22
DIC
BF
DF
Ph
10.3.
Transmitted light image
Whilst the laser is scanning the field of view a
certain amount of the excitation laser light passes
through the specimen. This light can be detected
and its intensity in each part of the field of view
corresponds to the transmitted light optical
properties of the specimen. In this way a
“brightfield” transmitted image can be
reconstructed. It is not a “confocal” image in that
the light does not come from a single plane.
DIC – differential interference contrast
BF – bright field
DF – dark field
For this, the microscope transmitted path needs to
be set correctly. Open the
Microscope Control
panel (Toolbar
Acquire
then
Micro
)
The
Field Stop
iris needs to be fully opened –
100%.
The
Filter
at 100%
The transmitted light to be at 0%.
Click o the
Condensor
button to reveal the
condensor options
The
Condensor Filter
needs to be set to the
appropriate type of brightfield i.e. BF, DIC,
DF or Ph.
NOTE: The software keeps changing the
condensor settings back to “BF”. Keep
checking that it is set correctly.
Then, whilst in fast scan mode, adjust the transmitted light channel’s (ChD) detector gain
and offset so that the background of the image is mid-grey and the brightest part of the
image is near saturation (i.e. shows red with the
Range Indicator
palette).
