Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
F-10
ver. 2.1 (Updated: 11/13/2020)
25.
'Digital Offset' for mathematically subtract
apparent intensity of image typical setting = 0.
All pixel values will appear as: (original value) -
(Digital Offset value) = image pixel intensity.
26.
Go to 'Acquisition Mode' window:
27.
Adjust 'Frame Size' if you are not sure and do not
mind performing 2X Nyquist sampling, click
'optimal' option.
28.
Adjust 'Scan Speed' recommended value from
(slowest) ~4 µsec to (fastest) ~1 µsec 'Pixel
Dwell'
29.
Switch between scan directions unidirectional
[insert image] for quality; bi-directional [insert
img] for speed.
30.
Switch between bits per pixel [N.B.: different
degrees of intensities a pixel can represent] 8-
bits for presentation, representative images,
reports for faster work-flow; 16-bits for intensity
quantification / segmentation if required.
31.
Adjust 'Scanner rotation' angle if applicable
32.
Adjust 'Zoom' if applicable. [N.B.] This function
scans a smaller area through objective lens,
consult 'optimal' frame size after adjusting this
parameter.
33.
After all parameter has been set up do the
following to acquire an image.
34.
Ensure again all channels you need to acquire is
checked in 'Channels' window. (Refer to step
F15)
35.
Click 'New'
36.
Click 'Snap'
F27
F28
F29
F31
F32
F30
F35
F36
