CMCresson
15-March-06
Creating and Modifying Vi-Cell Cell Types
When you begin using the instrument, it is best to select the
Default Cell Type
to analyze your sample. Then, you can adjust these parameters to fit the
specific cell line you are measuring and create a unique cell type. These cell
types will show up in everyone’s user account and there should be only
one cell
type for each cell line
that we use in the lab. Please talk with other lab
members to set the parameters for a given cell line so that everyone can use
the same cell type for a given cell line.
For additional details, please see the Vi-Cell User Manual or the cell type power
point tutorial, which is saved on the desktop of the Vi-Cell computer with the
extension pps.
To access the cell types:
1.
choose
File
from toolbar then click on
Cell Type
and you will be given
the option to add, modify, or delete a cell type.
2.
choose
File
from toolbar then click on
Image-Reanalyze
and you will be
given the option to add, modify, or delete a cell type.
3.
choose
Instrument-Reanalyze
from toolbar then you will be given the
option to add, modify, or delete a cell type.
Sample Prep Options
Parameter
Range
Default
Details
Aspiration
Cycles
1 to 7
3
# of times sample is mixed in
syringe; set according to cell
sensitivity & clumpiness
Trypan Blue
Mixing
1 to 7
3
# of times sample and trypan blue
are mixed in syringe
Image Analysis Parameters
The ranges given below are the normal recommended ranges for each
parameter and default value refers to the parameter value in the
Default Cell
Type
. The default values are a good starting place and you should tweak
individual parameters to determine their effects on the output values.
For the cell brightness parameter, you will need to observe the image in binary
mode to see the difference between parameter values. Right click on the
image, then
turn off annotation
and then choose
Binary Mode.
Return to
normal view for all other parameters.
