Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
O-26
ver. 2.1 (Updated: 11/13/2020)
O.
System Power down
Prerequisite:
You have cleaned immersion objective lenses according to previous chapter [N].
1.
[Optional] if you have turned on live cell
incubator follow these steps.
2.
Turn off CO2 supply by turning gas tank valve
clockwise.
3.
Press 'Load Position' on Microscope control
panel.
4.
Wear gloves.
5.
Remove your sample from incubator holder.
Deposit into L6-11 live cell incubator if you wish
to re-culture the cells.
6.
Remove residual water from water bath with M-
fold paper towel.
7.
Take out incubator unit from microscope stage
GENTLY.
Do not use excessive force to avoid
hitting condenser with incubator.
8.
Use a kimwipe moistened with 70% Ethanol
(available on logbook station) to clean the
sample holder.
9.
Remove lens heater from objective lens.
10.
Turn off incubator controller. [8]
11.
-
12.
Press 'Load position' on microscope control
panel and then press 'Set as work position'
13.
on microscope control panel select lowest
magnification available (usually either a 5X or a
10X objective).
14.
Turn off ZEN software. If you are prompted with
save files window, check that all files on the list
is saved. If so, click 'unselect all' and then 'ok'.
15.
Turn off computer.
16.
Turn off [6] HXP 120 fluorescent lamp.
