15
815#
6HWXS
5.2.1
Monitor
As described in Section 2, Installation, there are six menu selections under Monitor Setup that you must
consider. Briefly, they are:
External Pump Purge Time........Time in seconds required to deliver fresh sample from your source to the
External Sampling Chamber
Station # .....................................Station number where the Sipper Tube will go for a monitor sample,
usually station #5 the external sampling chamber
Interval.......................................Time in minutes between monitor samples
PreCal ........................................Time in minutes before the monitor sample when an autocalibration will
be initiated
Flow ...........................................The flow rate of the monitor pump in uL/min
Antiseptic ...................................Antiseptic cycle on or off
Flow Rate -vs- Purge Time
The flow rate and purge time settings must be such that a sufficient volume of sample is pumped during
each cycle to completely purge the external chamber and system tubing. This is especially important when
an antiseptic is used since the antiseptic may damage the enzyme membrane if aspirated by the sipper
needle. Figure 10 shows the flow rates and minimum time needed to purge the tubing lines from the
solenoid valve to the external sample chamber. These values are based upon a 6-fold volume turnover.
Additional time may be necessary to purge the line from the sample source to the solenoid valve.
Figure 10 Minimum Purge Time
5.2.2
Antiseptic
The antiseptic solution may be defined by the user. Typical solutions might include 1% sodium hydroxide
or 0.25% hypochlorite in reagent water.
Flow Rate
uL/min
Purge Time
Seconds
Flow Rate
uL/min
Purge Time
Seconds
100
900
1400
65
200
450
1500
60
300
300
1600
57
400
225
1700
53
500
180
1800
50
600
150
1900
48
700
129
2000
45
800
113
2100
43
900
101
2200
41
1000
90
2300
39
1100
82
2400
38
1200
75
2500
36
1300
69
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