Zeiss LSM 800, Standard Operation Protocol

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Imaging and Flow Cytometry Core CPOS LSM 800 SOP B-3
ver. 2.1 (Updated: 11/13/2020)
A. Overview
This guide will cover operation of the Carl Zeiss GmbH LSM 800 scanner paired with an inverted microscope
setup and ZEN Blue edition ver. 2.3 driving software. For specific operations of the ZEN Blue software please
read http://med.hku.hk/corefac/downloads/ZEN_BLUE2.3SOP_20200415.pdf . It is recommended that user study
both guides side-by-side.
B. Microscope Lens list
Position 1 2 3 4 5 6
Magnification 10x 20x 40 63 EMPTY 5x
Numerical Aperture 0.3 0.8 1.4 1.4 0.16
Free Working Distance
[mm] 2 mm 0.55 mm 0.13 mm 0.19 mm 18.5 mm
Coverglass Thickness
[mm] 0.17 mm 0.17 mm 0.17 mm 0.17 mm 0.17 mm
Immersion Air Air Oil Oil Air
Diagram showing arrangement of objective positions. Position 5 is reserved for special application lens thus will not
be populated at all times.