20
The HEPA filter consists of a continuous sheet of glass fibers pleated over rigid corrugated separators and
mounted in a rigid frame. It is very delicate and the filter media should
never
be touched.
Proven efficiency in all HEPA filters used in Baker cabinets is 99.99% for particles 0.3 microns in diameter.
The 0.3 micron particle is used as the basis for filter definition because theoretical studies have shown that
filtration efficiency should be at a minimum for particles of this diameter, with efficiency increasing for
particles either larger or smaller. Experiments with various viruses and microbial agents have proven the
effectiveness of HEPA filters. (Contact The Baker Company for more information)
It must be pointed out that the HEPA filter does
not
filter gasses or vapors, nor are they 100% efficient on
particulates. Consider the filters’ inefficiency when performing a risk assessment. Since this cabinet is
partially recirculating, there will be gaseous buildup to the point of equilibrium if these agents are used.
The life of a filter is determined by the cabinet motor/blower system design and how it is used and how
often. Under normal laboratory conditions, you can expect at least five years of use. However, misuse or a
heavy dust load within the cabinet will shorten any filter's lifetime. Bunsen burners and misuse of chemicals
will also shorten the useful life.
Reminders
After many years of seeing our equipment used and misused, we have made up this list of suggestions:
Store equipment and supplies outside of the cabinet.
Always leave the blower on.
Set the view screen at the proper height.
When possible, use pipetting aids.
Avoid use of an open flame within the cabinet unless the use has been specifically approved by a
safety professional.
Always keep the air intake grills clear and unobstructed.
Only use toxic, explosive or flammable substances if a safety professional has approved them for
work in your cabinet.
The control system will adjust automatically for small changes in the load on the blower.
Adjustments for larger changes should only be made by a qualified technician. Don't change the
cabinet or blower speed unless the change is required by a decrease in measured air velocity.
Work only with agents assigned to biosafety Levels 1, 2, or 3 in this cabinet.
Always close the drain valve after each use.
If the unit presents a warning signal, don't operate the unit until the warning ceases.
If the operators are well trained and use good common sense when operating your cabinet, you should have
very few problems.
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