15
O
PERATION
Note
It is suggested to run the
Wavelength Check Mode to
ensure the 000.0 nm position
after a large number of
samples have been run or
after stand-by mode.
Operation procedures
Turn the power on, and allow the instrument
to warm up for at least 20 minutes.
After an internal self-test for initializaiton
calibration of the instrument is performed,
the system will proceed to first memory
mode automatically. The first memory mode
is pre-set for wavelength at 440 nm and for
Factor at 1 when the instrument is delivered.
The display should show:
Percent Transmittance and
Absorbance mode
1. Fill a cuvette with the solvent that will be
used to zero the spectrophotometer. This is
the blank (or background) solvent that your
sample is dissolved in.
2. Place the cuvette in the cuvette holder in the
sample compartment. Close the sample
compartment lid.
3. Press (
λ
nm) button, enter the numerical
value (330-999) of the wavelength and press
the ENTER button. If a mistake is made in
the key-in process, press the ESC button to
clear the display for a new entry.
4. Select Transmittance or Absorbance mode
by pressing the (T) or (A) button.
440 T
W
AITING
BLANK
