Beckman Coulter COULTER LH 750 System, Справочник

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PN 4277248DD
3-5
OPERATION PRINCIPLES
AUTOMATIC ASPIRATION MODE
3
After aspiration of the blood sample, the sliding plate of the diff segment valve (shear valve)
moves, aligning the reagent input and output ports with the diff segment isolated in the
stationary plate of the valve.
The diff lytic reagent pump dispenses diff lytic reagent, displacing the reagent in the diff
heater. The reagent from the diff heater flows through the diff segment valve, diluting the diff
blood segment into the mixing chamber.
In the mixing chamber, the blood and diff lytic reagent mixture is incubated and mixed.
Then the diff preservative pump dispenses diff preservative into the mixing chamber. The
final dilution of blood, diff lytic reagent and diff preservative is mixed and incubated.
Diff sample pressure is applied to the mixing chamber to push the final incubated dilution of
sample from the mixing chamber into the sheath fluid stream in the flow cell for sensing.
Before the next sample is analyzed, the diff sample segment, the mixing chamber, and the
flow cell are rinsed with diluent, and the diff sample segment and diff sample delivery line are
primed with diff lytic reagent.
The diff lytic reagent pathways to and from the diff sample valve remain primed with diff lytic
reagent.
Reticulocyte (Retic)
During aspiration of the blood sample, blood is pulled through two shear valves before it
reaches the rear blood detector.
After aspiration of the blood sample, the sliding plate of the retic segment value (shear valve)
moves, aligning the reagent input and output ports with the retic segment isolated in the
stationary plate of the valve.
The stain pump dispenses retic stain through the retic segment valve, washing the retic
segment into the stain chamber. Depending on the stain chamber temperature, the stain
chamber heater may be activated to enhance the staining reaction.
After incubation, the 50 µL blood /stain aspiration pump is activated twice to pull a stained
sample from the stain chamber and through the stained retic segment valve.
The retic clearing solution pump dispenses 2 mL of retic clearing solution, displacing the
reagent in the Peltier module. The reagent from the Peltier module flows through the sample
segment in the stained retic segment valve, diluting approximately 2 µL of the stained blood
sample into the retic chamber.
In the retic chamber, the stained blood and retic clearing solution mixture is mixed and
incubated.
Retic sample pressure is applied to the retic chamber to push the mixture from the retic
chamber into the sheath fluid stream in the flow cell for sensing.
Before the next sample is analyzed the retic segment tubing, retic chamber and flow cell are
rinsed with diluent, and the retic segment tubing and the retic sample delivery line are
primed with stain.