2
Table of Contents
1.
START HARDWARE....................................................................................................................... 3
2.
START SOFTWARE........................................................................................................................ 4
3.
START LASERS ............................................................................................................................. 5
4.
FIND THE SPECIMEN
(AXIOPLAN 2)
..................................................................................................... 6
4.
FIND THE SPECIMEN
(AXIOVERT 200M)
................................................................................................. 7
5.
CONFOCAL FILTER SET CONFIGURATION.................................................................................. 8
6.
ACQUIRE PRELIMINARY CONFOCAL IMAGE............................................................................... 9
7.
OPTIMIZING THE SETTINGS........................................................................................................ 10
7.1.
G
ETTING THE DISPLAY READY
.................................................................................................... 10
7.2.
S
CAN CONTROL
:
C
HANNELS WINDOW
......................................................................................... 12
7.3.
A
CQUIRING YOUR FINAL IMAGE
.................................................................................................. 15
8.
SAVING YOUR FINAL IMAGE ...................................................................................................... 17
9.
ACQUIRING A Z-SERIES.............................................................................................................. 18
10.
ADVANCED OPTIONS ............................................................................................................. 20
10.1.
S
INGLE
-T
RACK
–
SIMULTANEOUS ACQUISITION
............................................................................ 20
10.1.1.
Getting the display ready ............................................................................................. 20
10.1.2.
Optimise settings ......................................................................................................... 20
10.2.
Z-
ATTENUATION COMPENSATION
................................................................................................ 21
10.3.
T
RANSMITTED LIGHT IMAGE
....................................................................................................... 22
10.4.
FRAP ................................................................................................................................... 23
11.
SHUTTING DOWN THE SYSTEM............................................................................................. 25
11.1.
T
URN OFF LASERS
................................................................................................................... 25
11.2.
R
EMOVE
S
PECIMEN AND CLEAN MICROSCOPE
.............................................................................. 25
11.3.
E
XIT THE SOFTWARE
................................................................................................................ 25
11.4.
P
OWER DOWN THE SYSTEM
....................................................................................................... 25
12.
OPENING YOUR IMAGES OFFLINE ........................................................................................ 26
12.1.
Z
EISS IMAGE BROWSER
............................................................................................................ 26
12.2.
I
MAGE
J ................................................................................................................................. 26
