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Step 1. Set up and heat the oven
Hybridization Oven Calibration Procedure
3
Step 1. Set up and heat the oven
1
Install the
hybridization oven rotator rack
into the
hybridization oven
.
2
Load the
hybridization oven rotator rack
with the typical number of
hybridization chambers
used during a single hybridization run.
Install the
hybridization chambers
without microarrays. Distribute the
hybridization chambers
across
hybridization oven rotator rack
positions
to mimic normal operating procedure.
3
Turn on the
hybridization oven
, set rotational speed control to 20 rpm,
and set oven temperature consistent with the recommended
hybridization temperature for the protocol that you use.
4
Allow the
hybridization oven
to heat and stabilize for at least
3
hours.