types will
appear in every user’s menu.  The software contains a tutorial for creating your cell lines so this
may be a helpful feature for more difficult cell lines. In addition, you can email cell images to
Beckman Coulter and they can assist you in determining the best parameters for your particular
cell line.

It is also important to be aware of the amount of time your samples will be sitting at room
temperature waiting on the carousel.  Each sample takes 2 minutes to run and the carousel holds
12 samples.  You may not want to prepare more than 12 samples to run at any give time as the

Содержание Vi-Cell XR

Страница 1: ...tem administrator In addition everyone will be given a user account and password to log on to the instrument There will be a main system log in which user activity will be recorded So if you allow someone else to use your account then you will be the responsibility party in the use log if something happens to the instrument UROPs will not be given user accounts and will be the responsibility of th...

Страница 2: ...isplayed in the main window Camera Image allows for the viewing of sample images during and after a run Autosampler Queue shows the completed samples along with the list of samples in the queue this is where you can modify remove samples that are already in the queue Cell Types displays windows with the information for each specific cell type this is where you create or remove new cell types Biopr...

Страница 3: ...ue to enter samples so it saves time to start the queue first 5 From the navigation menu choose Autosampler queue to see your samples in the queue From this screen you can also edit remove samples in the queue while a run is in progress 6 Click on Start queue to begin sample analysis Once the run begins your sample will disappear from the queue and you will only see it on the main screen The botto...

Страница 4: ...roll down to click on reanalyze At this point the default cell type window will open up and you can adjust the necessary parameters and click ok The cell images will then be analyzed using the new parameter values and the data will be displayed on the right side of the screen Reanalyzing a sample does not overwrite the original data and the new data is not automatically saved So you need to choose...

Страница 5: ...spending your cells in a solution that is also hazardous you need to write that information on the waste card as well every time you use the cell counter As for the instrument reagents these will be ordered from Beckman Coulter by the lab manager and stocked near the instrument Only the Beckman Coulter reagents are compatible with this instrument Do NOT use any other brand of trypan blue If we are...

Страница 6: ...ed on spot brightness spot area Live Cell Reject Non Circular Objects Separate Cells from Background Based on brightness sharpness Vi CellXR Cell Image Analysis Scheme Not Viable Debris Meets Min Circularity Viable Decluster Cells Dead Cell ...

Страница 7: ...delete a cell type 2 choose File from toolbar then click on Image Reanalyze and you will be given the option to add modify or delete a cell type 3 choose Instrument Reanalyze from toolbar then you will be given the option to add modify or delete a cell type Sample Prep Options Parameter Range Default Details Aspiration Cycles 1 to 7 3 of times sample is mixed in syringe set according to cell sensi...

Страница 8: ...o include more objects Increase to include less objects Spot Brightness 75 Determines cell viability Increase value more cells counted as dead Decrease value more cells counted as live Spot Area 1 20 5 Ratio of the bright spot to total area Determines cell viability Increase value more cells counted as dead Decrease value more cells counted as live Minimum Circularity 0 0 5 0 Excludes dead cells s...

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