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Chapter 1

Overview

13

The ADInstruments ML125 NIBP (Non-Invasive Blood Pressure) Controller

performs non-invasive blood pressure measurement on rats and mice using

specialised tail cuffs.

This Owner’s Guide covers the features of the NIBP Controller and its

operation with your ADInstruments PowerLab

®

and LabChart software.

1

Overview

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

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Содержание NIBP ML125 Series

Страница 1: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

Страница 2: ...NIBP Controller Owner s Guide Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 3: ...ademarks Apple Mac and Macintosh are registered trademarks of Apple Computer Inc Windows Windows 7 and Windows Vista are either registered trademarks or trademarks of Microsoft Corporation All other trademarks are the property of their respective owners Product ML125 NIBP Controller Document Number U ML125 OG 003C Part Number 4375 Copyright April 2014 ADInstruments Pty Ltd Unit 13 22 Lexington Dri...

Страница 4: ...mHg Switch 16 Gain Adjust 16 Trigger In Connector 16 Trigger Out Connector 16 2 Using the Controller 17 Connecting the NIBP Controller 18 Connecting to PowerLab 18 Optional Trigger Controls 18 Connecting to Other ADInstruments Front ends 19 Power up Test 19 Recording with the NIBP Controller 19 Connecting Pulse Transducers 20 Indirect Blood Pressure Measurements in Small Animals using Tail Cuffs 2...

Страница 5: ... 27 The Pulse Input Connector 28 B Specifications 29 Cycle Operation 29 Manual Start Stop Input 29 Remote Trigger Input 30 Trigger Output 30 Pressure Output Cuff Pressure 30 Pulse Input 30 Operating Requirements 30 Physical Configuration 30 Index 31 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 6: ...o product is intended for the prevention curing or alleviation of disease injury or handicap Where a product meets IEC 60601 1 it is under the principle that it is a more rigorous standard than other standards that could be chosen and it provides a high safety level for subjects and operators The choice to meet IEC 60601 1 is in no way to be interpreted to mean that a product is a medical device m...

Страница 7: ...nectors are suitable for connection to human subjects even when there is direct electrical connection to the heart Warning symbol The exclamation mark inside a triangle means that the supplied documentation must be consulted for operating cautionary or safety information before using the device Further information is available on request Bio Amp Safety Instructions The Bio Amp inputs displaying an...

Страница 8: ...of these devices Several points must be observed for safe operation of the Isolated Stimulator The Isolated Stimulator output must only be used with the supplied bar stimulus electrode The Isolated Stimulator output must not be used with individual physically separate stimulating electrodes Stimulation must not be applied across the chest or head Do not hold one electrode in each hand Always use a...

Страница 9: ...afety Instructions To achieve the optimal degree of subject and operator safety consideration should be given to the following guidelines when setting up a PowerLab system either as stand alone equipment or when using PowerLab equipment in conjunction with other equipment Failure to do so may compromise the inherent safety measures designed into PowerLab equipment The following guidelines are base...

Страница 10: ...followed The ambient environment such as the temperature and relative humidity of the system should be kept within the manufacturer s specified range or the isolation barrier may be compromised The entry of liquids into equipment may also compromise the isolation barrier If spillage occurs the manufacturer of the affected equipment should be contacted before using the equipment Many electrical sys...

Страница 11: ...ion or adjustment to ensure safe operation Internal diagnostic software performs system checks during power up and will report errors if a significant problem is found There is no need to open the instrument for inspection or maintenance and doing so within the warranty period will void the warranty Your PowerLab system can be periodically checked for basic safety by using an appropriate safety te...

Страница 12: ...micals Storage Conditions Temperature in the range 0 40 C Non condensing humidity in the range 0 95 Operating Conditions Temperature in the range 5 35 C Non condensing humidity in the range 0 90 Disposal Forward to recycling center or return to manufacturer Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 13: ...NIBP Controller Owner s Guide 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 14: ...e blood pressure measurement on rats and mice using specialised tail cuffs This Owner s Guide covers the features of the NIBP Controller and its operation with your ADInstruments PowerLab and LabChart software 1 Overview Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 15: ...werLab and that the PowerLab is properly connected to a power socket and is switched on Status Indicator The Status indicator light can be in one of three states Off Idle no measurement in progress Green Inflating cuff Orange Deflating cuff Cuff Pressure Connection This port has a luer lock fitting and is where the tail cuff is attached The NIBP system can be purchased with either a cuff suitable ...

Страница 16: ...stop the measurement cycle during either inflation or deflation push the button again This will stop the cycle and immediately deflate the cuff The Back Panel I2 C Input and Output Sockets The NIBP Controller is designed to receive power and control signals from an ADInstruments PowerLab These are supplied by an I2 C Bus a bus is simply information transmission circuitry such as cables and connect...

Страница 17: ...signal to suit the type of pulse transducer being used It will have been factory set and you should not find it necessary to make further adjustment The adjustment of this control is described in Chapter 2 Trigger In Connector This trigger connector provides a means to start and stop the NIBP measuring cycle from an external device that is providing a TTL compatible signal This input can be connec...

Страница 18: ...o problems This chapter also covers setting up the NIBP Controller in a typical system and calibrating the pressure signals IMPORTANT Always make sure that the PowerLab is turned off before you connect or disconnect the NIBP Controller Failure to do so may damage the PowerLab and or the NIBP Controller Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 19: ...ut socket on the back of the NIBP Controller to another of the analog inputs of the PowerLab Both these connections must be made to determine blood pressure Optional Trigger Controls The NIBP Controller has two control signal sockets on the rear panel The Trigger In is used to start an NIBP measurement cycle from an external source such as the PowerLab By connecting this input to either the analog...

Страница 20: ... and the Status indicator should be off Recording with the NIBP Controller Follow these steps to begin recording with the NIBP Controller 1 Once the NIBP Controller is connected to your PowerLab as described above and as shown in Figure 2 1 connect the pressure cuff to the Cuff connection on the front of the NIBP Controller and connect the pulse transducer to the Pulse Input connector Connect the ...

Страница 21: ...r quickly As the pressure drops the pulse signal will start to increase again Typical data is shown in Figure 2 3 Connecting Pulse Transducers The Pulse Input connector Figure 1 1 on the front panel is a 6 pin DIN type connector allowing attachment of the pulse transducer in the MLT125M mice or MLT125R rats Pulse Transducer and Pressure Cuff assemblies Your NIBP system will have been purchased wit...

Страница 22: ...urement particularly when carried out on a well habituated animal The major advantage of the PowerLab approach to this technique is that it provides an accurate and permanent record of the pulsatile data recorded from the tail during the measurement cycle It therefore makes it possible for scientists to develop protocols and detection algorithms that correlate with their experiment We do not offer...

Страница 23: ...ricted as much as possible since they cause animal movement It sometimes helps to cover the restraint cage with a cloth to reduce the impact of external stimuli 4 Temperature maintenance Warming rats and mice improves blood circulation in the tail and the signal to noise ratio in the recording Typically animals should be preheated to 28 30 C and maintained at that temperature during the test 5 Tai...

Страница 24: ...ail pulse measurements are particularly disruptive because they often occur at times coincident with actual measurements However it should be noted that the technique is dependent not on the amplitude of the pulse but rather its onset 9 Systolic measurement Systolic measurement can normally be made with relative ease Systolic blood pressure SBP occurs when the cuff pressure corresponds to the rest...

Страница 25: ... the tail pulse rate and to directly indicate the position of the systolic blood pressure point as shown in Figure 2 4 Figure 2 4 Measurement of tail pulse rate using the Rate function of Cyclic Measurements In Zoom View the cursor is used to display a data value at a specific time point the value of the selected channel is displayed above along with the time point Artisan Technology Group Quality...

Страница 26: ...terial to use the NIBP Controller It is likely to be of interest to the technically minded and is not intended in any way as a service guide It should be noted that any modification or attempt to service your NIBP Controller voids your rights under the warranty A Technical Details Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 27: ...sure can be determined Circuit Principles To understand the internal operation of the NIBP Controller refer to Figure A 1 which shows a block diagram of the system Cuff Slow release valve Bandpass filter Pressure Signal Out Pulse Signal Out Pulse Input Pressure Transducer Microprocessor Pump Internal cuff Start Stop button Status indicator Trigger Out Trigger In Pulse Range switch Quick release va...

Страница 28: ...ring In order to determine the systolic pressure the NIBP system provides two analog output signals for animal pulse and cuff pressure An internal pressure transducer is used to monitor the air pressure in the cuff air supply line The output of the transducer is amplified and then provided as a signal from the rear panel on the controller The NIBP Controller is factory calibrated to produce 1 volt...

Страница 29: ...ired For transducers that produce very low level signals such as those used for rats or mice the high gain differential inputs are used pins 4 and 5 The output signal from the transducer should not exceed 250 μV The output of the transducer should be connected between the high gain input and high gain input pins To minimise noise pins 1 and 2 should be shorted together For high output pulse transd...

Страница 30: ...Operation Indication Trigger output normally low but high during inflation and deflation cycle Front panel Status indicator shows inflation and deflation operation Operation Abort The cycle can be terminated at any point by pressing the front panel Start Stop button again Pressure is automatically released Fast Release Time 1 2 s from 280 to 40 mmHg at 40 150 BPM 0 5 s from 280 to 40 mmHg at highe...

Страница 31: ...tion High 5V output level during measurement cycle Otherwise zero Pressure Output Cuff Pressure Sensitivity 0 1 V 0 300 mmHg factory calibrated Frequency Response DC to 10 Hz Pulse Input Input Impedance Differential Input 10 GΩ Single ended Input 1 MΩ Input Signal Range High gain differential input 0 25 μV up to 0 75 μV depending on rear panel Gain Adj setting Low gain single ended input 0 50 mV u...

Страница 32: ...V 50 mA Operating Conditions 5 35 C 0 90 humidity non condensing Physical Configuration Dimensions h w d 65 mm 200 mm 275 mm 2 56 7 9 10 8 Weight 1 7 kg ADInstruments reserves the right to alter these specifications at any time Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 33: ...NIBP Controller Owner s Guide 32 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 34: ...f pressure switch 16 P Power indicator 14 power up testing 19 PowerLab connection 18 pressure and pulse monitoring 27 pressure output connection 16 Pulse Input connector 15 28 pulse input gain adjustment 16 pulse output connection 15 16 Pulse Range switch 15 pump control 27 S Safety Notes 5 11 Start Stop button 15 Status indicator 14 storage 10 T Trigger controls 18 trigger input connection 16 tri...

Страница 35: ...NIBP Controller Owner s Guide 34 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Страница 36: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

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