Axiotech and Axiotech
vario
Carl Zeiss
B 40-020 e 06/99
3-17
3.4.5
Setting of epi-fluorescence
The epi-fluorescence technique
permits high-
contrast images of fluorescent substances
in
typical fluorescence colors. In the epi-fluorescence
microscope, the light (3-15/
1
) generated by a high-
performance illuminator reaches the excitation
filter ( 3-15/
A
) (bandpass)
via a heat-reflecting
filter. The filtered, short-wave excitation emission
(3-15/
2
) is reflected from a dichroic beam splitter
(3-15/
B
) and focused on the specimen via the
objective. The specimen absorbs the short-wave
emission and then emits long-wave fluorescence
(3-15/
3
) (
STOKES
’s law) which is now gathered by
the objective and transmitted by the dichroic beam
splitter ( 3-15/
B
). Finally, the beams pass a barrier
filter ( 3-15/
C
) (longpass) which allows only the
long-wave emission (3-15/
3
) from the specimen to
be transmitted.
The spectrum of excitation and barrier filters,
which are positioned in the 3 FL reflector slider
together with the appropriate dichroic
beam
splitter, must be matched very precisely.
The epi-fluorescence technique is only described for the Axiotech 100 and the Axiotech
vario
100, since
only these models permit attachment of different illuminators for fluorescence.
The following components are required for epi-fluorescence:
•
Filter slider FL with two mounts for dia. 25 mm filters, excitation filter, barrier filter, or
•
3 FL reflector slider, excitation filter, barrier filter.
The HBO 50 illuminator can also be attached, if requested.
The halogen illuminators 6 V 30 W or 12 V 100 W can also be used for strongly fluorescent specimens.
The following measures and settings are required for epi-fluorescence.
Fig. 3-15
Epi-fluorescence
Summary of Contents for Axiotech
Page 1: ...Axiotech and Axiotechvario Materials Microscopes Operating Manual...
Page 14: ...Carl Zeiss Axiotech and Axiotechvario XIV B 40 020 e 06 99...
Page 16: ...Carl Zeiss Axiotech and Axiotechvario 1 2 B 40 020 e 06 99...
Page 112: ...Carl Zeiss Axiotech and Axiotechvario 3 46 B 40 020 e 06 99...
Page 114: ...Carl Zeiss Axiotech and Axiotechvario 4 2 B 40 020 e 06 99...
Page 124: ...Carl Zeiss Axiotech and Axiotechvario A 2 B 40 020 e 06 99...
