17
Concentration Mode
1. Fill a cuvette with the solvent that will be
used to zero the spectrophotometer. This is
the blank (or background) solvent that your
sample is dissolved in.
2. Place the cuvette in the cuvette holder in the
sample compartment. Close the sample
compartment lid.
3. Press (
λ
nm) button, enter the numerical
value (330-999) of the wavelength and press
the ENTER button. If a mistake is made in
the key-in process, press the ESC button to
clear the display for a new entry.
4. Select Transmittance or Absorbance mode
by pressing the (T) or (A) button.
5. Zero the instrument using the blank solution
by pressing the (100%T/OA) button. The
display should show:
XXX
TCALB
or
XXX
ACALB
then should read
XXX
100.0T
or
XXX
0.000A
(Transmittance mode)
(Absorbance mode)
6. Carefully open the sample compartment and
remove the cuvette.
7. Fill a cuvette with a Standard solution of
known concentration and place the cuvette
in the sample compartment.
O
PERATION
