Beckman Coulter Vi-Cell XR Скачать руководство пользователя страница 8

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CMCresson

15-March-06

Parameter

Range

Default

Details

Images

1 – 100

Increase value for very low concentration samples

Size

3 - 70

10 -40

If a particular cell is being problematic, place
cursor over cell and size is displayed below

image

Brightness

75 – 90

Picks out objects from background
Increase includes more objects
Decrease includes less objects

Sharpness

60 – 200

100

Picks out objects from background
Decrease to include more objects
Increase to include less objects

Spot

Brightness

75 %

Determines cell viability
Increase value more cells counted as dead
Decrease value more cells counted as live

Spot Area

1 – 20 %

5 %

Ratio of the bright spot to total area
Determines cell viability
Increase value more cells counted as dead

Decrease value more cells counted as live

Minimum

Circularity

0–0.5

Excludes dead cells so they are considered debris
0 excludes no dead cells
1 excludes all dead cells

Decluster

None, low,

med, high

medium

Ability of software to pick individual cells from a
cluster of multiple cells
None counts a cluster as 1 cell

Содержание Vi-Cell XR

Страница 1: ...tem administrator In addition everyone will be given a user account and password to log on to the instrument There will be a main system log in which user activity will be recorded So if you allow someone else to use your account then you will be the responsibility party in the use log if something happens to the instrument UROPs will not be given user accounts and will be the responsibility of th...

Страница 2: ...isplayed in the main window Camera Image allows for the viewing of sample images during and after a run Autosampler Queue shows the completed samples along with the list of samples in the queue this is where you can modify remove samples that are already in the queue Cell Types displays windows with the information for each specific cell type this is where you create or remove new cell types Biopr...

Страница 3: ...ue to enter samples so it saves time to start the queue first 5 From the navigation menu choose Autosampler queue to see your samples in the queue From this screen you can also edit remove samples in the queue while a run is in progress 6 Click on Start queue to begin sample analysis Once the run begins your sample will disappear from the queue and you will only see it on the main screen The botto...

Страница 4: ...roll down to click on reanalyze At this point the default cell type window will open up and you can adjust the necessary parameters and click ok The cell images will then be analyzed using the new parameter values and the data will be displayed on the right side of the screen Reanalyzing a sample does not overwrite the original data and the new data is not automatically saved So you need to choose...

Страница 5: ...spending your cells in a solution that is also hazardous you need to write that information on the waste card as well every time you use the cell counter As for the instrument reagents these will be ordered from Beckman Coulter by the lab manager and stocked near the instrument Only the Beckman Coulter reagents are compatible with this instrument Do NOT use any other brand of trypan blue If we are...

Страница 6: ...ed on spot brightness spot area Live Cell Reject Non Circular Objects Separate Cells from Background Based on brightness sharpness Vi CellXR Cell Image Analysis Scheme Not Viable Debris Meets Min Circularity Viable Decluster Cells Dead Cell ...

Страница 7: ...delete a cell type 2 choose File from toolbar then click on Image Reanalyze and you will be given the option to add modify or delete a cell type 3 choose Instrument Reanalyze from toolbar then you will be given the option to add modify or delete a cell type Sample Prep Options Parameter Range Default Details Aspiration Cycles 1 to 7 3 of times sample is mixed in syringe set according to cell sensi...

Страница 8: ...o include more objects Increase to include less objects Spot Brightness 75 Determines cell viability Increase value more cells counted as dead Decrease value more cells counted as live Spot Area 1 20 5 Ratio of the bright spot to total area Determines cell viability Increase value more cells counted as dead Decrease value more cells counted as live Minimum Circularity 0 0 5 0 Excludes dead cells s...

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