2-12
Learning to Create Methods for the Biomek® 3000
Beckman Coulter, Inc.
2.4.4
Configuring Destination Labware
To configure the labware named
Dest1
as destination labware in the
Transfer
step:
1. Before you configure the destination labware, click the down arrow in
Transfer
Details
located just above the Current Deck Display to collapse that section. You
will not be making any changes to the options in this section.
2. Click on
Dest1
sitting on
P6
in the Current Deck Display. This one operation
accomplishes the same task as steps 1 and 2 of Section 1.5.2
Note:
Notice that the
source
labware configuration is now replaced with a brief
sentence summary of the setup. If you want to reopen this source configuration
for any reason, click anywhere in the collapsed configuration area.
3. The
Volume
field is highlighted, which allows you to designate the amount of
liquid to be dispensed. For this tutorial, you’re transferring 100 µL; so, type
100
into the
Volume
field.
Note:
The volume is measured in microliters (µL) automatically and indicates
the amount of liquid that will be dispensed (100 µL) into each of the 96 wells; so,
in this case, you’re dispensing a total of 9600 µL into the 96-well microplate.
4. In
of
, select
Water_MP200_P200
as the liquid type, if necessary. Biomek
Software comes with three liquid type definitions for water:
Water
,
Water_MP20_P20
, and
Water_MP200_P200
. You should always use the
definition that includes the tool used for the transfer for best results.
5. Right-click on the large tip illustration and choose
Measure from Bottom
.
6. Set the dispense height in the large tip illustration to
4.00 mm
from bottom
,
using the same technique as you used for setting the aspirate height.
Note:
If you accidentally open too many destination configurations, all you
have to do is right-click on the title above the graphic of the plate in the
configuration. Click on
Delete
from the popup menu and the entire
configuration goes away.
Transfer details include a
stop condition for
transfer operations; a
repeat pipetting
configuration; and a
maximum tolerance in
timing between transfer
operations. Refer to the
Biomek Software User’s
Manual,
.
Transfer Details
