Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
N-25
ver. 2.1 (Updated: 11/13/2020)
N.
Oil immersion Lens clean procedure
Pre-requisite: you should know how to clean objective upon novice training. Seek technical support if you ae not
sure.
•
[N.B.] Always take lens tissue one by one from the original case. Discard any lens tissue in contact with
other surfaces (e.g. bench / air-table / microscope stage)
•
[N.B.] Use only lens cleaning tissue instead of Kimwipe or any coarser material to prevent scratches on
optical surfaces.
•
[N.B.] Quote Carl Zeiss Cleaning guide "The natural tendency to minimize waste is misdirected,
considering the relative cost of lens tissue compared to the potential of damaging an expensive
objective."
1.
Fold a piece of lens tissue into a long strip.
[insert diagram]
2.
Drawn the folded lens paper across objective
lens surface ONCE ONLY.
3.
Move to a new clean area of the lens tissue.
Repeat last step. If you run out of space on the
lens tissue pick up another one from the
container.
4.
Repeat this process until oil residue is no longer
observed on used lens tissue.
5.
Water / Glycerol / Silicon objective (not installed
on LSM 800 routine use. request from technical
staff for objective lens list and assistance)
6.
Fold a piece of lens tissue into a long strip.
[insert diagram]
7.
Drawn the folded lens paper across objective
lens surface ONCE ONLY.
8.
Move to a new clean area of the lens tissue.
Repeat last step until you run out of space from
the lens tissue.
