Pannoramic SCAN II 2.0 User’s Guide
5 Scanning modes and settings
Multilayer mode
•
Single layer mode:
If none of the filters are selected in the Filters table for multilayer
scanning, this mode is active and can be selected, and Multilayer mode turns inactive.
•
Extended focus:
By choosing the
Extended focus
option, the software selects the sharpest
image from each focus level for each image field, and combines them into one single image.
Therefore those parts that were blurry in auto/manual focus will also be sharp. This method
guarantees the maximum depth of sharpness, however, it reduces digitization speed.
•
Z-stack scan mode:
Both for Extended focus and
Z-Stack
scanning methods, you can
determine the desired number of focus levels with the
Focus levels
selection box. The
current focus value is the starting point. If you set
5
for example, one current, two focus
levels above and below FOVs are captured. Therefore, it is advised to choose an odd number
for the focus levels. Separate layers are created and stored this way, and can be opened with
Pannoramic Viewer. The Z-stack slide contains the extended focus layer.
Focus step size
determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are:
Number of focus levels
: 7;
Focus step size
: 5.
Figure 42 – Focus level and step size
Scan settings
•
Change filters after whole slide scanning
To optimize speed, filter change should take place after the digitization of the slide with one
channel.
•
Change filters at each field-of-view
Filters should be changed for each field of view if very precise colocalization is required, but
consider that this option is more time consuming.
June 4, 2018 - Rev. 2
3DHISTECH Ltd.
74(142)
For research and education uses only, not for use in diagnostic procedures.
