CHAPTER 1 - NOTES ON DEVICE SAFETY
LSM 880
User Interface
ZEISS
11/2014 V_05
000000-2071-466
35
10.5
Using the GaAsP NDD 2 Channels on Axio Examiner Stand for Multiphoton
Imaging
The GaAsP NDD 2 channels is used instead of a regular objective holder on the Axio Examiner stand. It is
equipped with two high sensitive GaAsP detectors.
The detectors are used to detect emission signals generated with multiphoton excitation.
It is equipped with a dichroic filter which separates the excitation light from the emission light and which
directs the emission light towards the two detectors.
It is also equipped with a special filter cube which holds two emission filters and one dichroic filter. This
filter cube separates and filters the emission signals right before the detectors. Both filter units are set
and controlled manually.
The module can be exchanged for other objective holders. The system needs to be shut down when
doing so.
Mounting the GaAsP NDD 2 channels to the
microscope stand
•
Move the rear side of the unit (Fig. 26/
2
)
sideways towards the guide rail for the
objective holders (Fig. 26/
1
).
•
Once clicked into the guide rail move the unit
upwards until it's stopped by the upper pin
(Fig. 26/
3
).
•
Fix the unit to the stand with the wing screw
(Fig. 27/
1
) on the left hand side.
•
Connect the detector unit to the electronics of
the microscope via the cable on the left hand
side (Fig. 27/
2
).
•
Make sure the power supply and LVDS cables
are connected to the system accordingly.
Do not leave any opening for filters
uncovered when working with the
system.
Do not grab into these openings or
insert any other device when the system
is in use, as you may damage the unit or
risk getting your fingers in the way of
the laser beam.
Fig. 26
Mounting the GaAsP NDD 2
channels to the microscope
Fig. 27
Connecting the GaAsP NDD 2
channels to the microscope