Beckman Coulter Airfuge, Инструкция по эксплуатации

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2-6
Preinstallation Requirements
TRIAL RUN
When installation is complete, perform a trial run to confirm proper
ultracentrifuge operation. Refer to Section 3 for operating instruc-
tions. Time the rotor deceleration as follows.
1. After the rotor runs for a few minutes, turn the TIME dial to zero
and begin timing. The run light should go out approximately
2 minutes after the TIME dial is turned off.
2. Observe rotor motion through the window. Fixed angle rotors
should stop within about 2 minutes after the run light goes out.
Other rotors should stop within about 3-
1 /
2
minutes.
➠
NOTE
If a fixed angle rotor stops in less than
45 seconds, sample stirring may result; adjust
the brake or levitation air to provide a longer
stopping time. If a rotor takes too long to stop,
the brake, pin tension, or levitation air needs
adjusting. See Section 5 for adjustment
procedures.
COLD ROOM INSTALLATION
If rotor temperatures below room temperature are required, the
Airfuge can be operated in a cold room. A typical cold room set-up is
shown in Figure 2-3. In addition to the installation procedures above,
the following variations are required.
• Because large amounts of water may condense in the airstream
as it passes through the lower temperatures of the cold room, a
second filter is required to remove the additional water. Install
the second filter as close as possible to the ultracentrifuge. The
maximum distance between the filter and the instrument should
be 0.6 m (2 ft).
• The second filter will cause a pressure drop of about 5 psig
(35 kPa) in the air pressure that reaches the instrument. Make
sure that the required operating air pressure is obtainable at the
instrument pressure gauge.