Amersham Biosciences Hoefer DALT, Руководство пользователя, Страница: 52 / 70

Homogeneous Gel Solutions Hoefer DALT System
48
Amersham Biosciences
23 gels, 1.5 mm
1800 ml. Amounts shown in ml.
12 gels, 1.5 mm
1300 ml.
Amounts shown in ml.
Final %T
7 8 9 10 11 12 13 14 15 16 17 18 19
Acrylamide
Stock 420 480 540 600 660 720 780 840 900 960 1020 1080 1140
1.5M Tris-
Cl,
pH 8.8 450 450 450 450 450 450 450 450 450 450 450 450 450
Water 890 830 771 711 651 591 532 472 412 352 292 232 172
10% SDS 18 18 18 18 18 18 18 18 18 18 18 18 18
10% APS 18.00 18.00 18.00 18.00 18.00 18.00 18.00 18.00 18.00 18.00 18.00 18.00 18.00
10% TEMED 4.41 3.85 3.42 3.08 2.79 2.57 2.38 2.20 2.05 1.93 1.82 1.71 1.62
Final %T
7 8 9 10 11 12 13 14 15 16 17 18 19
Acrylamide
Stock 257 293 330 367 403 440 447 513 550 587 623 660 697
1.5M Tris-
Cl,
pH 8.8 275 275 275 275 275 275 275 275 275 275 275 275 275
Water 544 507 471 434 398 361 325 288 252 215 179 142 105
10% SDS 11 11 11 11 11 11 11 11 11 11 11 11 11
10% APS 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00
10% TEMED 2.70 2.35 2.09 1.88 1.71 1.557 1.45 1.34 1.25 1.18 1.11 1.05 0.99