BEL Engineering INV100-FL User Manual Download

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Inverted Fluorescence

Microscope

INV100-FL

USER MANNUAL

This manual is written for Inverted Microscope INV100-FL. For safety and for exerting

the best performance, making you familiar wit

the instrumen

entirely, it is strongly

recommended that you rea

this manual carefully before using the micro

scop

Summary of Contents for INV100-FL

Page 1: ...NNUAL This manual is written for Inverted Microscope INV100 FL For safety and for exerting the best performance making you familiar with the instrument entirely it is strongly recommended that you read this manual carefully before using the microscope ...

Page 2: ...teps 6 3 Adjustment and opertation 11 3 1 Lamp adjustment for fluorescence observation 11 3 2 Lamp adjustment for biological observation 17 4 Microscope photography and video 19 4 1Microscope Photography 19 4 2 Microscope Video 20 5 Technical Specifications 21 5 1 Outfit 21 5 2 Objective Specification 23 6 Troubleshooting 24 ...

Page 3: ... setting of the voltage switch and the frequency switch on the rear of the power supply unit 7 Always use the power cord provided and make sure that the main switch is moved to O OFF before connecting the power cord plug to the wall outlet 8 To prevent any hazard always turn the main switch on the power supply unit to O OFF unplug the power cord plug from the mains outlet before replacing the burn...

Page 4: ...t to use organic solvents to clean the non optical component of the equipment To clean these use a lint free soft cloth lightly moistened with a diluted neutral detergent 3 Do not disassemble any part of the power supply unit as malfunction or damage may occur 4 In order not to impair the safety of the equipment replace the burner when the counter of power supply indicates 100 00 hours To prevent ...

Page 5: ...rast slider Lamp reflected fluorescence units Condenser Illumination bracket eyepiece Stage inserted plate Stage Body nosepiece Objectives 4 10 20 40 Fluorescence power supply Protection barrier B excitation G excitation l INV100 FL inverted fluorescence microscope ...

Page 6: ...lling be sure every components is clean do not score any parts or glass surface Keep well with the supplied hexagon wrench When changing the components you will need it again Condenser illumination unit Phase contrast slider Lamp house Eyepiece Trinocular tube Objectives Glass stage Power supply unit Mechanical ruler Body Stand Stage lengthen splint Power supply Lever Protection barrier ...

Page 7: ...the setscrew and insert the trinocular Viewing Head into the body correctly screw down with bolt 2 2 2 Installing eyepiece fig 2 Insert the eyepiece into tube until they are against 2 2 3 Installing condenser set fig3 Install the condenser into the right direction fig3 图 1 Fig 2 Fig 3 ...

Page 8: ...d so it needn t to adjust in the use process If the ring is not in the center you could adjust by the centering bolt The 10X 20X light ring is worked with the 10X 20X phase contrast objective while the opening is used for bright field A B A B Fig 5 Fig 6 Notices Requirement 1 temprature 0 40 max humidity 85 2 high temperature and moisture will damage instrument and affect performance 3 keep the in...

Page 9: ...e used in the inversed microscope is very sensitivity about dust Do cover all the unused holes with turret dust caps to prevent the dust and contamination entering inside When operating use the low magnification objective 4X or 10X to search and focus the specimen at first then replace the higher magnifications if necessary When replace the objective slowly turning the nosepiece until you hear cli...

Page 10: ... supply unit and make sure the cord is correctly connected Make sure that the main switch of the power supply is set to O OFF before connecting cables 3 Connect the power cord connector into connector on the power supply unit and make sure the cord is correctly connected Verify that the voltage and the frequency of the AC mains outlet match the setting of the voltage switch and the frequency switc...

Page 11: ...omfortable adjustment 1 Installing the stage lengthen splint First Screw the fixed bolt on to the splint then mount it on to the stage from right or left below screwing down it until it stay hard 2 Installing the mechanical ruler Please install the ruler like the way of the stage splint 2 2 11 Installing protection barrier glass plate lever fig 16 1 Install the protection barrier on the attachment...

Page 12: ...bout 10mimutes that is until the mercury vapor inside it has cooled down and condenser to liquid Ensure that the hour counter is reset to 000 00 after replacement of the burner And you can insert a thin object such as a mechanical pencil tip into the reset hole on the front panel of the power supply unit to press the internal switch 3 1 2 Function of button fig 1 Hour counter Ammeter Excitation bu...

Page 13: ...rd a clicked while in video or photography pulling out the lever until it reached the clicked position Light path selector lever Brightness proportion application Pushing in the lever until it reached the limit position 100 used for binocular observation Binocular observation Pulling out the lever until it reached the limit position 20 used for binocular observation and 80 used for video or photog...

Page 14: ...move the objective from the revolving nosepiece position and engage this position in the light path 5 Pull out the field iris diaphragm lever to close the iris diaphragm and push in the aperture iris diaphragm lever to open the iris diaphragm to the large limit 6 Switch the light shutter to O position to open the light path 7 Turn the collector adjusting knob to project the arc image on the center...

Page 15: ...ing sure that the lamp housing has cooled down A mercury burner seals high pressure gas inside If the burner is used beyond its service life stress may accumulate inside the burner and in the worst but very rare case the burner could explode After replacing with a new burner reset the hour counter be sure to press the reset switch until 000 00 is displayed ...

Page 16: ... diaphragm lever out until the diaphragm comes into the smallest state 3 Use the hexangular wrench to adjust the two field iris diaphragm centering screws alternately to move the image of the diaphragm to the center Fig 2 show the adjustment of diaphragm 4 Push in the field diaphragm lever to open the diaphragm As this makes slight deviation noticeable adjust the centering precisely 5 Enlarge the ...

Page 17: ... Selecting the appropriate color filters according your need it became more effective to observe or photography the specimen Especially we suggest using the LBD color filter which can compensate more neutral colors You could pile up a group of color filters to the filter holder if you ensure they are level and the whole thickness is less than 11mm Color filter meaning IF550 Single contrast color f...

Page 18: ... adjusted properly before leaving factory How to adjust the tension of the coarse focusing knobTurning the tension adjustment collar while revolving at the direction of the arrow in the figure the tension of the coarse focusing knob is increasing and if at the contra direction the tension will decline 3 2 3 The centering ring fig 15 16 Usually you needn t the operation of centering If necessary pl...

Page 19: ...ht ring center and the phase contrast center when changing the objective If having departure you ought to center again If the light ring is centering incorrectly you will fail to obtain the best viewing effect of the microscopy After removing or replacing a thick specimen the light ring and the phase contrast ring are likely to deviate each other which will result in a decline of the image contras...

Page 20: ...he locking bolt on the trinocular viewing tube and take out the dust cap 2 Remove the dust cover on the both ends of the video accessories and revolve the screw head end into the CCD CMOS port 3 Install the accessories into the tri through port and screw down the bolt 4 1 3 Focus fig 2 Doing a binocular observation at 20 brightness look the image on the video or the computer which connected with t...

Page 21: ...e 5 Plug the digital photo connected head into the photo tube then screw down the locking bolts Before connecting the camera and adapter remove the camera lens firstly then connect the lens port with the adapter Pay attention to the gate type please To avoid the disturbing from the ocular in the observation please place the viewer finder on the two sides of the microscope when installing the camer...

Page 22: ...inite plan long working distance objective 40 In finite plan phase contrast objective PH10 In finite plan phase contrast objective PH20 Objectives In finite plan phase contrast objective PH40 10 20 40 phase annulus plate Phase contrast slider 10 20 40 phase annulus plate Mechanical stage stage 160 250mm inserted plate Stage strenthenplate 70 180mm Mechanical ruler movement 120 78mm Reflected illum...

Page 23: ...4 F 50 at 40 104 F z Pollution Degree 2 refer to IEC60664 note standard outfit optional INV100 FL 5 2 Objective Specifications TYPE MAGNIFICATION NUMERICAL APERTURE N A WORKING DISTANCE mm CONJUGATE DISTANCE mm FOCUS DISTANCE mm COVER SLIP THICKNESS 4X 0 1 25 2 45 Infinite Long Working Distance Plan Achromatic Objective 40X 0 6 3 2 45 1 2mm 10X 0 25 11 45 0 17 Infinite Long Working Distance Plan P...

Page 24: ...he located position Turn the nosepiece into the position where you can hear clicked 4 the color filter is stopped midway Insert deeply 5 2 The edge of the field of view has shadow or the brightness is asymmetry The phase contrast slider is not located in the proper position Turn the slider into the clicked position 13 There are stains on the specimen Change a clean specimen 3 Find dust and stain i...

Page 25: ... phase contrast observation Please use the compatible objective 12 the phase contrast effect When looking at the edge of the culture dish the phase contrast ring and the light ring is deviated each other Moving the culture dish until you obtain the phase contrast effect You also could demount the slider and dail the field diaphragm with the direction of 13 The nosepiece is not in the center of the...

Page 26: ...specified lamp Use the required lamp 3 Not use a appointed lamp use a appointed lamp 3 1 The height of the brightness is not enough The brightness adjustment knob is used wrong Adjust the brightness adjustment knob in a correct way 8 The bulb is going to spoil Change the bulb 3 2 The light glimpse The power cord have a poor contact Check the power cord and connect them exactly 6 Ⅳ Viewing tube The...

Page 27: ...0 There is faintness around the image It is a inherent character of the achromatic objective The problem is unavoidable if you used an achromatic objective 3 The indoor window or the fluorescence lamp develop The extra light entered into the eyepiece and viewfinder is reflected Cover up the eyepiece and the viewfinder of the microscope illumination system ...

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