6
4. Find the specimen
(Axioplan 2)
The
light path select
,
focus knob
and
stage
are manual controls. The rest of the microscope
is controlled by the software.
To set up the Axiovert microscope to locate your
specimen first move the
light path selector
to
VIS.
Move the correct objective in to place.
Do not use air-objectives after an
immersion objective without wiping the
immersion liquid (water or oil) from the
specimen and objective.
Brightfield, halogen light can be switched on and off
HAL
button
on the body of the microscope.
The intensity of brightfield light is most easily adjusted with the
Intensity control knob
on the body of the microscope
Arc lamp light can be shuttered on and off by either the
FL
button
on the body of the microscope on the right hand-side.
The
fluorescence filter
block carousel can be switched back
and forward using the two buttons on the body of the microscope,
underneath the left hand focus
knob.
Once you have found your specimen, pull the
light path
selector
out to the LSM position. The microscope will
automatically change the filter-position and shutter the light
sources. If you fail to do this you will receive an error when you
try to acquire a confocal image.
