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Agilent Technologies G2545A, Руководство по калибровке

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Hybridization Oven Calibration Procedure

Required Parts and Tools

Part

Vendor and part/model

hybridization oven

Agilent p/n G2545A

hybridization oven rotator rack

Agilent p/n G2530-60029

hybridization chambers

Agilent p/n G2534A 

Fourtec MicroLite II USB digital recording 
thermometer or equivalent

•

Range -40°C to 80°C

•

Resolution 0.1°C

•

Accuracy ±0.3°C

MicroDAQ.com

•

p/n LITE5008P (8,000 readings)

•

p/n LITE5032P (32,000 readings)

Computer and software to work with the 

digital 

recording thermometer

Fourtec DataSuite Software or other software 
as specified by the 

digital recording 

thermometer

 vendor

Calibration service to 65°C for 

digital recording 

thermometer

MicroDAQ.com p/n CAL-NIST-TEMP or other 
service as recommended by 

digital recording 

thermometer

 vendor

general-purpose labeling tape 

or

2 cable ties (long enough to attach the digital 
recording thermometer to the oven rotator rack)

Содержание G2545A

Страница 1: ...2545A Hybridization Oven Installation Operation and Maintenance Guide p n G2545 9001 for information on site requirements and oven placement Calibrate the Agilent G2545A Hybridization Oven After first installation in a working environment After each 3 months of use When systematic high microarray background noise may indicate a drift in oven temperature Do the calibration steps in this guide to ma...

Страница 2: ...cy 0 3 C MicroDAQ com p n LITE5008P 8 000 readings p n LITE5032P 32 000 readings Computer and software to work with the digital recording thermometer Fourtec DataSuite Software or other software as specified by the digital recording thermometer vendor Calibration service to 65 C for digital recording thermometer MicroDAQ com p n CAL NIST TEMP or other service as recommended by digital recording th...

Страница 3: ...used during a single hybridization run Install the hybridization chambers without microarrays Distribute the hybridization chambers across hybridization oven rotator rack positions to mimic normal operating procedure 3 Turn on the hybridization oven set rotational speed control to 20 rpm and set oven temperature consistent with the recommended hybridization temperature for the protocol that you us...

Страница 4: ...rt the Fourtec DataSuite Software The program opens in Map View 3 Remove the cover on the Fourtec MicroLite II USB digital recording thermometer and insert the digital recording thermometer into a USB port on the computer 4 Check that the Fourtec MicroLite II USB digital recording thermometer is detected by the computer When the Fourtec MicroLite II USB digital recording thermometer is connected t...

Страница 5: ...t the recorder 8 Set Interval to 00 05 00 5 minutes 9 Click Setup 10 Click Yes to continue 11 Remove the unit from the USB port and replace the cover 12 Put the digital recording thermometer onto the middle of the hybridization oven rotator rack 13 Secure the digital recording thermometer to the hybridization oven rotator rack Use general purpose laboratory labeling tape or cable ties ...

Страница 6: ...er from the oven Let the oven continue to operate after you remove the digital recording thermometer from the oven CAUTION Make sure that the digital recording thermometer is secure and will not detach from the rack If the digital recording thermometer comes loose during use the data collected can be inaccurate A loose digital recording thermometer that becomes trapped and prevents the rotator rac...

Страница 7: ... USB port on the computer 3 Right click the Logger icon and click Stop to stop data recording 4 Right click the Logger icon and click Download Data The Fourtec DataSuite Software automatically saves the data on the computer 5 Double click the Logger icon to view a graph of the data 6 To view archived data a Click File Open b In the Open Data Files dialog box select the time period and logger for w...

Страница 8: ...al recording thermometer 8 Hybridization Oven Calibration Procedure Figure 1 Temperature reading Period of stable temperature is highlighted Take the temperature reading from the period of stable temperature data recorded before the oven door is opened ...

Страница 9: ...he difference between the two temperature readings is more than 0 2 C a Press both and at the same time until the two outside decimal points of the display begins to flash to put the display into calibration mode b While the decimal points are flashing press the or arrow pad until the reading on the display matches that measured by the digital recording thermometer If the arrow pads are not presse...

Страница 10: ... Agilent Technologies Inc 2014 Revision A1 February 2014 G2545 90002 G2545 90002 www agilent com ...

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