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Sondes 

 

Section 2 

YSI Incorporated 

Environmental Monitoring Systems Operations Manual 

2-11 

6600EDS V2-2 SONDE BULKHEAD 

5 Port Sonde: 1 Rapid Pulse DO, 1 Conductivity/Temperature, 2 Optical, 1 pH/ORP 

 

  Figure 20B 

 

6562 Dissolved oxygen probe = 3-pin connector 

 

6560 Conductivity/Temperature = 6-pin connector 

 

6561 or 6561FG pH probe = 4 pin connector 

 

6565 or 6565FG pH/ORP probe = 4 pin connector 

 

6566 Fouling Resistant pH/ORP probe = 4 pin connector 

 

6026 Turbidity Probe, Wiping = 8 pin connector 

 

6136 Turbidity Probe, Wiping = 8 pin connector 

 

6025 Chlorophyll Probe, Wiping = 8 pin connector 

 

6130 Rhodamine WT Probe, Wiping = 8 pin connector 

 

6150 Optical Dissolved oxygen probe = 8 pin connector 

 

6131 PC-Blue-green Algae probe = 8 pin connector 

 

6132 PE-Blue-green Algae probe = 8 pin connector 

 

 

 

 
 
 
 
 
6600V2-4 SONDE BULKHEAD 

6 Port Sonde: 1 Conductivity/Temperature, 4 Optical, 1 pH/ORP 

  

  Figure 20C 

  

 

6560 Conductivity/Temperature = 6-pin connector 

 

6561 or 6561FG pH probe = 4 pin connector 

 

6565 or 6565FG pH/ORP probe = 4 pin connector 

 

6566 Fouling Resistant pH/ORP probe = 4 pin connector 

 

6026 Turbidity Probe, Wiping = 8 pin connector 

 

6136 Turbidity Probe, Wiping = 8 pin connector 

 

6025 Chlorophyll Probe, Wiping = 8 pin connector 

 

6130 Rhodamine WT Probe, Wiping = 8 pin connector 

 

6150 Optical Dissolved oxygen probe = 8 pin connector 

 

6131 PC-Blue-green Algae probe = 8 pin connector 

 

6132 PE-Blue-green Algae probe = 8 pin connector 

 

 

 

Cond/Temp – 6 pin 

pH/ORP – 4 pin 

 Cond//Temp – 6 pin 

pH/ORP – 4 pin 

 Rapid Pulse DO – 3 pin 

 

Summary of Contents for 6 series

Page 1: ...6 Series Multiparameter Water Quality Sondes User Manual 6 Series 6600 V2 6600EDS V2 6920 V2 6820 V2 600 OMS V2 600XL 600XLM 600LS 600R 600QS ...

Page 2: ... found in Appendix H EMC Performance A similar document for YSI Model 650 is located at the end of Section 3 650 MDS SPECIFICATIONS For general specifications for all YSI Environmental Monitoring Systems products included in this manual please see Appendix O Specifications GENERAL SAFETY CONSIDERATIONS For Health and Safety issues concerning the use of the calibration solutions with the sondes ple...

Page 3: ...E 3 21 3 5 LOGGING DATA WITH THE 650 3 27 3 6 MANAGING 650 FILES 3 46 3 7 UPLOADING DATA FROM SONDES 3 51 3 8 USING GPS WITH 650 3 51 3 9 USING THE 650 BAROMETER 3 53 3 10 UPGRADING 650 SOFTWARE 3 55 3 11 TROUBLESHOOTING 3 56 3 12 FERRITE BEAD INSTALLATION 3 57 3 13 SAFETY CONSIDERATIONS 3 58 3 14 650 MDS SPECIFICATIONS 3 61 SECTION 4 ECOWATCH FOR WINDOWS 4 1 INTRODUCTION 4 1 4 2 DATA ACQUISITION ...

Page 4: ... 1 LIMITATIONS OF WARRANTY 9 1 9 2 AUTHORIZED SERVICE CENTERS 9 2 9 3 CLEANING INSTRUCTIONS 9 2 9 4 PACKING INSTRUCTIONS 9 2 9 5 PRODUCT RETURN FORM 9 4 APPENDIX A HEALTH AND SAFETY A 1 APPENDIX B REQUIRED NOTICE B 1 APPENDIX C ACCESSORIES AND CALIBRATION STANDARDS C 1 APPENDIX D SOLUBILITY AND PRESSURE ALTITUDE TABLES D 1 APPENDIX E TURBIDITY MEASUREMENTS E 1 APPENDIX F FLOW F 1 APPENDIX G USING ...

Page 5: ...ce pollution Today we have thousands of instruments in the field that operate with the push of a button store data in memory and communicate with computers These instruments described in this manual are ideal for profiling and monitoring water conditions in industrial and wastewater effluents lakes rivers wetlands estuaries coastal waters and monitoring wells If the instrument has on board battery...

Page 6: ... assistance See Section 9 Warranty and Service Information for contact information 1 3 UNPACKING AND INSPECTION Inspect the outside of the shipping box for damage If any damage is detected contact your shipping carrier immediately Remove the equipment from the shipping box Some parts or supplies are loose in the shipping box so check the packing material carefully Check off all of the items on the...

Page 7: ...following list contains parameters that your sonde may measure See Appendix O Specifications for the specific parameters of each sonde Rapid Pulse Polarographic Dissolved Oxygen ROX Optical Dissolved Oxygen Conductivity Specific Conductance Salinity Total Dissolved Solids Resistivity Temperature pH ORP Depth Level Flow Turbidity Chlorophyll Rhodamine WT Phycocyanin Containing Blue green Algae Phyc...

Page 8: ...ion devices and VT 100 terminal emulators To utilize the configuration that will work best for your application make sure that you have all of the components that are necessary The following list and diagrams Figures 1 4 are a few possible configurations Sonde to Lab Computer recommended for initial setup Sonde to Data Collection Platform Sonde to Portable Computer Sonde to YSI 650 MDS Display Log...

Page 9: ...l Monitoring Systems Operations Manual 2 3 Figure 2 Sonde to Data Collection Platform You will need Sonde Field Cable 6096 Adapter with leads Data Collection Platform 6096 MS 8 Adapter with Flying Leads DCP Field Cable MS 8 Sonde YSI 692 0 Figure 3 ...

Page 10: ...s Operations Manual 2 4 Figure 4 Sonde to 650 Display Logger You will need Sonde Field Cable 650 MDS Display Logger YSI 650 operates on C cells or rechargeable batteries 650 MDS 610 DM Envir onmental Monitoring Systems YS Field Cable Sonde YS I 69 20 YS I 69 20 MS 8 ...

Page 11: ... DISSOLVED OXYGEN MEMBRANE Note If you are using a ROX Optical DO sensor for your applications please skip to Section 2 3 2 at this time The 6562 Rapid Pulse Polarographic DO probe is shipped with a protective dry membrane on the sensor tip held in place by an O ring Remove the O ring and membrane Handle the probe with care It is very important not to scratch or contaminate the sensor tip See Sect...

Page 12: ... touching it only at the ends With the thumb and forefinger of your right hand grasp the free end of the membrane With one continuous motion gently stretch it up over and down the other side of the sensor The membrane should conform to the face of the sensor Secure the end of the membrane under the forefinger of your left hand Roll the O ring over the end of the probe being careful not to touch th...

Page 13: ...supplied place electrolyte on the DO probe tip until a high meniscus is formed as shown in Figure 8 below Figure 8 Figure 9 Hold the membrane so that all four corners are supported but do not stretch the membrane laterally Position the membrane over the probe keeping it parallel to the probe face as shown in Figure 9 above Using one continuous downward motion stretch the membrane over the probe fa...

Page 14: ...een done properly the finished product should have no bubbles wrinkles or tears as shown in Figure 13 above NOTE Observe the following cautions to assure that your membrane installation is proper Secure the sonde tightly so that it will not move during membrane installation Wash hands before installation and do not allow finger oils or O ring lubricant to touch the probe face or the membrane Use c...

Page 15: ... 15 16 and 17 illustrate the uses of the common tool for port plug removal Note that this tool will also be used to install the various probes If the tool is misplaced or lost you may use 7 64 and 9 64 hex keys as substitutes Figure 16 Figure 17 Figure 18 NOTE You may need pliers to remove the ISE port plugs but do not use pliers to tighten the ISE probes Hand tighten only Now refer to Figures 19 ...

Page 16: ...ature 6 pin connector 6561 or 6561FG pH probe 4 pin connector 6565 or 6565FG pH ORP probe 4 pin connector 6566 Fouling Resistant pH ORP probe 4 pin connector 6882 Chloride Probe leaf spring connector 6883 Ammonium Probe leaf spring connector 6884 Nitrate Probe leaf spring connector 6026 Turbidity Probe Wiping 8 pin connector 6136 Turbidity Probe Wiping 8 pin connector 6025 Chlorophyll Probe Wiping...

Page 17: ...tical Dissolved oxygen probe 8 pin connector 6131 PC Blue green Algae probe 8 pin connector 6132 PE Blue green Algae probe 8 pin connector 6600V2 4 SONDE BULKHEAD 6 Port Sonde 1 Conductivity Temperature 4 Optical 1 pH ORP Figure 20C 6560 Conductivity Temperature 6 pin connector 6561 or 6561FG pH probe 4 pin connector 6565 or 6565FG pH ORP probe 4 pin connector 6566 Fouling Resistant pH ORP probe 4...

Page 18: ...Dissolved oxygen probe 8 pin connector 6131 PC Blue green Algae probe 8 pin connector 6132 PE Blue green Algae probe 8 pin connector 6820V2 2 6920V2 2 SONDE BULKHEADS 5 Port Sonde 1 Conductivity Temperature 2 Optical 1 pH ORP 1 ISE Figure 21B 6560 Conductivity Temperature 6 pin connector 6561 or 6561FG pH probe 4 pin connector 6565 or 6565FG pH ORP probe 4 pin connector 6566 Fouling Resistant pH O...

Page 19: ... 600R BULKHEAD Figure 22 If are working with a 600R sonde your instrument will arrive with the probes installed 600QS BULKHEAD Figure 23 If are working with a 600QS sonde your instrument will arrive with the probes installed TEMPERATURE pH GLASS pH REFERENCE 6850 CONDUCTIVITY DISSOLVED OXYGEN TEMPERATURE pH GLASS ORP pH REFERENCE 6850 CONDUCTIVITY DISSOLVED OXYGEN Conductivity Temperature Depth ...

Page 20: ... ring lubricant supplied in the YSI 6570 Maintenance Kit to the O rings on the connector side of each probe that is to be installed Figure 25 CAUTION Make sure that there are NO contaminants between the O ring and the probe Contaminants that are present under the O ring may cause the O ring to leak when the sonde is deployed NOTE Before installing any probe into the sonde bulkhead be sure that the...

Page 21: ...2 2 6600EDS V2 2 6820V2 2 and 6920V2 2 sondes can accept and utilize two of the six optical sensors at the same time The two optical ports of these sondes are labeled T and C on the sonde bulkhead Each port can accept any of the six sensors so be sure to remember which sensor was installed in which port so that you will later be able to set up the sonde software correctly The 6600V2 4 sonde can ac...

Page 22: ...n the sonde bulkhead This will ensure that connector seals will not allow leakage INSTALLING THE PROBE GUARD Included with each sonde is a probe guard The probe guard protects the probes during calibration and measurement procedures Once the probes are installed install this guard by aligning it with the threads on the bulkhead and turn the guard clockwise until secure CAUTION Be careful not to da...

Page 23: ...eir specific locale The circuit board in this instrument contains a manganese dioxide lithium coin cell battery that must be in place for continuity of power to memory devices on the board This battery is not user serviceable or replaceable When appropriate an authorized YSI service center will remove this battery and properly dispose of it per service and repair policies POWER FOR LAB CALIBRATION...

Page 24: ... paying special attention to polarity Labeling on the battery compartment posts describes the orientation It is usually easiest to insert the negative end of battery first and then pop the positive terminal into place Figure 34 Check the O ring and sealing surfaces for any contaminants that could interfere with the O ring seal of the battery chamber CAUTION Make sure that there are NO contaminants...

Page 25: ...correct polarity before installing the batteries into the battery chamber CAUTION Be sure the orange O ring is installed in the groove of the lid The o ring is designed with retention tabs to prevent it from falling out of the grove during installation Check the O ring and sealing surfaces for any contaminates which could interfere with the O ring seal of the battery chamber Remove any contaminate...

Page 26: ...profile of the groove allowing the center tab to be seated properly You may need to use the tip of one of your thumbs to finish pressing the tab into place Figures 35G and 35H 5 Press the remaining middle retaining tab into the groove as before and verify that none of the other retaining tabs have been forced from their positions in the groove during this part of the seal installation Figures 35I ...

Page 27: ...ed upside down or that one of the cells is not at full strength CAUTION Remove Batteries When Not in Use As with any battery powered instrumentation batteries should be removed before short or long term storage Even with the new battery lid batteries can leak releasing toxic and corrosive battery acid and damaging equipment INSTALLING BATTERIES IN THE 6920V2 1 AND 6920V2 2 SONDES To install the 8 ...

Page 28: ...ctor remove the waterproof cap from the sonde connector and set it aside for later reassembly during deployment or storage Then connect your field cable to the sonde connector A built in key will ensure proper pin alignment Rotate the cable gently until the key engages and then tighten the connectors together by rotating clockwise Attach the strain relief connector to the sonde bail Rotate the str...

Page 29: ...ring Systems Operations Manual 2 23 CAUTION The 6067B cable is for laboratory use only it is not waterproof and should not be submersed Sondes that are equipped with level sensors use vented cables See Appendix G Using Vented Level for detailed information ...

Page 30: ...t the prompt Press Enter or click on OK and the display will indicate that EcoWatch is proceeding with the setup routine Simply follow the instructions on the screen as the installation proceeds 2 4 2 RUNNING ECOWATCH FOR WINDOWS To run EcoWatch for Windows simply select the EcoWatch icon on your desktop or from the Windows Program Menu For help with the EcoWatch program see Section 4 EcoWatch or ...

Page 31: ...p to the next section If you wish to change the language of your menus to German or French use the following instructions Follow the step by step instructions below to change the language for the menus in your 6 series sonde Connect your sonde to the serial port of a PC with access to the Internet using the proper cable as described in the previous section of this manual Make sure that the sonde i...

Page 32: ...right corner of the window Your sonde menus will now appear in the language which you selected prior to running the updater If you want to change the language associated with your sonde menu you MUST rerun the YSI Code Updater and select the new language via this mechanism INTERFACING TO THE SONDE WITH ECOWATCH FOR WINDOWS When you select Sonde from the EcoWatch for Windows menus the PC based soft...

Page 33: ...le is properly connected If you are using external power make certain that the YSI 6651 or 6038 power supply or other 12 vdc source is properly working Recheck the setup of the Com port and other software parameters Also refer to Section 6 Troubleshooting The sonde software is menu driven You select functions by typing their corresponding numbers You do not need to press Enter after choosing a sel...

Page 34: ...0 Select option 0 for previous menu 2 Upload Sonde SONDE MENU FLOW CHART 2 Unattended sample 1 Conductivity 2 DO 1 Run 3 File 2 Calibrate 6 Report 7 Sensor 8 Advanced 1 Discrete sample 3 DO mg L 2 Time 3 Temp C 4 Temp F 1 Date 2 Cond 3 DO 4 ISE1 pH 1 Temp 4 Others 2 Comm Setup 3 Page Length 4 Instrument ID 5 System 1 Date Time 5 SDI 12 Address 3 Quick Upload 4 View File 1 Directory 5 Quick View Fi...

Page 35: ... 12 30 Select option 0 for previous menu Select 4 Instrument ID from the System setup menu to record the instrument ID number usually the instrument serial number and press Enter A prompt will appear which will allow you to type in the serial number of your sonde This will make sure that any data that is collected is associated with a particular sonde Note that the selection 5 Circuit Board SN sho...

Page 36: ... PC 6132 BGA PE or 6150 ROX Optical DO installed as indicated by the submenus NOTE CAREFULLY It is NOT possible to simultaneously activate BOTH the 6562 Rapid Pulse polarographic dissolved oxygen sensor and the 6150 ROX Optical dissolved oxygen sensor Activation of either sensor will automatically deactivate the other selection Thus users of 6600V2 2 6600EDS V2 2 6820V2 1 and 6920V2 1 sondes CANNO...

Page 37: ...bidity would be presented as turbid NTU This designation is designed to differentiate the data from the two sensor types in later analysis For parameters with multiple unit options such as temperature conductivity specific conductance resistivity and TDS a submenu will appear as shown below for temperature allowing selection of desired units for this parameter Select units 1 NONE 2 Temp C 3 Temp F...

Page 38: ...leep RS232 should usually be off For sondes that will be used in unattended monitoring studies Auto sleep RS232 should usually be on This is described in detail in Section 2 9 Sonde Menu When this setup is verified press Esc or 0 to return to the Advanced menu Select 3 Sensor from the Advanced menu and make certain that the entries are identical to those shown below Advanced 1 Cal constants 2 Setu...

Page 39: ...alled in your unit For example if you do not have a chlorophyll probe the last two entries which are relevant only to chlorophyll will not appear When this setup is verified press Esc or 0 to return to the Advanced menu For a detailed explanation of the choices in the Advanced menu see Section 2 9 8 Advanced Press Esc or 0 to back up to the Main menu The sonde software is now set up and ready to c...

Page 40: ...re has convex bottoms Insure that all sensors are immersed in calibration solutions Many of the calibrations factor in readings from other probes e g temperature probe The top vent hole of the conductivity sensor must also be immersed during calibrations CALIBRATION TIPS 1 If you use the Calibration Cup for calibration of either the Rapid Pulse Polarographic or ROX Optical DO sensors in water satu...

Page 41: ...e damage to the threaded portions of the bottom cap and tube Remove the probe guard if it is installed Inspect the installed gasket on the sonde for obvious defects and if necessary replace it with the extra gasket supplied Screw the cup assembly into place on the threaded end of sonde and securely tighten Note Do not over tighten as this could cause damage to the threaded portions of the bottom c...

Page 42: ...e Upright Inverted Conductivity 320ml 150ml pH ORP 240ml 175ml ISE 240ml 175ml All Optical Sensors 225ml DO NOT CALIBRATE Table 2 6820V2 2 and 6920V2 2 Sondes with Extended Calibration Cup Probe to Calibrate Upright Inverted Conductivity 310ml 150ml pH ORP 200ml 150ml ISE 200ml 150ml All Optical Sensors 225 ml DO NOT CALIBRATE Table 3 600XL and 600XLM Sondes Probe to Calibrate Upright Inverted Con...

Page 43: ...ted using either the calibration cup supplied with the sonde or with an extended length calibration cup which can be purchased as an option for the 6820V2 1 and 6920V2 1 An extended cup is supplied as a standard item with the 6820V2 2 6920V2 2 6600V2 4 6600V2 2 and 6600EDSV2 2 sondes If you choose to calibrate with the short calibration cup you also MUST first make certain that the vessel is equip...

Page 44: ...sure to read the instructions carefully before using the sonde 2 6 2 CALIBRATION PROCEDURES The following calibration procedures are the most commonly used methods for the 6 series sensors For detailed information on all calibration procedures refer to Section 2 9 2 Calibrate To ensure more accurate results you can rinse the calibration cup with water and then rinse with a small amount of the cali...

Page 45: ...ext calibration CALIBRATION EXAMPLE The example below for calibration of specific conductance is designed to demonstrate the general calibration protocol for all parameters From the Calibrate menu press 1 Conductivity and the following display will be shown Select 1 SpCond the generally recommended method for calibration of a conductivity sensor and the following prompt will appear which calls for...

Page 46: ...SI 3163 is recommended into a clean dry or pre rinsed calibration cup Before proceeding ensure that the sensor is as dry as possible Ideally rinse the conductivity sensor with a small amount of standard that can be discarded Be certain that you avoid cross contamination of standard solutions with other solutions Make certain that there are no salt deposits around the oxygen and pH ORP probes parti...

Page 47: ...water saturated and for the temperature to equilibrate Two calibration protocols are provided below for Rapid Pulse dissolved oxygen one for sampling applications and one for long term monitoring applications Sampling Applications If your instrument will be used in sampling applications where the dissolved oxygen is on continuously during the study deactivate Autosleep RS232 as described in Sectio...

Page 48: ...ter the current barometric pressure in mm of Hg Inches of Hg x 25 4 mm Hg Note Laboratory barometer readings are usually true uncorrected values of air pressure and can be used as is for oxygen calibration Weather service readings are usually not true i e they are corrected to sea level and therefore cannot be used until they are uncorrected An approximate formula for this uncorrection where the B...

Page 49: ...ature should be entered for maximum accuracy For example the pH of YSI pH 7 Buffer is 7 00 at 25 C but 7 02 at 20 C After entering the correct pH value of the buffer press Enter and the current values of all enabled sensors will appear on the screen and change with time as they stabilize in the solution Observe the readings under pH and when they show no significant change for approximately 30 sec...

Page 50: ... no significant change for approximately 30 seconds press Enter After the first calibration point is complete proceed as instructed on the screen to continue Rinse the sonde in water and dry the sonde prior to the next step Place the proper amount of 1 mg L standard for Ammonium or Nitrate 10 mg l for Chloride into a clean dry or pre rinsed transport cup Carefully immerse the probe end of the sond...

Page 51: ...com STANDARDS FROM OTHER VENDORS ARE NOT APPROVED FOR THE YSI TURBIDITY SYSTEM AND THEIR USE WILL LIKELY RESULT IN BOTH CALIBRATION ERRORS AND INCORRECT FIELD READINGS For AMCO AEPA standards the value entered by the user during the calibration protocol is DIFFERENT depending on which YSI turbidity sensor Legacy 6026 or Current 6136 is being calibrated This reflects the empirically determined fact...

Page 52: ...in chlorophyll from site to site or over time at a single site To increase the accuracy of your chlorophyll measurements follow the 2 point or 3 point calibration protocols outlined in Section 2 9 Sonde Menu Before making any field readings carefully read Section 5 14 Chlorophyll and Appendix I Chlorophyll Measurements that describe practical aspects of fluorescence measurements To begin the calib...

Page 53: ... and use the default sensitivity for calculation of phycoerythrin containing BGA in cells mL allowing quick and easy fluorescence measurements that are only semi quantitative with regard to BGA PE However the readings will reflect changes in BGA PE from site to site or over time at a single site To increase the accuracy of your BGA PE measurements follow the 2 point calibration protocols outlined ...

Page 54: ...ave stabilized Activate the wiper 1 2 times by pressing 3 Clean Optics as shown on the screen to remove any bubbles After stabilization is complete press Enter to confirm the first calibration and then as instructed press Enter to continue Dry the sonde carefully and then place the sonde in the second rhodamine WT standard 100 ug L is recommended using the same container as for the 0 ug L standard...

Page 55: ...nected and internal battery power used to operate the sonde Alternatively the sonde may connect via SDI 12 communication to a data collection platform DCP Sondes without batteries need to be connected to an external power source for unattended sampling Select 1 Run from the Main menu to begin taking readings or to set verify the parameters required for a study There are two options in the Run menu...

Page 56: ...onde memory and the following message will be displayed Sample logged By entering 2 LOG ON OFF a set of data can be logged to memory and the following message will be displayed LOG is ON hit 2 to turn it OFF 3 Clean optics Press 2 again to terminate logging By entering 3 Clean optics if your unit has an optical probe the wiper will clean the optical surface The 3 Clean optics portion of the prompt...

Page 57: ...art the instrument at the site at 6 00 PM as noted in the above example change the Start Time to 18 00 00 Select 4 Duration and set the length of the study in days The default value is 365 days which is longer than most deployments In most cases you will either want to stop the unattended study manually or allow the batteries to be expended It is wise to set the duration to a value longer than the...

Page 58: ...onde by uploading all existing data from the sonde memory to your PC and then deleting them out of the sonde see 3 File from Main Menu You may want to change the batteries for longer battery life You can lengthen the sampling interval to extend both battery life and memory capacity Review the screen below which now displays the entries made above and reflects your logging conditions Once you press...

Page 59: ...n the duration you specified has expired or the batteries are expended If you want to terminate sooner simply select 2 Unattended sample from the Run menu then B Stop logging Select 1 yes and return to the Unattended setup menu Logging 1 Interval 00 15 00 2 Next at 07 17 96 3 Next at 18 00 00 4 Stop at 07 31 96 5 Stop at 18 00 00 6 File clrlake3 7 Site Clear Lake at Spillway 8 Bat volts 11 7 9 Bat...

Page 60: ...t close EcoWatch for Windows 5 Open the Real Time menu click on New and select the location where you want to data transferred Name the file making sure that the name has extension RT The default location for the file is in the Data subdirectory of the ECOWWIN directory 6 Click OK After EcoWatch sets up the sonde for the study data transfer will begin at the sample rate you selected The data will ...

Page 61: ...e file in the Directory Select 2 Upload to view file lists in memory and upload the data to PC based software Prior to upload a Time window display appears that will allow you to select portions of the logged data to upload You may select 1 Proceed to upload all logged data from the dates and times displayed Filename Samples 1 BRIDGE1 dat 19 2 BRIDGE2 dat 27 3 UPLAKE dat 33 4 CLRLAKE2 dat 167 5 DW...

Page 62: ...A subdirectory Select 4 View File to examine the data in any file currently stored in the sondes flash disk memory You will first view the same screen as viewed in the Directory menu From this menu choose the file of interest then using the Time window menu choose the dates and or times of interest If you choose dates or times that are not within the designated start and stop times no data will be...

Page 63: ...ftware For the purposes of describing and demonstrating EcoWatch plotting reporting and data manipulation capabilities we use the file SAMPLE DAT available on the compact disk that was enclosed with your 6 series sonde This file will be copied to your hard drive during EcoWatch installation and should be available to you for the instructions below When you are ready to move beyond the example SAMP...

Page 64: ...et of menu items in the top line Some of these functions are specifically related to viewing and manipulating data Next we will examine some of the viewing options VIEWING DATA To look at some of the viewing options click on View Figure 42 Note that the Toolbar and Status Bar are turned on check mark In addition the 4 Digit Year expression is checked Also note that a check mark is just left of the...

Page 65: ... Figure 42 Viewing Options It may be somewhat awkward to scan the data table in this manner therefore you have the option to turn off the graphical representation and allow the table to fill the window See Figure 43 Notice now that when you click on View the Graph item is no longer checked ...

Page 66: ...n for each parameter activated The Study function provides useful information about the design of the study including sample interval date time number of samples sensor identification and parameters reported The Statistics and Study windows may be activated over either graph or table presentations To view either of these windows click on View highlight the desired function and click again The wind...

Page 67: ...along with specific data values in boxes to the left of the displayed graphs as shown in Figure 45 You can hold down the right mouse button and move the mouse to scan the entire graph that is displayed in the window The values in the boxes change as you move the mouse This feature is very useful for quantifying specific data without the need to open the data table and scroll through what may be th...

Page 68: ...arameters are viewed you may add and delete parameters you may change plot appearance using different interval times and different units and you may change the x axis if you prefer a parameter other than date or time The Top Line menu selection that allows you to select some of these parameter changes is Setup Click on Setup then Parameters From here there are four submenus that allow you to Add R...

Page 69: ...set of axes For display of table formatted results you may change font font style size and text color In addition you may change table color and highlight color The menu structure is easy to follow Try some changes to gain familiarity with these Setup display options CHANGING DISPLAY FORMATS USING GRAPH FUNCTION The top line menu labeled Graph as the name suggests can be used to examine critical e...

Page 70: ...s Below in Figure 47 you see some of these functions One very commonly used function is Limit Data Set If you choose this function by clicking on the highlighted item as shown below you then use the mouse to move your cursor to the left limit of an area of interest click once then move the mouse to the right limit of interest and click again The result will be a close up look at the specific area ...

Page 71: ... section SAVE IMPORT EXPORT AND PRINT COMMANDS Under File function in the top line menu you can save a particular presentation version of a data file and give the presentation a customized name For example you might want to call the expanded plot in Figure 48 DIURNAL using the File Save Data Display command This presentation will be saved with your data file and can be recalled in further by using...

Page 72: ...nd add original data back Under the same Parameters function we have selected Attributes and changed the Average Interval from the default 0 to 60 Since data was collected every 15 minutes the change to a 60 minute interval helps to smooth out the graph and average out any short term noise events Next we again select Setup then Graph From the functions available we first selected Title Page and ty...

Page 73: ...and gave the custom plot the name 4PARAM to that the presentation can be immediately recalled in the future As you become more familiar with EcoWatch for Windows the plotting analysis and reporting functions can be accomplished easily and quickly Practice with all of the functions and again do not forget to use Window s Help for more detail or see Section 4 EcoWatch for Windows ...

Page 74: ...e menus select the sonde icon in EcoWatch for Windows and press Enter See Figure 40 above for the Sonde Menu Flow Chart In the following subsections you will learn about the functions of the various menu items and when to use them The discussion of the menu and submenu functions is organized in numerical order beginning with Section 2 9 1 Run IMPORTANT MESSAGE WHAT IF THERE IS NO RESPONSE TO A KEY...

Page 75: ...ld be used in the vertical profiling of a lake or river where the user lowers the sonde incrementally into the water and data acquired for a few minutes at each depth In this section you will learn how to use the sonde menu structure to open and close files set the interval between samples start the sampling operation and log data to the internal memory of the sonde Select 1 Discrete sample from t...

Page 76: ...represents the number of seconds between samples The maximum sample interval is 32767 seconds 9 hours The factory default sample interval is 4 seconds and works best for most discrete sampling applications The shortest possible sample interval for obtaining new DO readings is 4 seconds If a smaller interval is selected then the DO readings remain constant for the number of samples necessary to fil...

Page 77: ...ns and you want to restart the file with a different name press 5 Close file and rename the file Select 4 Site to assign a site name with a maximum of 31 characters This allows you to enter the name of the site where you are sampling When you select 5 Open File a file is opened and the number 5 changes to Close File When you are finished logging data to the file press 5 Close File and designation ...

Page 78: ... change the Start Time to 18 00 00 Select 4 Duration and set the length of the study in days The default value is 365 days which is longer than most deployments In most cases you will either want to stop the unattended study manually or allow the batteries to be expended It is wise to set the duration to a value longer than the anticipated deployment If you cannot retrieve the sonde at the expecte...

Page 79: ...hese parameters to your data file be certain that they are active in the Report setup before you begin the unattended study After making the above entries the sonde software will automatically estimate the expected battery life and the time it will take for the sonde memory to be filled This information is displayed on the screen for your consideration If the battery life or the free memory capaci...

Page 80: ...be shown on the screen as they are stored to internal memory The Unattended study will terminate when the duration you specified has expired or the batteries are expended If you want to terminate sooner simply select 2 Unattended sample from the Run menu then B Stop logging Select 1 Yes and return to the Unattended setup menu Logging 1 Interval 00 15 00 2 Next at 07 17 96 3 Next at 18 00 00 4 Stop...

Page 81: ...continuously no matter what sample interval has been selected Under these conditions the user retains manual control of the dissolved oxygen calibration routine viewing the stabilization of the readings in real time and confirming the calibration with keyboard entries If Auto sleep is activated the sonde will warm up the sensors for the period of time selected for the DO sensor Under these conditi...

Page 82: ...tion mode be certain that the sensor has been thermally equilibrated in water saturated air and that the sensor shows stable readings prior to beginning the calibration routine particularly after a membrane change Note however that the sensor should be off for at least 5 minutes before initiating a calibration procedure with Autosleep active If possible it is ideal that the sensor be off for a tim...

Page 83: ...ration Then as instructed press Enter again to return to the Calibrate menu Note that at calibration the DOsat percent air saturation value will reflect the barometer value that was entered For example if a barometric pressure of 720 mm is entered the DOsat value will change to 94 7 720 760 x 100 Note that the parameter DOsat Local is also available for users who prefer to set their percent air sa...

Page 84: ...l the user elects to perform a 2 point calibration as described below at which time it will change slightly to reflect the adjustment of the zero oxygen value You will also note that a different set of constants for your specific sensor membrane are supplied on the instruction sheet which came with your 6150 probe These five constants K1 K4 and C have a different appearance than those shown in Adv...

Page 85: ...g Percent Air Saturation 1 Point NOTE YSI recommends that you use this method for calibration of your 6150 Dissolved Oxygen Sensor to obtain the maximum accuracy under normal operating conditions Place the sensor either a into a calibration cup containing about 1 8 inch of water which is vented by loosening the threads or b into a container of water which is being continuously sparged with an aqua...

Page 86: ... known concentration of oxygen within 10 of air saturation if you select ODO mg L These two point calibrations should be done ONLY if you suspect that your 6150 Optical DO sensor is less accurate than you require at low oxygen values The key to performing a 2 point calibration is to make certain that your zero oxygen medium probably either nitrogen gas or an aqueous solution of sodium sulfite at a...

Page 87: ...to 100 no matter what barometer value is entered The method of activating this parameter its use and its limitations are described in Appendix J of this manual The key factor to remember however is that no matter which convention ODOsat or ODOsat Local is selected the mg L value will not be affected PRESSURE ABS AND GAGE Select Pressure Abs non vented or Gage vented to zero the depth sensor The de...

Page 88: ... a pond is known to vary between 5 5 and 7 a two point calibration with pH 7 and pH 4 buffers is appropriate Three point calibration with an additional pH 10 buffer will not increase the accuracy of this measurement since the pH is not within this higher range To begin the calibration immerse the sonde in one of the buffers and enter the actual pH value depending on your calibration temperature Pr...

Page 89: ... concentration value again press Enter and view the stabilization of the values on the screen in real time After the readings have stabilized press Enter to confirm the calibration Then as instructed press any key to return to the Calibrate menu Select the 3 point option to calibrate the NH4 probe using three calibration solutions two at ambient temperature and one at a temperature significantly d...

Page 90: ...om STANDARDS FROM OTHER VENDORS ARE NOT APPROVED FOR THE YSI TURBIDITY SYSTEM AND THEIR USE WILL LIKELY RESULT IN BOTH CALIBRATION ERRORS AND INCORRECT FIELD READINGS For AMCO AEPA standards the value entered by the user during the calibration protocol is DIFFERENT depending on which sensor 6026 or 6136 is being calibrated This reflects the empirically determined fact that 6026 and 6136 sensors th...

Page 91: ...instructed press any key to return to the Calibrate menu Select the 3 point option for maximum accuracy over the entire range of 0 to 1000 NTU As for the 2 point procedure one of the standards must be 0 NTU Because of the linearity characteristics of the sensors we recommend that the other two standards have turbidity values of 100 and 1000 NTU However the user can select any values that are deeme...

Page 92: ... low in chlorophyll an appropriate choice of standards might be 0 and 10 µg L However for general purpose measurements an appropriate choice of standards is usually approximately 100 µg L To begin the calibration immerse the sonde in the 0 µg L standard as instructed and press Enter It is mandatory that the 0 µg L standard be calibrated first The screen will display real time readings that will al...

Page 93: ... input the correct value in cells mL press Enter and view the stabilization of the values on the screen in real time After the readings have stabilized press Enter to confirm the calibration Then as instructed press any key to return to the Calibrate menu For all BGA PC calibration procedures be certain that the standard and sensor are thermally equilibrated prior to proceeding with the calibratio...

Page 94: ...een fluorescence and actual BGA PE readings and is designed primarily to evaluate the sensor for drift See Section 5 17 Principles of Operation for more information on the proper calibration of your chlorophyll sensor OPTIC RHODAMINE WT When selecting Rhodamine there will be a choice of 1 point 2 point or 3 point calibrations for your rhodamine WT sensor The 1 point option is normally used to zero...

Page 95: ...ny parameter follow the instructions below Activate any calibration screen 1 point 2 point or 3 point where a numerical entry is requested In the example below a 2 point turbidity calibration was selected Instead of entering a numeric value type the word uncal and press Enter An uncal entry at any calibration prompt will reset both slope and offset for this parameter to the factory default setting...

Page 96: ...The file will have a glp extension and will have the Circuit Board Serial as the default filename The file can be viewed by following the path File Directory View from the Main sonde menu File details 1 View file 2 File 00003001 glp 3 Samples 39 4 Bytes 790 Filename Samples 1 BRIDGE1 dat 19 2 BRIDGE2 dat 27 3 UPLAKE dat 33 4 CLRLAKE2 dat 167 5 DWNLAKE2 dat 31 6 00003001 glp 39 File 1 Directory 4 V...

Page 97: ...rb M1 500 0000 08 23 2001 17 07 50 00003001 Turb A2 1000 000 08 23 2001 17 07 50 00003001 Turb M2 1000 000 08 23 2001 17 07 50 00003001 Chl Offset 0 000000 08 23 2001 17 07 50 00003001 Chl A1 100 0000 08 23 2001 17 07 50 00003001 Chl M1 100 0000 08 23 2001 17 07 50 00003001 Chl A2 200 0000 08 23 2001 17 07 50 00003001 Chl M2 200 0000 08 23 2001 17 07 50 00003001 Fluor Offset 0 000000 08 23 2001 17...

Page 98: ...f varying sizes To examine the details of each file press the associated number e g 4 for CLRLAKE2 dat and an additional screen will be displayed as shown below that quantifies the time of the deployment the sample interval and the site where the sonde was used In addition the data in the file can be viewed by using the 1 View file command Select 2 Upload to view file lists in memory same as shown...

Page 99: ... allow you the option to select portions of the logged data to upload You may select 1 Proceed to upload all data logged from the dates and times shown Select 1 Proceed Choose the appropriate file transfer protocol A status box will appear in the lower right quadrant of the screen Verification of a successful transfer is indicated when all of the requested data are transferred Press 0 or Esc to re...

Page 100: ... will not be deleted The management of the glp calibration record file that is automatically stored in sonde memory and is described in Section 2 9 2 above is similar to that for data files However there are some differences in the upload procedure of which the user should be aware When uploading a glp file there will be a choice of three upload protocols as shown below The binary upload choice sh...

Page 101: ...en In options 4 5 and 6 you can view battery voltage battery life and available free memory in your sonde to help you evaluate whether the current setup is appropriate to complete an active logging or to begin a study in which you have defined your logging parameters If you press key 4 5 or 6 before you exit this screen it may change since this triggers the Status screen to update information Note...

Page 102: ... Select option 0 for previous menu Select Date time Date time setup 1 m d y 4 4 digit year 2 d m y 5 Date 08 11 98 3 y m d 6 Time 11 12 30 Select option 0 for previous menu Press 4 and 5 to activate the date and time functions Pay particular attention to the date format that you have chosen when entering date Use the 24 hour clock format for entering time Option 4 4 digit year may be chosen to hav...

Page 103: ... Select 4 Instrument ID from the System setup menu to record the instrument ID number usually the instrument serial number and press Enter A prompt will appear which will allow you to type in the serial number of your sonde This will make sure that any data that is collected is associated with a particular sonde The 5 Circuit Board SN entry shows the serial number of the PCB that is resident in yo...

Page 104: ...nit options such as temperature conductivity specific conductance resistivity and TDS a submenu will appear as shown below allowing selection of desired units for this parameter Select units 1 NONE 2 Temp C 3 Temp F 4 Temp K Select option 0 for previous menu 2 After configuring your display with the desired parameters press Esc or 0 to return to the Main menu Even if all of the sensors are enabled...

Page 105: ...Dissolved oxygen in air saturation set to 100 at calibration from the Rapid Pulse sensor DO mg L Dissolved oxygen in milligrams per liter from Rapid Pulse Sensor DO chrg Dissolved oxygen sensor charge from Rapid Pulse Sensor ODOsat Dissolved oxygen in air saturation from ROX Optical Sensor ODOsat Local Dissolved oxygen in air saturation set to 100 at calibration from the ROX Optical Sensor ODO mg ...

Page 106: ...selection In this example the time temperature conductivity dissolved oxygen pH ORP and battery sensors are enabled To disable a sensor simply press the number of the active sensor or port and the asterisk will disappear For the ISE and Optic selections press the appropriate number and then enable or disable the sensor using the submenu choices Be certain that the appropriate sensor is enabled in ...

Page 107: ...tware will produce submenus that allow the proper optical sensor to be selected Note however that only one probe of any type can be installed and activated in sondes with multiple optical ports For example it is not possible to use chlorophyll probes in both Optic T and Optic C ports of the 6600V2 2 NOTE CAREFULLY It is NOT possible to simultaneously activate BOTH the 6562 Rapid Pulse polarographi...

Page 108: ... N A ODO regression coefficient ODO K4 N A N A ODO regression coefficient Pres offset if not vented 14 7 20 7 to 8 7 if vented 0 0 6 to 6 mV offset 0 0 100 to 100 pH offset 0 0 400 to 400 pH gain 5 0583 6 07 to 4 22 NH4 J 51 2 Not checked NH4 S 0 195 0 15 to 0 217 NH4 A 1 092 Not checked NO3 J 99 5 Not checked NO3 S 0 195 0 217 to 0 15 NO3 A 2 543 Not checked Cl J 99 5 Not checked Cl S 0 195 0 217...

Page 109: ...to the factory default From the Advanced menu select 2 Setup to display miscellaneous options Type the appropriate number to activate deactivate any of the displayed features 1 VT100 emulation Activate this option for VT100 terminal emulation This feature allows the sonde to send escape sequences to clear the screen which in turn results in an improved display Usually this feature should be activa...

Page 110: ...ecommended except for special applications 5 Start up NMEA When this item is active the sonde will start sampling and output data in a format which is compatible with devices which utilize the NMEA protocol See Appendix N NMEA Applications for additional details Activation of this item is NOT recommended except for special applications 3 Comma radix When this item is enabled the sonde will replace...

Page 111: ...ion the interval in the Run Unattended sample mode will also change to the Sample and Hold interval In this case however you will be able to change the Unattended Sample interval but with the caveat that the Sample and Hold interval will also change i e the Unattended sample interval and the Sample and Hold interval MUST be synchronized and the software assures that this will be the case For DCP a...

Page 112: ...nity This item is not used or displayed if the pressure sensor is enabled in the Sensors menu Latitude 40 This selection allows you to input the global position latitude where the sonde is sampling The units are degrees For accuracy enter the decimal equivalent to indicate degrees and minutes For example enter 41o 30 as 41 5 This value is used in the calculation of depth or level to account for gl...

Page 113: ...he sonde is forced to wait for the Rapid Pulse DO warm up time to expire before displaying any readings Note that in SDI12 mode or while calibrating the Rapid Pulse DO sensor the warm up time is used regardless of the activation of this item Disabling this item allows you to see data without having to wait during the Rapid Pulse DO warm up time Under normal operating conditions this item should be...

Page 114: ...les across the probe optics just at the time a reading is taken Activation of this option generally results in a better display of the average turbidity of the water under examination and its use is recommended for most sampling and unattended applications This item will only appear if a turbidity sensor is enabled in the Sensors menu Chl temp co C 0 0 This entry sets the coefficient for the tempe...

Page 115: ...12 rhodamine WT 12 chlorophyll 12 BGA PC 24 BGA PE 24 ODO 12 and other 4 Note that the time constant can be set independently for each optical probe and that time constant choices are only available for activated sensors Rhodamine WT would appear in the display below if it had been activated as a sensor All other sensors use the same time constant as shown below Data filter setup 1 Enabled 2 Wait ...

Page 116: ...s will be filtered again For the purposes of the filter consecutive readings are never more than 0 5 seconds apart When sampling faster than 0 5 seconds consecutive readings will be at the faster rate and you may want to adjust the threshold accordingly See section 2 9 1 for details on fast sampling The threshold feature is intended to speed response to large changes in a reading For example when ...

Page 117: ...he proper procedure for storage of the sensors that will maximize their lifetime and minimize the time required to get the sonde ready for a new application This section will describe interim or short term storage between applications where the sonde is being used at a regular interval daily weekly biweekly etc and long term storage e g over the winter where the sonde will not be used on a regular...

Page 118: ...ical item from the YSI 6570 Maintenance Kit supplied with your sonde At the time of o ring replacement the entire o ring assembly should be cleaned as described below To remove the o rings Use a small flat bladed screwdriver or similar blunt tipped tool to remove the o ring from its groove Check the o ring and the groove for any excess grease or contamination If contamination is evident clean the ...

Page 119: ...rt the pressure cap supplied with the instrument should be securely tightened in place If moisture has entered the connector dry the connector completely using compressed air a clean cloth or paper towel Apply a very thin coat of lubricant from the 6570 Maintenance Kit to the O ring inside the connector cap before each installation 2 10 2 PROBE CARE AND MAINTENANCE Once the probes have been proper...

Page 120: ...r NO circumstances should you use organic solvents to clean your sensor membrane When the 6150 sensor is not in field use it MUST BE STORED IN A MOIST ENVIRONMENT i e either in water or in water saturated air with storage in water being preferable If the sensor membrane is allowed to dry out by exposure to ambient air it is likely to drift slightly at the beginning of your next deployment unless i...

Page 121: ...e has been shipped in a humidified atmosphere and the package SHOULD NOT BE OPENED until immediately before membrane replacement Once the sensor membrane has been installed on the 6150 DO sensor as described below it is important to keep the membrane in an environment which is characterized by 100 humidity Thus after replacement of the membrane and installation of the sensor system in your sonde t...

Page 122: ...o not heat the membrane assembly Make certain that there is an O ring in place in the groove surrounding the sensor cavity Place the sensor membrane assembly over the wiper shaft and align the holes in the membrane holder with those on the probe face so that the sensor cavity is located over the optical fiber ends on the probe face Be absolutely certain that the sensor cavity lines up with the pro...

Page 123: ...lean cloth lens cleaning tissue or cotton swab to remove all foreign material from the glass bulb 6561 and 6565 and platinum button 6561 Then use a moistened cotton swab to carefully remove any material that may be blocking the reference electrode junction of the sensor CAUTION When using a cotton swab with the 6561 or 6565 be careful NOT to wedge the swab tip between the guard and the glass senso...

Page 124: ...hole Continue flushing the pressure port until the water comes out clean CAUTION Never try to remove the circular pressure port cap For 6920V2 1 6920V2 2 6600V2 2 6820V2 1and 6600V2 2 sondes the depth sensor is exposed to the water by either a circular access port on the side of the sonde or a though hole on a module just above the sonde bulkhead A syringe is supplied in the maintenance kit to aid...

Page 125: ...than is necessary for a firm grip Use slipjaw pliers to minimize the chance of applying too much pressure on the module While the module is removed from the sonde be sure to dry the sonde port and probe connector with compressed air and apply a very thin coat of lubricant to the O ring before re installation NOTE The ion selective membranes are fragile Be certain to 1 Use only moist high quality l...

Page 126: ...ibration cup should be sealed to prevent evaporation The key for interim storage is to use a minimal amount of water so that the air in chamber remains at 100 percent humidity The water level has to be low enough so that none of the sensors are actually immersed Any type of water can be used in this protocol Distilled deionized or tap water If the storage water is inadvertently lost during field s...

Page 127: ...s it may damage the pH glass sensor that must remain in the sonde 600 with Combination pH Sensor Instruments of this design were generally sold prior to January 1996 and can be identified by the presence of only 3 probes temperature dissolved oxygen pH in the bulkhead Be certain that the dissolved oxygen sensor has an undamaged membrane and electrolyte in place Fill the provided storage vessel wit...

Page 128: ...s high enough to completely cover the DO sensor Seal the vessel to prevent evaporation of the water At the end of the storage time remove the existing membrane and re membrane the probe using new electrolyte 2 Remove the Rapid Pulse dissolved oxygen sensor from the sonde leaving the electrolyte and membrane in place Store the probes in water tap deionized or distilled are equally acceptable in a b...

Page 129: ...e dehydration and will require replacement It is also important to remember not to store the pH sensor in distilled or deionized water as the glass sensor may be damaged by exposure to this medium The long term storage protocol is dependent on the instrument For all YSI 6 series sondes other than the 600R and 600QS the recommended long term storage protocol is identical Remove the probe from the s...

Page 130: ...s provided and set aside in room air The chloride ISE sensor utilizes a solid state membrane that provides specificity For long term storage the module should be removed from the sonde wiped clean with moist lens cleaning tissue and placed in its storage boot to prevent abrasion TURBIDITY CHLORPHYLL BGA PC BGA PE AND RHODAMINE WT No special precautions are necessary for either the short or long te...

Page 131: ... with a YSI 6 Series sonde the 650 will allow the user to easily Display real time readings from YSI 6 series sondes Log real time sonde data to internal meter memory with custom site lists Calibrate 6 series sondes Set up 6 series sondes for deployment Upload data from sondes for transfer to PC 3 2 GETTING STARTED This section is designed to quickly familiarize you with the hardware and software ...

Page 132: ...6117 battery pack included 6127 Rechargeable Battery Pack Kit with 6116 charger adapter cable 6123 universal charger China Australia power cord and 6117 battery pack included 4654 Tripod 614 Ultra Clamp 5085 Hands Free Harness 5065 Form fitting Case 6117 Extra Rechargeable Battery Pack 6115 GPS Cable for interface with user supplied GPS unit 616 Cigarette Lighter Charger If you have ordered any of...

Page 133: ...if a file named TEST is uploaded initially with a memory allocation of 50 kB and then is uploaded at a later time and overwritten with a memory allocation of 100 kB the total memory occupied is 150 KB even though only one file with the designation TEST and a file size of 100 kB is shown in the file directory For this reason when dealing with large files the user may wish to transfer files to a PC ...

Page 134: ...data transfer or software upgrade You will need 650 6115 GPS cable User supplied GPS unit with DB 9 cable YSI 6 series sonde with integral or field cable 650 YSI 6 series sonde Integral or field cable User supplied GPS unit with DB 9 cable 6115 GPS cable You will need 650 YSI 655174 PC interface cable PC with active serial port 650 PC Serial port DB 9 655174 PC Interface Cable ...

Page 135: ...s described in detail in subsequent sections of this manual They are presented here so that your will be able to ascertain if you have all of the parts necessary for your applications You will need 650 with battery pack installed 6116 Charger adapter cable 6114 Wall charger or equivalent 6116 Charger adapter cable 6114 wall charger shown or 6123 power supply 650 ...

Page 136: ...s as shown in the diagram above There are four function keys up down right and left arrow keys and an alpha numeric keypad The top left key that has a green circle and line is the ON OFF key The top right key activates the display backlight The Escape key is labeled and is the Enter key Display Backlight Key On Off Key Cellular Phone Style Keypad Enter Key Escape Key MS 8 Connector Strain Relief L...

Page 137: ...RIES AND CHARGING The YSI 650 can be powered either with 4 alkaline C cells or a rechargeable NiMH battery pack With the C cell configuration the user will be able to power a typical YSI 6 series sonde active dissolved oxygen and one optical sensor for approximately 45 hours of continuous operation The rechargeable battery pack will allow for about 15 hours of continuous use under these conditions...

Page 138: ...tive screws on the battery lid and then remove the battery lid completely Insert the cells between the battery clips being sure to follow the polarity and as indicated on the bottom of the battery compartment Make certain that the gasket is properly installed on the battery lid before reinstallation Reinstall battery lid and tighten the 4 captive screws securely and evenly using a Phillips or slot...

Page 139: ...y 6 hours to get a full charge 3 Do not charge the batteries continuously for more than 48 hours 4 For long term storage keep your battery pack between the temperatures of 20 C and 30 C and remove it from the 650 case 5 Do not charge your batteries at temperatures below 0 C or above 40 C 6 Do not use or store the battery pack at high temperature such as in strong direct sunlight in cars during hot...

Page 140: ...vehicle s lighter socket The 616 cigarette lighter charger contains a 2 ampere fast blow type fuse If the power cord stops working properly unscrew the adapter s cap remove the tip and then pull out and check the fuse See diagram above to remove the fuse If the fuse is blown or you are unsure replace it with a new 2 amp fast blow fuse that is available at electronics stores such as Radio Shack Rea...

Page 141: ...ey which is located at the top right of the keypad The instrument backlight should illuminate the LCD so that the display can be read Pushing the backlight key again will turn the backlight off The backlight will power off automatically after two minutes of non use 3 2 7 ADJUSTING THE DISPLAY CONTRAST The contrast of the 650 display is automatically temperature compensated to provide a proper disp...

Page 142: ...ers for sonde sensors and changing entries such as DO warm up time and optical wiper interval in the Sonde Advanced Sensor submenu You will follow an example of this type of entry when you set the 650 clock in the example below When both numeric and alpha entry are appropriate for input the 650 keypad provides a cellular phone style interface for choosing the proper character As shown in the above...

Page 143: ...atically scroll to the right to prepare for the next alphanumeric input If you make a mistake in the entry simply return to the previous character with the left arrow key and reenter the number or letter After the entry is complete press the Enter key to confirm it You will follow an example of this type of entry when you enter an Instrument ID name for your 650 in the example below To familiarize...

Page 144: ...he following display Enter the proper number from the keypad for the first date digit Note that when you release the key the cursor moves automatically to the next number Repeat this process until the date is correct Then press Enter to confirm the Date entry Note also that as described above since alpha characters are inappropriate for this entry only numeric entry is possible Highlight the Time ...

Page 145: ...on to generate the number 2 as opposed to the previous clock setting example where a single press of the numeric 2 key generated the number 2 After completing the above example press the Escape key repeatedly to return to the 650 Main menu and then proceed to the next section 3 2 9 CONNECTING TO A SONDE The primary use of the 650 is to interface with YSI 6 series sondes In this configuration you w...

Page 146: ...e sonde Instructions for use of these windows are provided in subsequent sections of this manual Note also that there is a disk with rotating segments in the upper right corner of the display whose movement confirms that the 650 sonde interface is functional To return to the 650 Main menu press the Escape key CAUTION When using the 650 MDS in field applications which are in close proximity to comm...

Page 147: ...etup menu The value is can be used simply as a meteorological parameter or can be used in calibration of sonde dissolved oxygen sensors The barometer reading is NOT corrected to sea level and is updated in real time 2 10 4 Battery Capacity Status The graphic indicator shows the portion of the battery capacity that is remaining either for the 4 C cell configuration or for the optional rechargeable ...

Page 148: ...the upgrade procedures contact YSI Technical Support for advice After reading the above sections and participating in the simple examples provided you should be familiar with the basics of battery installation keypad and display function and keypad data entry Please proceed to Section 3 3 where the setup of your 650 is described in detail 3 3 SETTING UP THE 650 The 650 has a number of features whi...

Page 149: ...e life of your 650 batteries by deactivating this selection Note that the selection MUST BE OFF in order to determine the voltage of the internal batteries of sondes important information for Unattended studies 3 3 4 Baud rate to sonde This entry allows the user to adjust the baud rate for communication with the sonde It does not affect communication for upload of files to a PC Highlight the entry...

Page 150: ... can also obtain a barometric pressure value from your local weather service This latter value has usually been corrected to sea level and therefore must be uncorrected before it can be used in calibration of the 650 barometer See Section 3 9 of this manual for details Select Calibrate barometer and press Enter Input the known barometric pressure from the 650 keypad in the selected units mm Hg in ...

Page 151: ...me way that would be possible if the sonde were attached to a PC From the 650 Main Menu select Sonde menu and press Enter A display similar depending on sonde type and sonde software version to that shown below will appear Note that the icon in the lower right of the screen has changed from meter to sonde indicating that the menu shown originates in the 6 series sonde Note also that the 650 displa...

Page 152: ...e Activating Deactivating sensors Setting up the desired Report output Calibrating the sensors Changing the sample interval for Discrete Sample studies Performing file management functions on the memory in the sonde Checking the Status or System setup of the sonde Setting up sondes for Unattended sample deployments Setting up sondes for Discrete sample logging studies to sonde memory Uploading fil...

Page 153: ...d by continuing to scroll the highlighting block down with the arrow key After activation of salinity in the Report setup of the sonde press the Escape key twice in succession to return to the Main sonde menu Example 2 Calibrating the Dissolved Oxygen Sensor NOTE In the following example it is assumed that the Autosleep RS232 function of your sonde has been disabled in the sonde Advanced Setup men...

Page 154: ...etric pressure Then press the Enter key and observe the dissolved oxygen reading until stabilization occurs Make sure the Calibrate selection is highlighted in upper window NOTE The current barometric pressure as read by your 650 will automatically appear as the default value if your 650 is equipped with a barometer When the dissolved oxygen reading is stable press the Enter key to confirm the cal...

Page 155: ... sample rate can be set to higher values in the Discrete Sample menu This feature will be described in detail in Section 3 5 2 To Run the sonde from the Discrete sample menu highlight Start sampling and press the Enter key 3 4 3 2 Setting up a sonde for deployment Use the 650 Sonde menu selection to access Run Unattended sample and then proceed with the setup for the monitoring study as described ...

Page 156: ...nde Utilize the 650 Sonde menu selection to access the File menu of the sonde Highlight the View file selection and press Enter Select the file that you want to view Choose Proceed and press Enter to view the entire file or select the portion of the data that you want to view and then select Proceed and press Enter to confirm your selection After data has appeared on the screen use the arrow keys ...

Page 157: ... either as single points after the user has verified stabilization or as a continuous data stream at a fixed sample interval No matter where or how the user decides to log field data with a 650 sonde system the 650 software plays the key role in controlling the process When setting up the 650 sonde system for your logging application it important to realize that the 650 Run display from which all ...

Page 158: ...ighlighting cursor associated with the logging windows can be moved within a particular window using the up down arrows and toggled from window to window using the left right arrows Note also that if your sonde contains an optical sensor it is possible to activate the probe wiper prior to logging from the Sonde logging window While the following sections will show that the logging functions of the...

Page 159: ...5 2 1 INTRODUCTION All YSI 6 series sondes sold since September 1999 have memory present on their internal PCBs In addition all 600XLM 6920 and 6600 sondes have internal memory regardless of the date of purchase Interface of these sondes to a 650 allows the user to easily store data either a single point or a continuous stream to the sonde memory If a continuous stream of data is desired the inter...

Page 160: ... scroll horizontally to view all of the data The user manually records the data from the site and turns the system off 3 5 2 3 EXAMPLE 2 CONTINUOUS LOGGING APPLICATION TO SONDE MEMORY A user wants to carry out a vertical profile of a lake from 0 to 30 feet in order to detect the presence of a thermocline and if identified to locate its position in the water column The experiment will involve lower...

Page 161: ...ters the user uses the 650 keypad to change the Sample interval from 0 5 seconds to 16 seconds and inputs custom file PROFILE1 and site WEST LAKE designations The user then highlights Start sampling and lowers the instrument into the water to begin the study The data displayed on the screen is updated every 16 seconds The user then highlights the Start logging selection in the sonde logging window...

Page 162: ...ple interval is now stored in the sonde memory under the file name PROFILE1 It can be viewed on the 650 display and or uploaded to the 650 memory or directly to a PC by accessing the sonde File menu 3 5 2 4 SONDES WITHOUT MEMORY If your sonde is a 600R 600XL or 6820 purchased prior to September 1999 it may contain a circuit board that does not have memory With sondes of this type you will not be a...

Page 163: ...details and examples of the flexibility of logging sonde data to 650 memory The distinction between a simple occasional logging with file and site names entered at the logging site and b logging to a more sophisticated file structure where file parameters are entered into a site list prior to going to the field will be described 3 5 3 2 SIMPLE LOGGING TO 650 MEMORY The term simple logging to 650 m...

Page 164: ...d then press Esc to return to the 650 Main menu Once the sample interval is set correctly in the 650 Logging setup menu the user simply highlights the Sonde run selection in the 650 Main menu and presses Enter to begin data display The user places the sonde in the water and then highlights the Start logging selection in the 650 logging window upper left and presses Enter The user is then prompted ...

Page 165: ...se the arrow keys to scroll horizontally in order to view all of the data Note that using these same basic guidelines data could have been logged at another site on the same day with the user inputting a different site designation file name and site name in the field Data could also have been logged single points or as a continuous data stream at each site simply by the choice of Log single point ...

Page 166: ...e characterized primarily by the file name but will also have the Site name attached so that it is viewable in either the 650 File directory or in EcoWatch for Windows after upload to a PC Site Designations associated with applications where data from multiple sites are logged to a single file This type is referred to as a Multi site Designation and is characterized by three parameters a file name...

Page 167: ...gging studies at various field sites The following discussion and examples should help you understand how to set up site lists for various applications LOGGING OF DATA TO 650 MEMORY FROM SINGLE SITES TO SINGLE FILES USE OF SINGLE SITE DESIGNATIONS IN A SITE LIST The setup and use of Single Site Designations will be provided in the following application example Water quality data needs to be collec...

Page 168: ...splay depending on the position of the cursor To make Site list entries for this application begin by highlighting the Filename position pressing Enter and inputting the file name WEST in this case from the keypad Press Enter to confirm the entry and the cursor will automatically move to the right for the entry of a Site name Enter BLUE LAKE for the site and press Enter to confirm the entry The cu...

Page 169: ...E If you want to return to the Edit site list display for changes before logging use the RIGHT ARROW KEY to highlight the Configure selection and press Enter The up down arrows will only scroll within the Site list The data from the two locations has been logged to the selected files that can be either viewed manually using the 650 View file menu or uploaded to EcoWatch for Windows from the 650 Fi...

Page 170: ... screen similar to that for non site list files which will aid in diagnosing the differences in the parameter setup that is preventing the existing file from being appended with new data LOGGING OF DATA FROM SEVERAL DIFFERENT SITES TO THE SAME FILE USE OF A MULTI SITE LIST The setup and use of Multi Site Designations will be provided in the following example which deals with a different approach t...

Page 171: ... that will be available in the field for logging of data from two sites WEST and EAST to a single file BLUELAKE To use this list you need to first make certain that the entry Use site list is active in the 650 logging setup Then travel to the WEST site activate the 650 Run display and then select either a continuous data stream Start logging or a single data entry Log single point from the 650 log...

Page 172: ...hat can be either viewed manually using the 650 View file menu or uploaded to EcoWatch for Windows from the 650 File Upload to PC menu The Site Number 1 for the WEST site and 2 for the EAST site will be stored with each data point and will be thus be available for identifying where the data was taken The file BLUELAKE can be examined by use of the 650 File selection In the 650 File menu highlight ...

Page 173: ...ing the file BLUELAKE with additional data from these or other sites the logging setup sonde parameters barometer GPS must be identical to that for the initially stored data If there has been a change in setup the Site Designation will be grayed out in the Site List indicating a parameter mismatch 3 5 3 4 EDITING YOUR SITE LIST The 650 software provides powerful tools for editing your site list Ac...

Page 174: ...me is identical for several files To copy the Filename from the previous file to a new entry below simply highlight the new Filename block press Enter and then enter site names and site numbers The site list will show these blanks in the Edit site list mode as shown on the left below but when the site list is accessed for logging studies the actual Filename will appear to avoid confusion This is s...

Page 175: ... from the Run menu of the sonde Log continuous data stream data at long or short sample intervals up to 15 minutes to 650 memory using the Sample interval selection in the Logging setup menu of the 650 Log data from the 650 internal barometer to the memory of the 650 Log data from a user supplied GPS unit to the memory of the 650 as long as the GPS unit is set up in NMEA format and has the proper ...

Page 176: ...n deals with the management of data that have been placed in 650 memory either through direct logging from your sonde or from the upload of data stored in sonde memory A file by definition contains data and is distinct from the Site Designations in a Site list that are used to configure actual data files For example deleting an entry from the site list after points have been logged under this desi...

Page 177: ...rectory selection in the 650 File menu and press Enter to display the list of files resident in the 650 memory Note that the size of the file is shown in two different ways 1 the number of samples logged data points of several parameters and 2 the total number of bytes of memory occupied by the file Highlight the top file PROFILE1 and press Enter to generate a display of the details of this file P...

Page 178: ...information under Interval will identify whether the data were logged by manual control of the system Discrete or to the memory of a sonde set up in an Unattended sampling study Only for Unattended sampling studies will the actual sample interval be displayed When the Interval has the designation Discrete the time between samples can be determined by viewing the data as described below ...

Page 179: ...m EcoWatch in spreadsheet compatible form if the user prefers this method of data analysis and management In order to carry out the upload of data files to your PC you must first connect the 650 to a serial port of your computer via the 655174 PC Interface cable as shown in the following diagram After the connection is made run EcoWatch for Windows click on the sonde icon in the upper toolbar and ...

Page 180: ...her the sonde sensors or the 650 barometer For files with a dat or txt extension transfer to a PC using the Upload to PC command will proceed automatically and retain the file type that is present in 650 memory For files with a glp extension an additional screen will appear on activating the transfer that gives a choice of binary CDF or ASCII for moving the file to the computer You currently MUST ...

Page 181: ...mmarize the allocation of the used memory space In the example above 6400 bytes are used in defining the memory directory 512 bytes are taken up with accessible files and 0 bytes are taken up in deleted files As described in Section 3 2 2 above deleted files arise when the same file is uploaded from a sonde several times with the file overwritten There is a lot of information on the File memory di...

Page 182: ...t the Sonde menu selection and press Enter to display the Main sonde menu Highlight the File selection and press Enter NOTE The upload MUST be made from the File menu of the sonde not the File menu of the 650 Select the Upload or Quick upload last file and press Enter Then follow the instructions including selection of file format YSI recommends the PC6000 format Press Enter and the display will i...

Page 183: ...instrument so that readings in the NMEA 0183 protocol are generated Purchase from your GPS manufacturer a cable that connects at one end to the GPS unit and has at its other end a female DB 9 connector for interface with the YSI 6115 GPS cable Once these requirements are met proceed according to the following instructions consulting Figure 2 in Section 3 2 3 above for assistance Connect the 6115 G...

Page 184: ...emain as part of the sensor data file that can be viewed from the 650 display and or uploaded with the data file for analysis in EcoWatch for Windows Latitude and longitude are however displayed somewhat differently by the 650 and EcoWatch as noted below For both display formats the GPS are indexed relative to the point where the Greenwich Mean Time line crosses the equator For the 650 Status Bar ...

Page 185: ...ld provide readings that are useful for dissolved oxygen calibration for many months with no user attention However if the user wishes to either increase the accuracy of the sensor in the local barometric pressure range or to compensate for the small drift associated with any sensor the 650 provides a single point calibration routine which is accessed in the 650 System setup menu and is described ...

Page 186: ...h no sonde attached If your want to log barometer readings to 650 memory you must make certain that the Store Barometer option is active in the Logging setup menu of the 650 Once these conditions are met follow the instructions in Section 3 5 3 above for logging of sonde sensor readings to 650 memory The barometer readings will automatically be added to the file at the sample interval selected for...

Page 187: ...end of the YSI 655174 PC Interface cable to the 650 and the DB 9 end of the 655174 to a serial port of a PC which has Internet access as shown below Once the setup is complete access the YSI Web Page and locate the section on software upgrades Select the 650 upgrade entry and follow the instructions provided on the Web Page If you encounter difficulties in the upgrade procedure contact YSI Technic...

Page 188: ...erly to both 650 and sonde 2 Make certain that you are accessing the upload routine from the sonde File menu and NOT from the 650 File menu Upload of files from 650 to PC fails 1 Make sure that cable is connected properly to both 650 and PC 2 Make certain that the proper Comm port is selected in EcoWatch for Windows GPS and or barometer data is not stored with sonde data file Make sure Store barom...

Page 189: ...cified in EN55011 CISPR11 for Industrial Scientific and Medical laboratory equipment These ferrite assemblies are supplied as part of cable kits To install the beads follow these steps Make a small loop approximately 5 cm in diameter in the cable near the 650 MS 8 connector Lay the open ferrite bead assembly under the loop with the cable cross over position within the cylinder of the ferrite bead ...

Page 190: ...es will result in the voiding of the warranty and the compromising of the system performance but more importantly can cause safety hazards which result from overcharging such as overheating venting of gas and loss of corrosive electrolyte 4 Do not charge the battery pack outside the 0 40 C temperature range 5 Do not use or store the battery at high temperature such as in strong direct sunlight in ...

Page 191: ...use damage to skin or other tissues If any fluid from the battery pack comes in contact with a user s eyes immediately flush with clean water and consult a physician immediately The alkaline solution can damage eyes and lead to permanent loss of eyesight Warning Notifications Misuse creates a possibility of death or serious injury 1 Do not allow the battery pack to contact freshwater seawater or o...

Page 192: ...ble charger other than to change the fuse as instructed Replace the part or send it to YSI Product Service if repair is required Incorrect reassembly may result in a risk of electric shock or fire 11 To reduce risk of electric shock unplug charger before attempting any maintenance or cleaning Turning off controls will not reduce this risk 650 Water Leakage Safety Information The 650 has been teste...

Page 193: ...Range 20 to 70 Degrees Celsius Dimensions 9 13 inches long by 4 75 inches wide by 2 25 inches deep Weight with 4 Alkaline C cells and no attached sonde or cable Approximately 2 1 lbs 960 g Display VGA LCD with 320 by 240 pixels with backlight Connector MS 8 Meets IP 67 specification Standard Battery Configuration 4 Alkaline C Cells with detached battery cover Optional Battery Configuration Nickel ...

Page 194: ...n temperature Communication Protocol RS 232 to all sondes and for data transfer to PC and for software updates GPS via Y cable NMEA 0183 Standard Backlight Feature 4 LEDs illuminating LCD Keyboard 20 keys including Meter On Off Backlight On Off Enter Escape 10 Number Letter Entry Keys 2 Vertical arrow keys 2 Horizontal arrow keys 1 minus hyphen entry key 1 decimal point period ...

Page 195: ...650 MDS Section 3 YSI Environmental Monitoring Systems Operations Manual 3 65 ...

Page 196: ...650 MDS Section 3 YSI Environmental Monitoring Systems Operations Manual 3 66 ...

Page 197: ...d in EcoWatch Term Definition 1 Data display window The window that appears when you open a DAT file 2 Parameter A measurement such as temperature dissolved oxygen pH etc On a graph the data of a parameter is displayed as a trace 3 Trace The plot of the data of any one parameter There may be one or two traces per graph 4 Graph There may be one or more graphs depending upon how many parameters are ...

Page 198: ...eract with the sonde or display logger The terminal window is a communication interface with another piece of equipment whereas the Data Display Window is direct interaction with the EcoWatch software 8 Dialog box A dialog box opens to display choices Such as File Open 9 PC6000 data file A data file that is in a format compatible with EcoWatch for Windows or PC6000 software 9 Study A single data f...

Page 199: ...measurements for this point in time are displayed to the left of the graph While holding down the right mouse button move to another area on the graph Notice how the measurements change as you move When you release the mouse button the display returns to normal To view statistical information for the study click the Statistics button on the toolbar On the statistics window click on any min or max ...

Page 200: ...ata in Comma Delimited CDF format Copy the whole graph page or data from the selection on the table to the clipboard Print the active graph page or table page depending on which one is currently active Open a new terminal window to communicate with the sonde Access context sensitive help Shift F1 Toggle table window during file processing Toggle graph window during file processing Display study st...

Page 201: ...of sensors See Section 2 5 Sonde Software Setup for sonde setup 2 Deploy the Sonde For more details see Section 2 Sondes and Section 3 Displays Loggers 3 Retrieve the Sonde and Upload the Data After retrieving the sonde connect it to your PC and upload the data As with calibration and setup you can use the Sonde button in EcoWatch to communicate with the sonde Using the sonde menu upload the data ...

Page 202: ...for Windows Section 4 YSI Incorporated Environmental Monitoring Systems Manual 4 6 5 Print Your Graph To print the graph open the File menu choose Print Setup or Print to choose exactly how the print should look ...

Page 203: ...0 DISPLAY LOGGER EcoWatch may be used with various sondes or display loggers To utilize the configuration that will work best for your application make sure that you have all of the components that are necessary You will need 650 YSI 655174 PC interface cable PC with active serial port 650 PC Serial port DE 9 655174 PC Interface Cable 650 MDS to Lab Computer ...

Page 204: ...ILE FROM A SONDE Data that is processed in EcoWatch typically originates in a sonde with batteries and is uploaded to a PC To upload data from a YSI sonde connect the sonde to your PC and open the EcoWatch software Use the Sonde button on the toolbar to communicate with the sonde software Using the sonde software go to the Main menu by typing menu on the blank terminal screen Then choose 3 File Me...

Page 205: ...ure YSI upgrades After transfer the file will be located in the C ECOWWIN DATA subdirectory of your PC hard drive 4 2 4 USING THE GRAPH Once uploaded data can be easily displayed in EcoWatch Open a DAT file The six buttons on right side of the toolbar help you get a closer view of that portion of the graph you desire Zoom Window Use this to select a certain portion of the graph to be displayed Cli...

Page 206: ...ew the study from the beginning limit to the end limit This command can be used on both graphs and data tables Zoom In Click this button to magnify by 20 This command can be used on both graphs and data tables Zoom Out Click this button to reduce magnification by 20 This command can be used on both graphs and data tables ...

Page 207: ...ments If you do have a default port selected then the program will use that port automatically The number of parameters displayed during real time is set by the sonde If four parameters are set in the Report setup in the sonde then those same parameters will be displayed in real time If you want to change the parameters displayed then you must close the real time window open a sonde terminal windo...

Page 208: ...ons you select in the List Files of Type box List of Files of Type Select the type of file that you want to open dat Sonde binary data file txt prn ASCII text file cdf csv sdf Comma delimited file rt Real time file cr1 CR10 file Drives Select the drive where the PC6000 data file resides Directories Select the directory where the PC6000 data file resides Network Choose this button to connect to a n...

Page 209: ... document s window as shown below IMPORT With this command you can import common ASCII files into EcoWatch The file will be converted into PC6000 format and again with a DAT extension MERGE This function takes two existing data files and merges them into one new file Both files must be in PC6000 format DAT have the same number of parameters and the same parameter types the parameter setup must be ...

Page 210: ...anual 4 14 COPY TO CLIPBOARD If a graph or table is active then choosing this command will send its contents to the Windows clipboard so that it can be pasted into other programs This is a normal method of transfer data between different Windows programs Shortcuts Toolbar Keys CTRL C ...

Page 211: ...time and have it look exactly the same as when you saved it This saved display is associated with the data file Up to 9 data displays can be saved per data file When you save a display you will be asked to give it a name in the following dialog box There must be an open data file for this command to be available See also Load Data Display Shortcuts Keys CTRL S LOAD DATA DISPLAY This command is onl...

Page 212: ...rms a WMF file or a CDF file WMF stands for Windows MetaFile and is a format that describes any image in a way that is independent of the program that generated the image For example you can generate a graph in EcoWatch export it as a WMF file and then import it into another Windows program CDF stands for Comma Delimited sometimes referred to as a Comma and Quote Delimited File This format is comm...

Page 213: ...owse button if needed If you want to simply have a report Type listing the data then click Data If you want Statistics minimum maximum average and standard deviation then the statistics frequency button applies If you want to have Daily statistics keep in mind that every day actually starts at midnight It would be likely then that the first and last days would have very different statistics becaus...

Page 214: ...then the program will size the graph so that it fits on one page If you choose Exact size then program will print the graph as close as possible to the size that is displayed Maintain Aspect Ratio If you choose to maintain the aspect ratio then the program will size the graph until either the height or the width is maximized and then leave the graph in the same height width ratio that is displayed...

Page 215: ...ommand presents the Print Setup dialog box The following options allow you to select the destination printer and its connection Printer Select the printer you want to use Choose the Default Printer or choose the Specific Printer option and select one of the current installed printers shown in the box You install printers and configure ports using the Windows Control Panel ...

Page 216: ...al choices about printing specific to the type of printer you have selected Network Displays a dialog box that allows you to choose a printer if you are on a network PAGE SETUP Layout options for printable data tables may be specified Page margins table setup page orientation and page order are adjustable HEADER FOOTER Page headers and footers can be customized with respect to font and placement B...

Page 217: ...s command will send its contents to the Windows clipboard so that it can be pasted into other programs This is a normal method of transfer data between different Window programs Copying data to the clipboard replaces the contents previously stored there Shortcuts Toolbar Keys CTRL C PASTE This command does not function in this version of EcoWatch REMOVE PARAMETERS When entire column s of data is s...

Page 218: ... the following commands TOOLBAR Use this command to display and hide the Toolbar which includes buttons for some of the most common commands in EcoWatch such as File Open A check mark appears next to the menu item when the Toolbar is displayed Shortcut To hide or display the Toolbar choose Toolbar from the View menu See Section 4 2 6 Using the Toolbar for a list of the toolbar icons and their func...

Page 219: ...ts Toolbar TABLE Use this command to display and hide the Table page while viewing a data file Shortcuts Toolbar GRID Turns on or off the gridlines on graphs MARKERS Turns on or off data point markers on the graphs For graphs with more than a few data points we recommend that Markers be left off STATISTIC This command will display statistics for the current study It will show the minimum maximum m...

Page 220: ...l 4 24 Shortcuts Toolbar STUDY INFO This command will display a dialog box describing the study It will show the sonde type and serial number that was used to collect the data the parameters available the logging interval and the beginning and ending times of the sample Shortcuts Toolbar ...

Page 221: ...be dimmed Shortcuts Toolbar UNZOOM Displays the graph all the way to both ends of the Study Limit if a graph is selected Displays the data table in default size if a data table is selected Shortcuts Toolbar 4 3 4 COMM UNICATION The Comm Communications menu commands let you control your communications settings with sondes modems and other devices Several communication menu options are only availabl...

Page 222: ...ected to the port You may choose independent settings for each communication port Protocol You may choose Kermit Xmodem or Xmodem 1K protocol for file transfer through the communications port 6820 6920 600R 600XL 600XLM and 6600 sondes use only Kermit protocol Handshaking You may choose Xon Xoff or RTS CTS or Both or None Xon Xoff is often referred to as software handshaking and RTS CTS as hardwar...

Page 223: ...he Custom modem If your modem does not work select Hayes Compatible modem Those settings are quite common and might work for your modem If neither approach works then you will have to define your own settings You will need the instruction manual for your modem It should list all of the proper settings Choose Custom from the Modem list and enter the settings for your modem The Auto Answer setting i...

Page 224: ...onde and default capture files for each sonde port This tab addresses several COM port settings not covered on the other two tabs Default Capture File While communicating to a sonde in terminal mode using the Sonde command you may want to capture measurements to a file You may type in a default file name in the accompanying text box If you specify a file name here and select the Use on Capture che...

Page 225: ...port in the Communications Settings dialog box the following dialog box will appear asking you which COM port to use Shortcuts Toolbar TERMINAL Opens a new terminal window for communicating with any compatible RS 232 devices FONT COLOR Adjust font color size and style for any text that appears in the terminal window BACKGROUND COLOR Adjust the background color of the terminal window CAPTURE Turns ...

Page 226: ...he upload from the device then choose this command This command is enabled only when the Xmodem or Xmodem 1K protocol has been selected in Port Setup DIAL MODEM If you have specified a default phone number in the Modem tab of the communications Settings dialog box then this command will call that number If you have not specified a default number then you will be asked for a number HANG UP MODEM Ha...

Page 227: ... then you may want to clean the optics periodically Set that interval in this dialog Autoscaling will keep the trace comfortably on the graph as the measurement changes To Manually Scale a graph double click on its Y axis labels The Graph Y Axis box will come up where you can set the scale Note that you can manually set the scale for some parameters and autoscale others When the autoscale box is c...

Page 228: ...sted in The program will have fewer data points to handle and will respond to your commands quicker Executing this command when modifying a graph will change the pointer to a vertical arrow Click once on the left end of that part of the graph that you want and then click again at the right end This command stays active until you execute it again To limit the data in a table move the pointer to the...

Page 229: ...e others When the autoscale box is checked the high and low limits will be dimmed See also the Autoscale command in the Graph menu REDRAW If part of a graph is not displayed it could be because your window is sized too small to display it or because you have just returned to EcoWatch from some other application Maximizing the window and choosing this command should display your graph correctly Sho...

Page 230: ...the Selected Parameters list in the order that they were added To rearrange the order highlight the parameter you wish to move and then use the up and down buttons to move it to the desired spot in the order The TSS parameter only available if your sonde has a Turbidity sensor is a parameter that is not part of your original collected data TSS is calculated by EcoWatch which uses the Turbidity dat...

Page 231: ...nterval to 60 minutes each set of four points will be averaged and then plotted as a single point This feature will not only smooth a graph but will also reduce the amount of data exported that is sometimes convenient when exporting to a spreadsheet The x axis parameter is normally Time but you can choose any parameter you like By default data files are sorted by increasing time If you have chosen...

Page 232: ...ters This command opens the TSS Calibration window and allows calibration adjustment How do I add TSS to my data file You must have a data set that includes Turbidity data points It is also common to use a 0 to 0 correlation as a base point in these measurements This can be used as the first correlation point You need to establish a correlation between your Turbidity reading and a TSS value This i...

Page 233: ...have entered the desired number of correlation points click OK EcoWatch will calculate the TSS values by doing a linear interpolation of the Turbidity NTU data and the correlation chart GRAPH The Setup menu Graph submenu has the following commands Font Color Goes to the Font Dialog Box where you can change the font and color of text that appears in the graph Font Type or select a font name EcoWatc...

Page 234: ...ting you specify before you apply it to the document Page Color Goes to the Color Dialog Box where you can change the color of the graph page This is the background for all of the graphs rather than the background for each individual graph that is set with the next command Trace Color Goes to the Change Trace Color dialog box where you can change the color of the trace and the background for each ...

Page 235: ...or Dialog Box where you can change the color of the table background color That is the part of the table where the readings appear Highlight Color Goes to the Color Dialog Box where you can change the color of the table highlight color That is the part of the table where the reading units appear SET DEFAULT TEMPLATE This option allows you to select display parameters for your data when you initial...

Page 236: ...e the display parameters that you have chosen If you want to delete your template enter the Setup Menu and choose Clear Default Template CLEAR DEFAULT TEMPLATE This command will eliminate a default template that has been saved With no default template all new EcoWatch files open in whatever form they were saved in 4 3 8 APPL ICATIONS The Appl menu is the link to other Application programs that can...

Page 237: ...ped horizontal tiles TILE VERTICAL Use this command to arrange multiple opened windows side by side ARRANGE ICONS Use this command to arrange the icons for minimized windows at the bottom of the main EcoWatch window If there is an open document window at the bottom of the main window then some or all of the icons may not be visible because they will be underneath this document window WINDOW 1 2 Ec...

Page 238: ...lp menu offers the following commands which provide you assistance with this application CONTENTS Offers you a list of the available Help topics USING HELP Use this command for instructions about using Help TECHNICAL SUPPORT YSI Technical support phone number ABOUT Displays the version number of this application ...

Page 239: ...ly not known it is best to report a conductivity at a particular temperature e g 20 2 mS cm at 14 o C However in many cases it is also useful to compensate for the temperature dependence in order to determine at a glance if gross changes are occurring in the ionic content of the medium over time For this reason the sonde software also allows the user to output conductivity data in either raw or te...

Page 240: ...and was reported Hence the designation ppt is reported by the instrument to provide a more conventional output 5 3 TOTAL DISSOLVED SOLIDS TDS The electrical conductivity of environmental water is due to the presence of dissolved ionic species Thus the magnitude of the conductivity or specific conductance value can be used as a rough estimate of amount in g L of these ionic compounds which are pres...

Page 241: ...versus the Ag AgCl reference electrode of the combination sensor that utilizes gelled electrolyte ORP values are presented in millivolts and are not compensated for temperature CALIBRATION AND EFFECT OF TEMPERATURE Calibration may not be required for the ORP sensor of the sonde when it is new However older probes that have been deployed extensively may show some deviation from the theoretical ORP ...

Page 242: ... Since the hydrogen ion concentration in the internal buffer solution is invariant this potential difference determined relative to the Ag AgCl reference electrode is proportional to the pH of the media The pH system of the 600R utilizes individual glass sensing and reference electrodes but the operating principle is the same YSI offers three styles of pH and pH ORP relative to the glass portion o...

Page 243: ...K 25 C then the slope of the plot at 313 K 40 C must be 313 298 59 62 mv pH unit At 283 K 10 C the slope is calculated to be 56 mv pH unit 283 298 59 Determination of the slope of pH vs mv plots at temperatures different from Tc is thus relatively simple In order to establish the intercept of the new plot the point where plots of pH vs mv at different temperatures intersect the isopotential point ...

Page 244: ...ting for atmospheric pressure introduces a small error Because the software uses the atmospheric pressure at the time of calibration changes in atmospheric pressure between calibrations appear as changes in depth The error is equal to 0 045 feet for every 1mm Hg change in atmospheric pressure In sampling applications frequent calibrations eliminate the error Considering typical changes in baromete...

Page 245: ...n consume much of the battery reserve in a portable system or by manually agitating the sonde when carrying out spot sampling applications which can be inconvenient These disadvantages are overcome by the Rapid Pulse dissolved oxygen technology that is associated with the sonde because it needs no stirring to yield accurate readings In addition because of the nature of the technology some effects ...

Page 246: ...ystem to be calibrated with a single medium air or water of known oxygen pressure CALIBRATION AND EFFECT OF TEMPERATURE The sonde Rapid Pulse system is calibrated using the same basic methods employed for steady state oxygen sensors However the software that controls the calibration protocol is somewhat different depending on whether the unit will be used in sampling or deployment studies For samp...

Page 247: ...rcent saturation reading is empirically derived while the conversion from percent saturation and temperature to solubility in mg L is carried out automatically by the sonde software using formulae available in Standard Methods for the Examination of Water and Wastewater ed 1989 See Appendix D Solubility and Pressure Altitude Tables for dissolved oxygen solubility tables as a function of salinity a...

Page 248: ...ional to the amount of oxygen present and the relationship between the oxygen pressure outside the sensor and the lifetime can be quantified by the Stern Volmer equation For most lifetime based optical DO sensors including the YSI 6150 this Stern Volmer relationship Tzero T 1 versus O2 pressure is not strictly linear particularly at higher oxygen pressures and the data must be processed using anal...

Page 249: ...ntains 9 09 mg L at 20 o C but only 7 65 mg L at 30 o C The sonde compensates for both of these temperature related factors after instrument calibration The temperature compensation for the percent saturation reading is empirically derived while the conversion from percent saturation and temperature to solubility in mg L is automatically carried out using formulae available in Standard Methods for...

Page 250: ...R IN THE VESSEL DOES NOT COMPLETELY EVAPORATE DURING THE REHYDRATION STEP 6 To assure that the best possible accuracy for the ROX Optical DO sensor YSI recommends that the membrane assembly be replaced after 1 year The 6155 Optical DO Membrane Replacement Kit can be ordered from YSI Technical Support For more information on the practical aspects of using the 6150 ROX dissolved oxygen sensor see Ap...

Page 251: ...ously low because of interference Unusually high nitrate readings which could be due to interfering ions should be confirmed by laboratory analysis after collection of water samples Ion selective electrodes have the greatest tendency to exhibit calibration drift over time of all the sensors available on the sonde This drift should not be a major problem for sampling studies where the instrument ca...

Page 252: ...hat depends on the relative amounts of ammonium in the sample and the internal filling solution This potential is read relative to the reference electrode of the sonde pH probe As for all ISEs there is a linear relationship between the logarithm of the ammonium activity or concentration in dilute solution and the observed voltage The Nernst equation describes this relationship Under ideal conditio...

Page 253: ...equently calibrated However if an ammonium sensor is used in a longer term deployment study with the sonde the user should be aware that drift is almost certain to occur The extent of the drift will vary depending on the age of the probe the flow rate at the site and the quality of the water For all monitoring studies using ion selective electrodes the user should acquire a few grab samples during...

Page 254: ... 59 mV for every 10 fold rise in chloride activity at 25 C However in practice empirical calibration of the electrode is necessary to establish the slope of the response Typical slopes are 45 55 mV per decade for YSI sensors This slope value is determined by calibration with two solutions of known chloride concentration typically 10 mg L and 1000 mg L Cl The slope of the plot of log chloride vs vo...

Page 255: ...will provide a default setting for the effect of temperature on your particular sensor After this initial calibration you can use the less time consuming 2 point and 1 point routines to update the 3 point calibration However we strongly recommend a new 3 point calibration after each deployment of 30 days or longer 2 Ion selective electrodes may not stabilize as rapidly as pH sensors Be sure to all...

Page 256: ...cally be reported in the units turbid NTU The Model 6136 has been the only turbidity probe sold by YSI since 2002 and it is characterized by relatively large optics compared to the 6026 Since these larger optics result in deeper penetration of the light beam into the sample the use of a longer probe guard is required a slight disadvantage However the larger optical cell volume of the sensor has tw...

Page 257: ...ions or will function automatically during long term unattended sampling studies The number of wiper movements and the frequency of the cleaning cycle for the unattended mode can be set in the sonde software Generally one movement is sufficient for most environmental applications but in media with particularly heavy fouling additional cleaning cycles may be necessary CALIBRATION AND EFFECT OF TEMP...

Page 258: ...vating the wiper of the 6026 or 6136 from a computer or 650 MDS keypad removes these bubbles 3 When calibrating the 6136 sensor be aware of the fact that precautions must be taken to avoid interference of the bottom of the calibration vessel Instructions for two methods of calibrating the 6136 sensor are provided in Section 2 6 1 of this manual Unless these precautions are taken field turbidity re...

Page 259: ...action of the chlorophyll from the disrupted cells into the organic solvent acetone The extract is then analyzed by either a spectrophotometric method using the known optical properties of chlorophyll or by high performance liquid chromatography HPLC This general method is detailed in Section 10200 H of Standard Method and has been shown to be accurate in multiple tests and applications as long as...

Page 260: ...he 470 nm exciting light from being detected when it is backscattered off of particles in the water Without the filter turbid cloudy water would appear to contain fluorescent phytoplankton even though none were present The following diagram can be used to better understand the principles of the YSI system Most commercial fluorometers fit into two categories In the first category are benchtop instr...

Page 261: ...ly comprises the following steps 1 Prior to use in the field place the sensor in clean water and perform a 1 point calibration at 0 ug L 2 Immerse the sonde in a dye standard see below and record the reading Note that you are not calibrating to the dye reading only checking its value relative to the default sensitivity of the sensor 3 While making your field readings sampling or unattended studies...

Page 262: ...Transfer this standard to a glass bottle and retain it for future use 3 Accurately transfer 5 0 mL of the solution prepared in the above step to a 1000 mL volumetric flask and then fill the flask to the volumetric mark with deionized or distilled water Mix well to obtain a solution which is 0 5 mg L in water a 200 1dilution of the concentrated solution 4 Store the concentrated standard solution pr...

Page 263: ...menu From our studies it appears that entry of a value of 1 to 2 per degree C for Chl tempco is appropriate to partially account for changes in the fluorescence of environmental phytoplankton with temperature This value can be estimated in the above example as follows Change in Temperature 21 1 20 C Change in Fluorescence 226 185 41 µg L Change in Fluorescence 41 185 x 100 22 1 Chl Tempco Factor 2...

Page 264: ...rophyll measurement INTERFERENCES FROM OTHER FLUORESCENT SPECIES The analytical methods described in Standard Methods for chlorophyll involve disruption of the living organisms present in suspension followed by extraction of molecular chlorophyll into a homogeneous solution in an organic solvent Acidification of the extract helps to minimize the interferences caused by a number of other non chloro...

Page 265: ...on suspension at cold temperature would erroneously indicate the presence of more phytoplankton than when the suspension is read at room temperature Unless this effect is taken into account most field readings will be somewhat in error since the field temperature will differ from the temperature of calibration The use of the Chl Tempco factor found in the Advanced Sensor menu will help to reduce t...

Page 266: ... the YSI 6130 Rhodamine WT sensor works on the same principles as described above for the 6025 chlorophyll sensor As for chlorophyll Rhodamine WT fluoresces that is when irradiated with light of a particular wavelength it emits light of a higher wavelength or lower energy The ability of Rhodamine WT to fluoresce is the basis for all commercial fluorometers capable of measuring the analyte in situ ...

Page 267: ...the frequency of the cleaning cycle for the unattended mode can be set in the sonde software Generally one movement is sufficient for most environmental application but in media with particularly heavy fouling additional cleaning cycles may be necessary CALIBRATION AND EFFECT OF TEMPERATURE The sonde software offers the option of 1 point 2 point or 3 point calibrations procedures For most applicat...

Page 268: ...n Rhodamine WT standards are required in the future perform another dilution of the concentrated dye solution after warming it to ambient temperature Our experience has indicated that the concentrated solution that has been kept at cold temperatures is much more stable than the dilute solution stored at room temperature EFFECT OF TURBIDITY ON RHODAMINE WT READINGS As described above the filters in...

Page 269: ...stem for potentially hazardous conditions In addition to potential toxin production BGA blooms can also result in water with an unpleasant appearance and in the case of drinking water an unpleasant taste and odor These problems adversely affect water quality and diminish the water s recreational utility Also of concern are high cell concentrations causing an increase in filter run times in drinkin...

Page 270: ...BGA cells is the basis for all commercial fluorometers capable of measuring the analyte in vivo These instruments induce phycocyanin to fluoresce by shining a beam of light of the proper wavelength into the sample and then measuring the longer wavelength light which is emitted as a result of the fluorescence process The YSI 6131 Phycocyanin Probe uses an orange light emitting diode LED for the exc...

Page 271: ... produce a ratio for adjustment or postcalibration of your field results into more accurate values 7 Use the calculating power of the spreadsheet to multiply all of your field readings by the correction ratio to obtain the best possible accuracy for your 6131 data A variation of this method is to perform a 2 point calibration prior to deployment using a clear water and a dye standard setting the a...

Page 272: ...factor in the Advanced Sensor menu MUST BE SET TO ZERO when calibrating with Rhodamine WT Table Approximate PC containing equivalent of 100 ug L Rhodamine WT as a function of temperature T C Cells mL to Enter T C Cells mL to Enter 30 44940 18 76580 28 49700 16 83580 26 54600 14 91420 24 58940 12 98140 22 64120 10 107940 20 70000 8 113540 REMEMBER The use of Rhodamine WT for calibration of the Phyc...

Page 273: ...emperature compensation routine PC tempco resident in the sonde firmware under the Advanced Sensor menu where the factor in per degree C can be input by the user The value of this factor can be estimated as follows using a single suspension of PC containing BGA under laboratory conditions In the experiment the cells mL value of the suspension is measured at both ambient temperature and then at a m...

Page 274: ...rregular clumps or pinhead sized spheres When BGA colonize into these forms the sensitivity of the YSI sensor in terms of the fluorescence per cell of BGA is reduced because it has been designed to detect microscopic free floating cells and not large macroscopic floating particles Thus the sensor is likely to underestimate the total amount of BGA present in the water when clumps are present Users ...

Page 275: ...in the known volume of sample water While accurate these types of analytical techniques usually are done as part of a spot sampling protocol and almost never yield continuous data with regard to BGA content The methods are time consuming and usually require an experienced efficient analyst to generate consistently accurate and reproducible results Most importantly the methods do not lend themselve...

Page 276: ... photodiode of high sensitivity that is screened by an optical filter that restricts the detected light The filter prevents the green exciting light from being detected when it is backscattered off of particles in the water Without the filter turbid cloudy water would appear to contain significant amounts of PE containing BGA even though none were present The following diagram can be used to bette...

Page 277: ...ults to produce a ratio for adjustment or postcalibration of your field results into more accurate values 14 Use the calculating power of the spreadsheet to multiply all of your field readings by the correction ratio to obtain the best possible accuracy for your 6132 data A variation of this method is to perform a 2 point calibration prior to deployment using a clear water and a dye standard setti...

Page 278: ...nsor menu MUST BE SET TO ZERO when calibrating with Rhodamine WT Table Approximate PE containing equivalent of 8 ug L Rhodamine WT as a function of temperature T C Cells mL to Enter T C Cells mL to Enter 30 156 000 18 210 000 28 164 000 16 220 000 26 174 000 14 230 000 24 181 000 12 240 000 22 189 000 10 247 000 20 200 000 8 254 000 REMEMBER The use of Rhodamine WT for calibration of the PE Probe ...

Page 279: ... firmware under the Advanced Sensor menu where the factor in per degree C can be input by the user The value of this factor can be estimated as follows using a single suspension of PE containing BGA under laboratory conditions In the experiment the cells mL value of the suspension is measured at both ambient temperature and then at a much colder temperature by cooling the suspension in a refrigera...

Page 280: ...ps or pinhead sized spheres When BGA colonize into these forms the sensitivity of the YSI sensor in terms of the fluorescence per cell of BGA is reduced because it has been designed to detect microscopic free floating cells and not large macroscopic floating particles Thus the sensor is likely to underestimate the total amount of BGA present in the water when clumps are present Users should take c...

Page 281: ... application The weir or flume is referred to as the primary measuring device and the level meter is referred to as the secondary measurement device There are commonly 3 types of weirs and 7 types of flumes Most of these are available in a number of sizes Flow level curves for common types and sizes are already programmed in the sonde so that it is only necessary to describe the primary measuring ...

Page 282: ...low based on an equation The equation must be of the form This allows the use of primary measuring devices other than the standard ones already programmed Users may enter values for K1 P1 K2 and P2 and the sonde will calculate flow Note if you do not need the second term in the equation simply enter 0 for K2 TABLE YSI sondes that are equipped with shallow vented level can also calculate flow based...

Page 283: ...ance and Storage After resurfacing the probe activate the DO charge parameter in the Report setup section of the sonde software and confirm that the value is within the acceptable range of 50 25 After resurfacing allow the sensor to pulse in the Run mode for at least 5 minutes during which time the DO charge may be expected to drop in value if the sensor is still functional If resurfacing accordin...

Page 284: ...connectors 2 3 4 Damaged connectors Check pins at both ends insure they are straight dry and clean Com port not selected Change to other com port other peripheral on the same port Internal mouse Try other PC 650 display logger or dumb terminal 2 4 3 Scrambled data Unmatched baud rate between host and sonde Match the baud rate 2 4 3 Host is too slow Use faster computer Interface cable failure Check...

Page 285: ...trolyte Replace electrolyte and membrane 2 3 1 DO probe has been damaged Replace 6562 probe 9 Internal failure Return sonde for service 9 ROX dissolved Oxygen reading unstable or inaccurate Probe not properly calibrated Follow DO cal procedures 2 6 1 Membrane assembly not properly installed or may be punctured Follow instructions for proper membrane assembly replacement 2 10 2 Water in probe conne...

Page 286: ... Use new calibration solution C Internal failure Return sonde for service 8 Calibration solution or sample does not cover entire sensor Immerse sensor fully 2 6 1 Installed probe has no reading The sensor has been disabled Enable sensor 2 9 7 Water in probe connector Dry connector reinstall probe 2 3 2 Probe has been damaged Replace the 6560 probe 9 Report output improperly set up Set up report ou...

Page 287: ... further detail into the sondes RS 232 and SDI 12 implementations see Sections 7 3 and 7 4 respectively With these configurations the Sonde is capable of interfacing to a variety of devices from a dumb terminal to numerous data collection platforms 7 2 HARDWARE INTERFACE Connection from the Sonde to the host computer is provided using the YSI 6095B MS 8 to DB 9 female adapter This 6095B then conne...

Page 288: ... as 10 sensors can be connected to the bus at a time Each sensor is pre assigned a unique address from 0 to 9 Each Sonde is factory set to address 0 The address can be changed in the System menu see Section 2 9 5 System menu for details Running the sonde in SDI 12 mode requires it to be connected to a SDI 12 master device An example of such a device is the YSI 6200 or units from Campbell Scientifi...

Page 289: ...olds the sonde s software version number 100 for version 1 00 Master aD0 to aD9 Retrieve measurement verifying data Sonde a values CR LF values 33 characters or less This field holds one or more values resulting from a measurement or verifying sequence A value contains between 1 to 7 digits with an optional radix mark period or comma Each value must be preceded by its sign either or since the sign...

Page 290: ...available Sonde 1 CR LF Master 1D0 Sonde 1 17 5 12 05 98 7 8 25 6 45 CR LF Master 1D1 Sonde 1 325 10 CR LF After finishing the measurement the Sonde sent a service request to indicate completion The bus master then sent the D0 command to retrieve the data There were 5 data returned Since 7 readings should be available the master continued with D1 command and received the remaining data The respons...

Page 291: ...login password via return E mail Click on the Software folder under the Software Downloads section Inside the folder click on the file 6 Series 556MPS Code Updater M DD YYYY and save the file to a temporary directory on your computer After the download is complete run the file that you just downloaded and follow the on screen instructions to install the YSI Code Updater on your computer If you enc...

Page 292: ...ncorporated Environmental Monitoring Systems Operations Manual 8 2 When the update is finished indicated on the PC screen as shown below close the YSI Code Updater window on the PC by clicking on the X in the upper right corner of the window ...

Page 293: ...y equipment that has been damaged by their batteries it is essential that leaky or defective batteries be retained with the damaged product until the manufacturer has evaluated the claim Please contact the YSI Technical Support Group if you have any questions Damage due to accidents misuse tampering or failure to perform prescribed maintenance is not covered The warranty period for chemicals and r...

Page 294: ...esented for service without a Cleaning Certificate and if in our opinion it represents a potential biological or radioactive hazard our service personnel reserve the right to withhold service until appropriate cleaning decontamination and certification has been completed We will contact the sender for instructions as to the disposition of the equipment Disposition costs will be the responsibility ...

Page 295: ...aminate items to insure the safety of the handler Complete and include the Product Return Form which is on the next page of this section Place the product in a plastic bag to keep out dirt and packing material Use a large carton preferably the original and surround the product completely with packing material Insure for the replacement value of the product ...

Page 296: ...Warranty and Service Information Section 9 YSI Incorporated Environmental Monitoring Systems Operations Manual 9 4 9 5 PRODUCT RETURN FORM ...

Page 297: ...Warranty and Service Information Section 9 YSI Incorporated Environmental Monitoring Systems Operations Manual 9 5 ...

Page 298: ...ndations Provide adequate ventilation Avoid conditions that cause misting or splashing Remove to fresh air Give artificial respiration and get medical attention as needed May cause irritation with repeated exposure Skin Wear water resistant gloves as needed Wash exposed areas with soap and water for 15 minutes Remove contaminated clothing and wash before re using Can cause irritation and potential...

Page 299: ...act may cause irritation prolonged or repeated exposure may cause Dermatitis Eye contact may cause irritation or conjunctivitis Ingestion may cause nausea vomiting and diarrhea FIRST AID INHALATION Remove victim from exposure area Keep victim warm and at rest In severe cases seek medical attention SKIN CONTACT Remove contaminated clothing immediately Wash affected area thoroughly with large amount...

Page 300: ...astrointestinal irritation and circulatory disturbances FIRST AID INHALATION Remove victim from exposure area to fresh air immediately If breathing has stopped give artificial respiration Keep victim warm and at rest Seek medical attention immediately SKIN CONTACT Remove contaminated clothing immediately Wash affected area with soap or mild detergent and large amounts of water approx 15 20 minutes...

Page 301: ...ul quantities form the solutions is considered unlikely given the low concentration of lithium and the volumes likely to be handled FIRST AID INHALATION Remove victim from exposure area to fresh air immediately If breathing has stopped give artificial respiration Keep victim warm and at rest Seek medical attention immediately SKIN CONTACT Remove contaminated clothing immediately Wash affected area...

Page 302: ...ly with large amounts of water approx 15 20 minutes occasionally lifting upper and lower lids Seek medical attention immediately INGESTION Immediately rinse out mouth with large quantities of water If reagent was swallowed give 2 glasses of water and seek medical attention immediately YSI Nitrate Standard Solutions 3885 3886 and 3887 FIRST AID INHALATION Remove to fresh air If not breathing give a...

Page 303: ...AUTION AVOID INHALATION SKIN CONTACT EYE CONTACT OR INGESTION MAY AFFECT MUCOUS MEMBRANES YSI Turbidity Standard 6073 FIRST AID SKIN CONTACT Remove contaminated clothing Wash affected area with soap or mild detergent and water EYE CONTACT Wash eyes immediately with large amounts of water approx 15 20 minutes occasionally lifting upper and lower lids If irritation occurs seek medical attention imme...

Page 304: ...erference will not occur in a particular installation If this equipment does cause interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures θ Reorient the receiving antenna θ Relocate the computer with respect to the receiver θ Move the computer away f...

Page 305: ...Required Notice Appendix B YSI Incorporated Environmental Monitoring Systems Operations Manual B 2 ...

Page 306: ...6560 Conductivity Temperature Probe θ 6561 pH Probe θ 6561FG Flat Glass pH Probe θ 6562 Rapid Pulse DO Probe θ 6150 ROX Optical DO Probe θ 6565 Combination pH ORP Probe θ 6565FG Flat Glass Combination pH ORP Probe θ 6566 Combination pH ORP Probe Fouling Resistant θ 6025 Chlorophyll Probe wiped θ 6130 Rhodamine WT Probe wiped θ 6131 Phycocyanin Probe wiped θ 6132 Phycoerythrin Probe wiped θ 6136 Tu...

Page 307: ... Manual German θ 116275 Extended Length Calibration Cup Assembly for 6600V2 2 6600EDS V2 2 and 6600V2 4 Sondes θ 655488 Extended Length Calibration Cup Sleeve for 6820V2 1 6820V2 2 6920V2 1 and 6920V2 2 Sondes θ 066267 Decreased Length Calibration Cup Sleeve for 6600V2 4 Sonde FLOW CELLS FOR THE SONDES θ 6601 Flow Cell for 6600V2 2 Sonde θ 131041460 Endeco Part Flow Cell for 6600EDS V2 2 and 6600V...

Page 308: ... of pH 4 pH7 and pH 10 Buffers ORP REAGENTS θ 3682 Zobell Solution 125 ml AMMONIUM REAGENTS θ 3841 1 mg L ammonium nitrogen standard solution θ 3842 10 mg L ammonium nitrogen standard solution θ 3843 100 mg L ammonium nitrogen standard solution NITRATE REAGENTS θ 3885 1 mg L nitrate nitrogen standard solution θ 3886 10 mg L nitrate nitrogen standard solution θ 3887 100 mg L nitrate nitrogen standa...

Page 309: ...θ 5085 Hands Free Harness θ 5065 Form fitting Case θ 6930 Hard sided Case θ 6655 Soft sided Case θ 6124 Backpack θ 6117 Extra Rechargeable Battery Pack for 650 MDS θ 6115 GPS Cable for interface with user supplied GPS unit θ 616 Cigarette Lighter Charger LOGGER TELEMETRY θ 6200 Data Acquisition System 6200 DAS and Accessories Contact YSI Massachusetts 1 800 363 3269 for options and ordering inform...

Page 310: ...pt 45 2 ppt 0 0 14 62 13 73 12 89 12 10 11 36 10 66 1 0 14 22 13 36 12 55 11 78 11 07 10 39 2 0 13 83 13 00 12 22 11 48 10 79 10 14 3 0 13 46 12 66 11 91 11 20 10 53 9 90 4 0 13 11 12 34 11 61 10 92 10 27 9 66 5 0 12 77 12 02 11 32 10 66 10 03 9 44 6 0 12 45 11 73 11 05 10 40 9 80 9 23 7 0 12 14 11 44 10 78 10 16 9 58 9 02 8 0 11 84 11 17 10 53 9 93 9 36 8 83 9 0 11 56 10 91 10 29 9 71 9 16 8 64 1...

Page 311: ...1 6 18 28 0 7 83 7 44 7 08 6 73 6 40 6 09 29 0 7 69 7 32 6 96 6 62 6 30 5 99 30 0 7 56 7 19 6 85 6 51 6 20 5 90 31 0 7 43 7 07 6 73 6 41 6 10 5 81 32 0 7 31 6 96 6 62 6 31 6 01 5 72 33 0 7 18 6 84 6 52 6 21 5 91 5 63 34 0 7 07 6 73 6 42 6 11 5 82 5 55 35 0 6 95 6 62 6 31 6 02 5 73 5 46 36 0 6 84 3 52 6 22 5 93 5 65 5 38 37 0 6 73 6 42 6 12 5 84 5 56 5 31 38 0 6 62 6 32 6 03 5 75 5 48 5 23 39 0 6 5...

Page 312: ...52 699 932 2290 698 92 27 24 692 922 2587 789 91 26 93 684 912 2887 880 90 26 61 676 902 3190 972 89 26 34 669 892 3496 1066 88 26 02 661 882 3804 1160 87 25 75 654 871 4115 1254 86 25 43 646 861 4430 1350 85 25 12 638 851 4747 1447 84 24 84 631 841 5067 1544 83 24 53 623 831 5391 1643 82 24 25 616 821 5717 1743 81 23 94 608 811 6047 1843 80 23 62 600 800 6381 1945 79 23 35 593 790 6717 2047 78 23...

Page 313: ...able 4 Conversion Factors for Common Units of Pressure kilo Pascals mm Hg millibars inches H20 PSI inches Hg 1 atm 101 325 760 000 1013 25 406 795 14 6960 29 921 1 kiloPascal 1 00000 7 50062 10 0000 4 01475 0 145038 0 2953 1 mmHg 0 133322 1 00000 1 33322 0 535257 0 0193368 0 03937 1 millibar 0 100000 0 750062 1 00000 0 401475 0 0145038 0 02953 1 inch H20 0 249081 1 86826 2 49081 1 00000 0361 0 073...

Page 314: ...luting 50 mL of 100 NTU standard to a total volume of 500 mL will yield a 10 NTU standard You can also use formazin as your source for turbidity standards The formazin can be generated by the procedure found in Standard Methods for the Examination of Water and Wastewater or it can be purchased from Hach as a 4000 NTU suspension that can be linearly diluted to form lower NTU standards In addition H...

Page 315: ...c calibration also confirms that the sensor is performing properly with regard to its sensitivity and general function Our empirical testing has indicated that the optical system of the 6136 probe is very stable and likely to require only infrequent calibration However you should initially confirm the stability of the sensor for your typical sampling or monitoring application by frequently checkin...

Page 316: ...ean bubbles and fouling from the optics of the probe and minimize the chance of corrupted readings due to these factors Note however that for Unattended applications with the sonde where data is being logged to internal sonde memory and Autosleep RS232 is activated it is only necessary that the value of Twipe Int be less than that of the sample interval selected for the Unattended study Thus if Tw...

Page 317: ...n contaminated or diluted inadvertently The problem could also be due to an internal malfunction of the probe optical system To troubleshoot the problem do not override the cal error return to the Main menu and activate Discrete Sampling in the Run mode Remove the sonde guard and place your thumb or finger over the optics of the probe while observing the data display A high 1000 NTU reading should...

Page 318: ...ve If the probe does not respond contact YSI Technical Support You might see readings during sampling that you think are too jumpy If this occurs the water may be non homogeneous with regard to the size of the suspended particles The jumpiness that you are observing is probably real However if you want to smooth it out you can incrementally increase the Time Constant and Threshold settings in the ...

Page 319: ...Turbidity Measurements Appendix E YSI Incorporated Environmental Monitoring Systems Operations Manual E 6 ...

Page 320: ...l meter is referred to as the secondary measurement device There are three common types of weirs and seven types of flumes Most of these are available in a number of sizes Flow level curves for common types and sizes are already programmed in the sonde so that it is only necessary to describe the primary measuring device to get flow readings If you have a primary measuring device that is not alrea...

Page 321: ...being used The final step is to return to the Report menu and choose the flow and volume units you wish to have in the report Note that the units in the report menu are independent of the units in the Flow Setup menu MANNING EQUATION Once in the Flow Setup menu choose Manning for the Method and then choose which type of channel the measurement is to be done in closed pipe U channel rectangular or ...

Page 322: ... which there is data relating flow to level Once in the Flow Setup menu you must define your Table Do this by entering up to 50 pairs of Head and Flow data points Choose Setup Table Be sure to choose the units that match the data in your table Then enter each pair of points one by one It is not necessary to enter the points in order The software will put them in order There are items on the menu t...

Page 323: ...he pressure sensor properly allows the other sensors to measure the water flowing through the flume and does not significantly alter the geometry of the flume In a flume this means deploying the sonde horizontally If possible deploy the sonde so that the pressure sensor on the side of the sonde is slightly below the lowest level expected Also be aware that the pressure sensor in the sonde is along...

Page 324: ...ld always be attached to the sonde while exposed to the atmosphere to prevent moisture buildup in the vent tube When dry and active the desiccant is a distinct blue color When exhausted it turns to a rose red or pink color The desiccant can be regenerated in an oven See Section 2 10 Care Maintenance and Storage for the proper procedure Avoid bending the cables sharply to prevent the vent tube from...

Page 325: ...e When putting the sonde into service remove the plug to ensure that the sensor in the sonde is vented to the atmosphere INSTALLING THE CANISTER KIT 1 Remove the 1 8 NPT plugs from the stainless steel fittings on the canister 2 Install the 1 8 NPT to 1 8 hose fittings into the stainless steel fittings located on the side of the desiccant canister CAUTION Do not over tighten 3 Place the plugs over ...

Page 326: ...when the sonde is exposed to certain radio frequency fields If you are concerned with these issues consult the Declaration of Conformity page H 2 Specific conditions where temporary sensor problems may occur are listed in this document If you are unable to locate the Declaration of Conformity that was shipped with your instrument contact your local YSI representative or YSI Technical Support in Ye...

Page 327: ...EMC Performance Appendix H YSI Incorporated Environmental Monitoring Systems Operations Manual H 2 ...

Page 328: ... a typical user of 6 series sondes with the YSI 6025 chlorophyll sensors It does not deal with specific instructions for operation of the sondes at the level presented elsewhere in the manual What is the YSI 6025 chlorophyll probe designed to measure The YSI sensor is designed to estimate the phytoplankton content of environmental water Phytoplankton concentrations can be useful in predicting detr...

Page 329: ...s unable to quote an accuracy specification for the in vivo measurement of chlorophyll What calibration methods are available for the YSI 6025 There is only one real method of calibration for the 6025 sensor if accuracy is to be assured versus the true chlorophyll content of the water The sensor must be calibrated with a phytoplankton suspension for which the chlorophyll content is known from extr...

Page 330: ...iurnal cycle even though the same amount of material is present Under this limitation the time of day at which calibration samples are taken would have to be identical to the time of field measurement for the chlorophyll values determined by the two methods to agree consistently This level of synchronization is usually not practical The key point to remember is that in vivo chlorophyll determinati...

Page 331: ...vailable their fluorescence is not likely to emulate in vivo chlorophyll fluorescence any better than the dye standard described above Caution Do not expose the YSI 6025 to calibrant solutions involving any solvent other than water or very dilute aqueous acid mixtures Damage to the sensor under these conditions will not be covered under warranty The Standard Methods procedure generates values desi...

Page 332: ...tivity of the optical system of the YSI 6025 is very stable and is unlikely to show significant drift over time Thus if you are using the dye calibration method with its limitations you may only have to carry out the routine very infrequently Remember that it is basically only useful as a drift check anyway In general you should perform periodic 2 point calibrations using a dye sample more frequen...

Page 333: ...ually recording them or logging them to a computer or YSI 650 MDS display logger You should visually monitor the readings for at least 1 2 minutes to assure stability after the sonde is immersed The second factor is that you should always manually activate the mechanical wiper on the sensor after sonde immersion but before beginning the visual monitoring of the readings The cleaning cycle is neces...

Page 334: ... monitoring data shows a large number of chlorophyll spikes that clearly do not reflect the average phytoplankton content of the water What is causing this effect and is there anything I can do to minimize it This effect shown in the initial chlorophyll readings in the figure below can be caused by the presence of subsurface macroscopic filamentous algae The flexible algal strands can become attac...

Page 335: ...alfunction so that the wiper assembly parks over the probe optics This problem is usually due to the edges of the fluorescent wiper becoming coated with nonfluorescent material such as sediment that in turn prevents the sonde software from detecting the wiper movement If the spikes are due to this factor then the problem will be corrected by manually cleaning the wiper edges so that the fluorescen...

Page 336: ...d factor can then be entered into the sonde software See the example in Section 5 Principles of Operation I suspect that my YSI 6025 chlorophyll sensor is not performing properly What should I do before contacting YSI Technical Support to facilitate resolving the problem You should perform two diagnostic tests to help YSI personnel determine if the YSI 6025 is malfunctioning First determine whethe...

Page 337: ...Chlorophyll Measurements Appendix I YSI Incorporated Environmental Monitoring Systems Operations Manual I 10 ...

Page 338: ...d polarographic oxygen sensor and the YSI ROX optical dissolved oxygen sensor The DOsat Convention In the first convention used for many years in YSI handheld and laboratory instruments and in 6 series sondes the saturation value at the time of calibration in air reflects the value of the barometer that was input in the calibration protocol If the parameter DOsat is active in your 6 series Report ...

Page 339: ...es are obtained at both sites for the DOsat and DOsat Local conventions even though the percent saturation values are significantly different It is important to note that the convention specified for saturation by the British and EU standard REQUIRES that the local barometric pressure be read for every DO data point taken after calibration Under this convention a sensor should ALWAYS read 100 satu...

Page 340: ...bration a method used by some instrument manufacturers the values of DOsat Local will still not be exactly accurate after calibration Activation of the DOsat Local or ODOsat Local Parameter On receipt of your sonde or after upgrading your 6 series software to Version 2 13 or higher the DOsat Local or ODOsat Local parameter will NOT appear in your Report menu To activate the parameter it is necessa...

Page 341: ...Percent Air Saturation Appendix J YSI Incorporated Environmental Monitoring Systems Operations Manual J 4 ...

Page 342: ... other pointing down but it is also possible to use a single PAR sensor if the user prefers that configuration Each Li Cor PAR sensor can be individually configured by Endeco YSI with an internal circuit to have variable sensitivities high usually employed for upwell or downward pointing sensors or low usually employed for downwell or upward pointing sensors After sensitivity configuration the out...

Page 343: ...sors enter the Report menu and make certain that the PAR selections are active as shown below Sensors enabled 1 Time 7 ISE3 NONE 2 Temperature 8 ISE4 NONE 3 Conductivity 9 ISE5 NONE 4 Dissolved Oxy A Turbidity 5 ISE1 pH B Chlorophyll 6 ISE2 Orp C Battery Select option 0 for previous menu 7 Select type 1 ISE3 NH4 2 ISE3 NO3 3 ISE3 Cl 4 ISE3 PAR1 Select option 0 for previous menu 4 Report setup 1 Da...

Page 344: ...ficate were present in the sonde but were configured for both low sensitivity PAR1 and high sensitivity PAR2 the entered gain numbers should be 3 1578 for PAR1 and 0 31578 for PAR2 with both numbers derived from the 315 78 value on the Certificate As noted above once these entries are made the viewed and logged PAR values will be in umoles second m2 of Photon Flux Density For additional informatio...

Page 345: ...PAR Sensor Appendix K YSI Incorporated Environmental Monitoring Systems Operations Manual K 4 ...

Page 346: ...t of the sacrificial anode can also be utilized for 6 series sondes which are deployed in corrosive media particularly seawater or brackish estuarine water In this case the zinc anode is used to prevent corrosion of the stainless steel connector found on the top of most 6 series sondes The instructions below outline the installation procedures for the zinc anode offered by YSI as Model 6182 Kit Th...

Page 347: ...600V2 2 6600EDS V2 2 6600V2 4 6920V2 1 6920V2 2 600XLM and 600 OMS V2 1 Sondes NOTE Before beginning the installation be sure to clean the sonde connector of mud sediment sediments barnacles etc It is important for the surface to be clean in order to make good electrical contact to the zinc anode Figure 4 For the 6600 style sondes first remove the pressure relief battery lid and set it aside for l...

Page 348: ...quired to manually cut a second flat on the other end of the C shaped anode sections Then make certain that fresh batteries have been installed in the sonde Finally with the battery lid in place install the anode sections on the connector being certain that the flats are on the sides where the bail attaches to the battery cover and that the assembly is below the threads of the connector The proper...

Page 349: ...s for 600XLM 600 OMS V2 1 and 6920 style sondes A new plastic cable tie will be needed to re secure the zinc WARNING The zinc anode must not interfere with the ability to properly attach of the pressure cap to the sonde connector the cap MUST be threaded all the way down to prevent leaks into the connector Therefore the anode must also NOT be placed over the threads of the connector ...

Page 350: ...DO sensors including the YSI 6150 this Stern Volmer relationship Tzero T 1 versus O2 pressure is not strictly linear particularly at higher oxygen pressures and the data must be processed using analysis by polynomial non linear regression rather than the simple linear regression used for most polarographic oxygen sensors Fortunately the non linearity does not change significantly with time so that...

Page 351: ...ensor contact YSI Technical Support They will be able to evaluate the compatibility of your sonde with the ROX sensor by giving you a few simple software commands to issue at the sign of your sonde If I want to use the new ROX DO Sensor with my existing sonde what else do I have to do In order to use the ROX technology on an existing YSI 6 series sonde you must do the following Upgrade the firmwar...

Page 352: ...alled on the 6150 probe and NOT a function of the probe i e the constants reflect the characteristics of the sensor membrane and NOT the probe When a 6150 probe is purchased from YSI it already has a sensor membrane installed and the constants of that membrane are transferred automatically to the sonde PCB when the sensor is run for the first time After transfer the constants can be viewed by acce...

Page 353: ...ration how do I generate a zero oxygen medium Two methods are generally used to provide a zero oxygen environment 1 Place the ROX sensor in a vessel which is filled with a flowing inert gas such as nitrogen gas 2 Place the ROX sensor in an aqueous solution of sodium sulfite at a concentration of approximately 2 g L The following qualifiers apply to the zero point calibration methods If you use nit...

Page 354: ... sensors are present in the sonde and will be about 15 seconds per optical sensor Thus lag will be about 15 seconds for a sonde with two optical sensors and about 1 minute for a sonde with four optical sensors How does the ROX sensor deal with fouling in field studies The ROX sensor has a wiper similar to those used on all YSI optical sensors turbidity chlorophyll rhodamine WT and blue green algae...

Page 355: ...shion to K1 pressing Enter to confirm each entry If no error message is encountered after confirming the C value then you have made all entries correctly and the proper constants will be transferred automatically into the sensor system for correct calculation of dissolved oxygen If an error message is displayed after entry of C then you have made an error or errors in entering the code Following t...

Page 356: ...rehydration is complete store the probe in either water or water saturated air prior to calibration and deployment Once the rehydration has been performed the sensor should be returned to its original performance specification Can I use alcohol or other organic solvents to clean my sensor membrane Absolutely NOT Alcohol will dissolve the outer paint layer of the membrane assembly and other organic...

Page 357: ...the sensor specification of 1 of the reading This error will be further reduced if other optical probes are present since the time for them to be wiped plus their time constants will further increase the time between the wiping of the ROX sensor and the time a point is logged to internal memory Note that the ROX sensor is ALWAYS WIPED FIRST no matter what optical port it is installed in as long as...

Page 358: ...n Discrete Sample or Unattended Sample studies becomes jumpier than normal In addition if your membrane surface has suffered major damage as evidenced by loss of more than 10 of the outer paint layer then even if the readings are stable and you do not see calibration errors it is probably time to change your membrane assembly What should I do if I don t think that my ROX sensor is functioning prop...

Page 359: ...ROX Optical DO Sensor Appendix M YSI Incorporated Environmental Monitoring Systems Operations Manual M 10 ...

Page 360: ...nabled start up to NMEA the sonde will revert back to outputting NMEA strings after you reset or power cycle the sonde The format of the NEMA string is YSI Code 1 Value 1 Code 2 Value 2 XX Code is the YSI defined parameter code See table below Value is the value of the parameter XX is a check computed by a bitwise exclusive or xor of all characters between and non inclusive The XX value is sent as...

Page 361: ...ss psi 22 Depth meters 23 Depth feet 118 Flow ft3 sec 166 Flow ft3 min 167 Flow ft3 hour 168 Flow ft3 day 164 Flow gal sec 119 Flow gal min 165 Flow gal hour 120 Flow Mgal day 121 Flow m3 sec 169 Flow m3 min 170 Flow m3 hour 171 Flow m3 day 122 Flow L s 172 Flow AF day 123 Volume ft3 124 Volume gal 173 Volume Mgal 125 Volume m3 126 Volume L 174 Volume acre ft 18 pH 17 pH mV 19 Orp mV 48 NH4 N mg L...

Page 362: ...vironmental Monitoring Systems Operations Manual N 3 204 Rhodamine ug L 211 ODOsat 214 ODOsat Local 212 ODO mg L 215 BGA PC cells mL 216 BGA PC RFU 217 BGA PE cells mL 218 BGA PE RFU 98 Gnd Hz 99 Scale Hz 100 Prescmp 32 Density kg m3 28 Battery volts ...

Page 363: ...NMEA Applications Appendix N YSI Incorporated Environmental Monitoring Systems Operations Manual N 4 ...

Page 364: ...Computer Interface RS 232C SDI 12 Internal logging memory size 384 kilobytes 150 000 individual parameter readings Power 8 C size Alkaline Batteries or External 12 VDC Battery Life Approximately 75 days at 20 C at a 15 minute logging interval and temperature conductivity pH ORP Rapid Pulse DO with a 60 second DO warm up time and two optical probes other than ROX DO active Approximately 70 days at ...

Page 365: ...tely 70 days at 20 C at a 15 minute logging interval with temperature conductivity pH ORP ROX Optical DO and one other optical probe active Battery life is heavily dependent on sensor configuration and is given above for a typical sensor ensemble If you have a different sensor configuration set up your sonde for a deployment in the Run Unattended menu and check the projected approximate battery li...

Page 366: ... BGA PC or BGA PE Note that Rapid Pulse and ROX DO sensors cannot be activated simultaneously Operating Environment Medium fresh sea or polluted water Temperature 5 to 50 C for most sensors Depth 0 to 200 feet 61 meters Storage Temperature 40 to 60 C for sonde and all sensors except pH pH ORP ISE and optical sensors 10 to 60 C for pH pH ORP ISE and optical sensors Material Polyurethane PVC Stainle...

Page 367: ... memory size 384 kilobytes 150 000 individual parameter readings Power 8 AA size Alkaline Batteries or External 12 VDC Battery Life Approximately 32 days at 20 C at a 15 minute logging interval with ROX Optical DO another optical sensor turbidity chlorophyll Rhodamine WT BGA BC or BGA PE temperature conductivity and pH active Battery life is heavily dependent on sensor configuration and is given a...

Page 368: ...P one ion selective electrode ammonium nitrate or chloride and Depth shallow medium shallow vented Two total optical sensors ROX Optical DO Turbidity Chlorophyll Rhodamine WT BGA PC or BGA PE Operating Environment Medium fresh sea or polluted water Temperature 5 to 50 C for most sensors Depth 0 to 200 feet 61 meters Storage Temperature 40 to 60 C for sonde and all sensors except pH pH ORP ISE and ...

Page 369: ...y 30 days at 20 C at a 15 minute logging interval with temperature conductivity pH ORP and Rapid Pulse DO with a 60 second DO warm up time active Battery life is heavily dependent on sensor configuration and is given above for a typical sensor ensemble If you have a different sensor configuration set up your sonde for a deployment in the Run Unattended menu and check the projected approximate batt...

Page 370: ...l PVC Stainless Steel Diameter 1 65 inches 4 2 cm Length 14 75 inches 37 5 cm from bottom of probe guard to top of connector with no depth and no weight Add 2 inches 5 1 cm for depth add 0 75 inches 1 9 cm for bottom weight Weight 1 1 pounds 0 50 kg without weight Computer Interface RS 232C SDI 12 Internal logging memory size 384 kilobytes 150 000 individual parameter readings Power External 12 VD...

Page 371: ...ure 40 to 60 C without optical sensor installed 10 to 60 C with optical sensor installed Material PVC Stainless Steel Diameter 1 65 inches 4 2 cm Length 21 25 inches 54 0 cm from top of connector to bottom of probe guard without depth bottom weight and battery option Add 3 75 inches 9 5 cm for battery option Add 2 0 inches 5 1 cm for depth Add 0 75 inches 1 9 cm for weight Weight 1 78 pounds 0 81 ...

Page 372: ...000 individual parameter readings Power 4 AA size Alkaline Batteries or External 12 VDC Battery Life Approximately 180 days at 20 C with a 30 minute sample interval Battery life is heavily dependent on sensor configuration and is given above for a typical sensor ensemble If you have a different sensor configuration set up your sonde for a deployment in the Run Unattended menu and check the project...

Page 373: ... 50 C Depth 200 meters Rapid Pulse Dissolved Oxygen mg L Calculated from air saturation temperature and salinity Sensor Type Rapid Pulse Clark type polarographic Range 0 to 50 mg L Accuracy 0 to 20 mg L 2 of the reading or 0 2 mg L whichever is greater 20 to 50 mg L 6 of the reading Resolution 0 01 mg L Temperature Range 5 to 50 C Depth 200 meters ROX Optical Dissolved Oxygen saturation Sensor Typ...

Page 374: ... chlorophyll a concentration determined through extractions Resolution 0 1 µg L Chl 0 1 RFU Temperature Range 5 to 50 C Depth 61 meters Phycocyanin Blue green Algae BGA PC Sensor type Optical fluorescence with mechanical cleaning Range Approximately 0 to 280 000 cells mL of BGA See Principles of Operation section for explanation of estimate 0 100 Relative Fluorescence Units RFU Accuracy No specifi...

Page 375: ... 0 of reading or 0 1 ppt whichever is greater Resolution 0 01 ppt Temperature Range 5 to 50 C Depth 200 meters Nitrate Nitrogen Sensor Type Ion selective electrode Range 0 200 mg L N Accuracy 10 of reading or 2 mg L whichever is greater Resolution 0 001 mg L N to 1 mg L N range dependent Temperature Range 5 to 50 C Depth 15 meters Ammonium Nitrogen Sensor Type Ion selective electrode Range 0 200 m...

Page 376: ...pth 15 meters Chloride Sensor Type Solid state ion selective electrode Range 0 1000 mg L Accuracy 15 of reading or 5 mg L whichever is greater Resolution 0 001 mg L to 1 mg L range dependent Temperature Range 5 to 50 C Depth 15 meters SOFTWARE SPECIFICATIONS EcoWatch for Windows included IBM PC compatible computer with CD ROM drive and with a 386 processor or better running Windows software Compat...

Page 377: ...Item 069300 Drawing A69300 Revision H November 2011 1700 1725 Brannum Lane Yellow Springs Ohio 45387 USA 800 765 4974 937 767 7241 FAX 937 767 9320 Website www ysi com E mail environmental ysi com ...

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