Amersham Biosciences Hoefer DALT, Руководство пользователя, Страница: 56 / 70

Gradient Gel Solutions Hoefer DALT System
52
Amersham Biosciences
For 23 gels, 1.5 mm
900 ml each Heavy and Light, by volume in ml:
Light Solution
Heavy Solution
Final %T 7 8 9 10 11 12 13 14 15 16 17 18 19
Acryl. Stock
210 240 270 300 330 360 390 420 450 480 510 540 570
1.5M Tris-Cl,
pH 8.8
225 225 225 225 225 225 225 225 225 225 225 225 225
Water
445 415 385 355 326 296 266 236 206 176 146 116 86
10% SDS
9 9 9 9 9 9 9 9 9 9 9 9 9
Glycerol
0 0 0 0 0 0 0 0 0 0 0 0 0
10% APS
9 9 9 9 9 9 9 9 9 9 9 9 9
10% TEMED
2.21 1.93 1.71 1.54 1.40 1.29 1.19 1.10 1.03 0.96 0.91 0.86 0.81
Final %T 8 9 10 11 12 13 14 15 16 17 18 19 20
Acryl. Stock
240 270 300 330 360 390 420 450 480 510 540 570 600
1.5M Tris-Cl,
pH 8.8
225 225 225 225 225 225 225 225 225 225 225 225 225
Water
360 330 300 270 240 210 180 150 120 90 60 30 0
10% SDS
9 9 9 9 9 9 9 9 9 9 9 9 9
Glycerol
61 61 61 61 61 61 61 61 61 61 61 61 61
10% APS
4.50 4.50 4.50 4.50 4.50 4.50 4.50 4.50 4.50 4.50 4.50 4.50 4.50
10% TEMED
0.64 0.58 0.51 0.47 0.42 0.40 0.37 0.34 0.32 0.31 00.29 0.27 0.25