BAS SEC2000 Spectra System, Руководство по эксплуатации

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SEC2000 Operating Manual
SEC2000 Spectra System
www.als-japan.com
54
⑥
On the step of ” Quantitative Analysis- Calibration ”, to measure the absorbance of
the standard material with known concentration, put a standard sample into the
cuvette holder, and input data in “ Concent. ” Column. Move your mouse to
“ Absorb. ” column, and click “ Get Absorb. ” icon to calculate the area of the
standard sample (get a calibration line with at least three different concentrations of
standard material).
<Fig. 6-2-6> Absorbance of K
3
Fe(CN)
6
solutions (Concentration: 1, 0.5 and 0.25 mM).
⑦
Click “ Use ”, then calibration line is obtained as shown in Fig. 6-2-7.
<Fig. 6-2-7> Calibration line with three different concentrations.
The standard samples are shown in closed circle.